ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations

Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muraseva Dinara S., Guseva Alexandra A.
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/3747b7ce4b914347848bebda19febbb2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3747b7ce4b914347848bebda19febbb2
record_format	dspace
spelling	oai:doaj.org-article:3747b7ce4b914347848bebda19febbb22021-11-08T15:17:37ZISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations2117-445810.1051/bioconf/20213800082https://doaj.org/article/3747b7ce4b914347848bebda19febbb22021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/10/bioconf_napd2021_00082.pdfhttps://doaj.org/toc/2117-4458Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was assessed on a point scale. When assessing the primers, the number of reproducible amplified DNA fragments, the clarity and brightness of the obtained fragments, and only distinctive bands were taken into account. As a result, 10 ISSR markers were selected that are the most informative for assessing the population diversity of the species.Muraseva Dinara S.Guseva Alexandra A.EDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 38, p 00082 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Muraseva Dinara S. Guseva Alexandra A. ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
description	Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was assessed on a point scale. When assessing the primers, the number of reproducible amplified DNA fragments, the clarity and brightness of the obtained fragments, and only distinctive bands were taken into account. As a result, 10 ISSR markers were selected that are the most informative for assessing the population diversity of the species.
format	article
author	Muraseva Dinara S. Guseva Alexandra A.
author_facet	Muraseva Dinara S. Guseva Alexandra A.
author_sort	Muraseva Dinara S.
title	ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
title_short	ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
title_full	ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
title_fullStr	ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
title_full_unstemmed	ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
title_sort	issr primer screening for analysis of genetic diversity among scutellaria tuvensis (lamiaceae) populations
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/3747b7ce4b914347848bebda19febbb2
work_keys_str_mv	AT murasevadinaras issrprimerscreeningforanalysisofgeneticdiversityamongscutellariatuvensislamiaceaepopulations AT gusevaalexandraa issrprimerscreeningforanalysisofgeneticdiversityamongscutellariatuvensislamiaceaepopulations
_version_	1718442030525317120

ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations

Ejemplares similares