Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation

Delivery of hydrophilic molecules through the skin using electroporation is a promising alternative approach to intradermal injection. Recently, we developed a two-in-one electrode/reservoir material composed of carbon nanotubes and agarose hydrogel. In this work, we evaluated the potential of the d...

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Autores principales:	Juliette Simon, Bastien Jouanmiqueou, Marie-Pierre Rols, Emmanuel Flahaut, Muriel Golzio
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	skin electroporation macromolecule delivery carbon nanotubes hydrogel composite Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/374b1b36445d4f95a84e71716273723a
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id	oai:doaj.org-article:374b1b36445d4f95a84e71716273723a
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spelling	oai:doaj.org-article:374b1b36445d4f95a84e71716273723a2021-11-25T18:40:49ZTransdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation10.3390/pharmaceutics131118051999-4923https://doaj.org/article/374b1b36445d4f95a84e71716273723a2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1805https://doaj.org/toc/1999-4923Delivery of hydrophilic molecules through the skin using electroporation is a promising alternative approach to intradermal injection. Recently, we developed a two-in-one electrode/reservoir material composed of carbon nanotubes and agarose hydrogel. In this work, we evaluated the potential of the device to achieve non-invasive transdermal drug delivery using skin electroporation. As it involved an electrode configuration different from the literature, critical questions were raised. First, we demonstrated the efficiency of the device to permeabilize the skin of hairless mice, as observed by propidium iodide (PI) uptake in the nuclei of the epidermis cells through macro fluorescence imaging and histology. Application of Lucifer yellow (LY) at different times after unipolar electroporation treatment demonstrated the partial reversibility of the skin permeabilization after 30 min, and as such, that barrier function properties tended to be restored. We uncovered, for the first time to our knowledge, an intrinsic asymmetry of permeation pathways generated in the <i>stratum corneum</i> during treatment. Electrophoresis was here the main driving force for macromolecule delivery, but it competed with passive diffusion through the generated aqueous pathways for smaller molecules. Finally, we validated 4 kDa dextran labelled with fluorescein isothiocyanate (FD4) as a model molecule to optimize the electrical parameters, needed to improve macromolecule delivery.Juliette SimonBastien JouanmiqueouMarie-Pierre RolsEmmanuel FlahautMuriel GolzioMDPI AGarticleskin electroporationmacromolecule deliverycarbon nanotubeshydrogel compositePharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1805, p 1805 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	skin electroporation macromolecule delivery carbon nanotubes hydrogel composite Pharmacy and materia medica RS1-441
spellingShingle	skin electroporation macromolecule delivery carbon nanotubes hydrogel composite Pharmacy and materia medica RS1-441 Juliette Simon Bastien Jouanmiqueou Marie-Pierre Rols Emmanuel Flahaut Muriel Golzio Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation
description	Delivery of hydrophilic molecules through the skin using electroporation is a promising alternative approach to intradermal injection. Recently, we developed a two-in-one electrode/reservoir material composed of carbon nanotubes and agarose hydrogel. In this work, we evaluated the potential of the device to achieve non-invasive transdermal drug delivery using skin electroporation. As it involved an electrode configuration different from the literature, critical questions were raised. First, we demonstrated the efficiency of the device to permeabilize the skin of hairless mice, as observed by propidium iodide (PI) uptake in the nuclei of the epidermis cells through macro fluorescence imaging and histology. Application of Lucifer yellow (LY) at different times after unipolar electroporation treatment demonstrated the partial reversibility of the skin permeabilization after 30 min, and as such, that barrier function properties tended to be restored. We uncovered, for the first time to our knowledge, an intrinsic asymmetry of permeation pathways generated in the <i>stratum corneum</i> during treatment. Electrophoresis was here the main driving force for macromolecule delivery, but it competed with passive diffusion through the generated aqueous pathways for smaller molecules. Finally, we validated 4 kDa dextran labelled with fluorescein isothiocyanate (FD4) as a model molecule to optimize the electrical parameters, needed to improve macromolecule delivery.
format	article
author	Juliette Simon Bastien Jouanmiqueou Marie-Pierre Rols Emmanuel Flahaut Muriel Golzio
author_facet	Juliette Simon Bastien Jouanmiqueou Marie-Pierre Rols Emmanuel Flahaut Muriel Golzio
author_sort	Juliette Simon
title	Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation
title_short	Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation
title_full	Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation
title_fullStr	Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation
title_full_unstemmed	Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation
title_sort	transdermal delivery of macromolecules using two-in-one nanocomposite device for skin electroporation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/374b1b36445d4f95a84e71716273723a
work_keys_str_mv	AT juliettesimon transdermaldeliveryofmacromoleculesusingtwoinonenanocompositedeviceforskinelectroporation AT bastienjouanmiqueou transdermaldeliveryofmacromoleculesusingtwoinonenanocompositedeviceforskinelectroporation AT mariepierrerols transdermaldeliveryofmacromoleculesusingtwoinonenanocompositedeviceforskinelectroporation AT emmanuelflahaut transdermaldeliveryofmacromoleculesusingtwoinonenanocompositedeviceforskinelectroporation AT murielgolzio transdermaldeliveryofmacromoleculesusingtwoinonenanocompositedeviceforskinelectroporation
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Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation

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