Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings

Abstract Despite recent interest in using zebrafish in human disease studies, sparked by their economics, fecundity, easy handling, and homologies to humans, the electrophysiological tools or methods for zebrafish are still inaccessible. Although zebrafish exhibit more significant larval–adult duali...

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Autores principales:	Sung-Joon Cho, Donghak Byun, Tai-Seung Nam, Seok-Yong Choi, Byung-Geun Lee, Myeong-Kyu Kim, Sohee Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/374d8c260cce43598ffd8ddcbaa9d42e
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spelling	oai:doaj.org-article:374d8c260cce43598ffd8ddcbaa9d42e2021-12-02T12:32:44ZZebrafish as an animal model in epilepsy studies with multichannel EEG recordings10.1038/s41598-017-03482-62045-2322https://doaj.org/article/374d8c260cce43598ffd8ddcbaa9d42e2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03482-6https://doaj.org/toc/2045-2322Abstract Despite recent interest in using zebrafish in human disease studies, sparked by their economics, fecundity, easy handling, and homologies to humans, the electrophysiological tools or methods for zebrafish are still inaccessible. Although zebrafish exhibit more significant larval–adult duality than any other animal, most electrophysiological studies using zebrafish are biased by using larvae these days. The results of larval studies not only differ from those conducted with adults but also are unable to delicately manage electroencephalographic montages due to their small size. Hence, we enabled non-invasive long-term multichannel electroencephalographic recording on adult zebrafish using custom-designed electrodes and perfusion system. First, we exploited demonstration of long-term recording on pentylenetetrazole-induced seizure models, and the results were quantified. Second, we studied skin–electrode impedance, which is crucial to the quality of signals. Then, seizure propagations and gender differences in adult zebrafish were exhibited for the first time. Our results provide a new pathway for future neuroscience research using zebrafish by overcoming the challenges for aquatic organisms such as precision, serviceability, and continuous water seepage.Sung-Joon ChoDonghak ByunTai-Seung NamSeok-Yong ChoiByung-Geun LeeMyeong-Kyu KimSohee KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sung-Joon Cho Donghak Byun Tai-Seung Nam Seok-Yong Choi Byung-Geun Lee Myeong-Kyu Kim Sohee Kim Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings
description	Abstract Despite recent interest in using zebrafish in human disease studies, sparked by their economics, fecundity, easy handling, and homologies to humans, the electrophysiological tools or methods for zebrafish are still inaccessible. Although zebrafish exhibit more significant larval–adult duality than any other animal, most electrophysiological studies using zebrafish are biased by using larvae these days. The results of larval studies not only differ from those conducted with adults but also are unable to delicately manage electroencephalographic montages due to their small size. Hence, we enabled non-invasive long-term multichannel electroencephalographic recording on adult zebrafish using custom-designed electrodes and perfusion system. First, we exploited demonstration of long-term recording on pentylenetetrazole-induced seizure models, and the results were quantified. Second, we studied skin–electrode impedance, which is crucial to the quality of signals. Then, seizure propagations and gender differences in adult zebrafish were exhibited for the first time. Our results provide a new pathway for future neuroscience research using zebrafish by overcoming the challenges for aquatic organisms such as precision, serviceability, and continuous water seepage.
format	article
author	Sung-Joon Cho Donghak Byun Tai-Seung Nam Seok-Yong Choi Byung-Geun Lee Myeong-Kyu Kim Sohee Kim
author_facet	Sung-Joon Cho Donghak Byun Tai-Seung Nam Seok-Yong Choi Byung-Geun Lee Myeong-Kyu Kim Sohee Kim
author_sort	Sung-Joon Cho
title	Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings
title_short	Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings
title_full	Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings
title_fullStr	Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings
title_full_unstemmed	Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings
title_sort	zebrafish as an animal model in epilepsy studies with multichannel eeg recordings
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/374d8c260cce43598ffd8ddcbaa9d42e
work_keys_str_mv	AT sungjooncho zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings AT donghakbyun zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings AT taiseungnam zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings AT seokyongchoi zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings AT byunggeunlee zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings AT myeongkyukim zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings AT soheekim zebrafishasananimalmodelinepilepsystudieswithmultichanneleegrecordings
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Zebrafish as an animal model in epilepsy studies with multichannel EEG recordings

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