Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles

Giulietta Sinigaglia1, Massimiliano Magro1, Giovanni Miotto1, Sara Cardillo1, Enzo Agostinelli2,3, Radek Zboril4, Eris Bidollari2,3, Fabio Vianello11Department of Biological Chemistry, University of Padua, Padua, Italy; 2Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Science...

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Autores principales:	Bidollari E, Zboril R, Agostinelli E, Cardillo S, Vianello F, Miotto G, Magro M, Sinigaglia G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/37628b3f9cb04a44b60665b8c1d7ba78
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spelling	oai:doaj.org-article:37628b3f9cb04a44b60665b8c1d7ba782021-12-02T02:28:06ZCatalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles1176-91141178-2013https://doaj.org/article/37628b3f9cb04a44b60665b8c1d7ba782012-05-01T00:00:00Zhttp://www.dovepress.com/catalytically-active-bovine-serum-amine-oxidase-bound-to-fluorescent-a-a9806https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Giulietta Sinigaglia1, Massimiliano Magro1, Giovanni Miotto1, Sara Cardillo1, Enzo Agostinelli2,3, Radek Zboril4, Eris Bidollari2,3, Fabio Vianello11Department of Biological Chemistry, University of Padua, Padua, Italy; 2Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences &quot;A. Rossi Fanelli&quot;, SAPIENZA University of Rome, Rome, Italy; 3CNR, Institute Biology and Molecular Pathology, Rome, Italy; 4Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Palacky University, Olomouc, Czech RepublicAbstract: Novel superparamagnetic surface-active maghemite nanoparticles (SAMNs) characterized by a diameter of 10 &plusmn; 2 nm were modified with bovine serum amine oxidase, which used rhodamine B isothiocyanate (RITC) adduct as a fluorescent spacer-arm. A fluorescent and magnetically drivable adduct comprised of bovine serum copper-containing amine oxidase (SAMN&ndash;RITC&ndash;BSAO) that immobilized on the surface of specifically functionalized magnetic nanoparticles was developed. The multifunctional nanomaterial was characterized using transmission electron microscopy, infrared spectroscopy, mass spectrometry, and activity measurements. The results of this study demonstrated that bare magnetic nanoparticles form stable colloidal suspensions in aqueous solutions. The maximum binding capacity of bovine serum amine oxidase was approximately 6.4 mg g-1 nanoparticles. The immobilization procedure reduced the catalytic activity of the native enzyme to 30% &plusmn; 10% and the Michaelis constant was increased by a factor of 2. We suggest that the SAMN&ndash;RITC&ndash;BSAO complex, characterized by a specific activity of 0.81 IU g-1, could be used in the presence of polyamines to create a fluorescent magnetically drivable H2O2 and aldehydes-producing system. Selective tumor cell destruction is suggested as a potential future application of this system.Keywords: amine oxidase, hydrogen peroxide production, superparamagnetic nanoparticles, rhodamine isothiocyanate, fluorescent nanoparticles, fluorescent nanocatalystBidollari EZboril RAgostinelli ECardillo SVianello FMiotto GMagro MSinigaglia GDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2249-2259 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Bidollari E Zboril R Agostinelli E Cardillo S Vianello F Miotto G Magro M Sinigaglia G Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
description	Giulietta Sinigaglia1, Massimiliano Magro1, Giovanni Miotto1, Sara Cardillo1, Enzo Agostinelli2,3, Radek Zboril4, Eris Bidollari2,3, Fabio Vianello11Department of Biological Chemistry, University of Padua, Padua, Italy; 2Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences &quot;A. Rossi Fanelli&quot;, SAPIENZA University of Rome, Rome, Italy; 3CNR, Institute Biology and Molecular Pathology, Rome, Italy; 4Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Palacky University, Olomouc, Czech RepublicAbstract: Novel superparamagnetic surface-active maghemite nanoparticles (SAMNs) characterized by a diameter of 10 &plusmn; 2 nm were modified with bovine serum amine oxidase, which used rhodamine B isothiocyanate (RITC) adduct as a fluorescent spacer-arm. A fluorescent and magnetically drivable adduct comprised of bovine serum copper-containing amine oxidase (SAMN&ndash;RITC&ndash;BSAO) that immobilized on the surface of specifically functionalized magnetic nanoparticles was developed. The multifunctional nanomaterial was characterized using transmission electron microscopy, infrared spectroscopy, mass spectrometry, and activity measurements. The results of this study demonstrated that bare magnetic nanoparticles form stable colloidal suspensions in aqueous solutions. The maximum binding capacity of bovine serum amine oxidase was approximately 6.4 mg g-1 nanoparticles. The immobilization procedure reduced the catalytic activity of the native enzyme to 30% &plusmn; 10% and the Michaelis constant was increased by a factor of 2. We suggest that the SAMN&ndash;RITC&ndash;BSAO complex, characterized by a specific activity of 0.81 IU g-1, could be used in the presence of polyamines to create a fluorescent magnetically drivable H2O2 and aldehydes-producing system. Selective tumor cell destruction is suggested as a potential future application of this system.Keywords: amine oxidase, hydrogen peroxide production, superparamagnetic nanoparticles, rhodamine isothiocyanate, fluorescent nanoparticles, fluorescent nanocatalyst
format	article
author	Bidollari E Zboril R Agostinelli E Cardillo S Vianello F Miotto G Magro M Sinigaglia G
author_facet	Bidollari E Zboril R Agostinelli E Cardillo S Vianello F Miotto G Magro M Sinigaglia G
author_sort	Bidollari E
title	Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
title_short	Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
title_full	Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
title_fullStr	Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
title_full_unstemmed	Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
title_sort	catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/37628b3f9cb04a44b60665b8c1d7ba78
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Catalytically active bovine serum amine oxidase bound to fluorescent and magnetically drivable nanoparticles

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