Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China

Wen-Qi Xu,1,2 Jing-Wei Liu,1,2 Xiao-Yu Zhu,1,2 Xiao-Li Zheng,1,2 Kai Chen,1,2 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,2 1STD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, People’s Republic of...

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Autores principales:	Xu WQ, Liu JW, Zhu XY, Zheng XL, Chen K, Chen XS, Yin YP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	neisseria gonorrhoeae disk diffusion test gradient strip test antimicrobial susceptibility. Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/37642181696546eca80e2929b2170449
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spelling	oai:doaj.org-article:37642181696546eca80e2929b21704492021-12-02T19:17:36ZEvaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China1178-6973https://doaj.org/article/37642181696546eca80e2929b21704492021-12-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-the-accuracy-of-various-disks-and-strips-for-rapid-cultu-peer-reviewed-fulltext-article-IDRhttps://doaj.org/toc/1178-6973Wen-Qi Xu,1,2 Jing-Wei Liu,1,2 Xiao-Yu Zhu,1,2 Xiao-Li Zheng,1,2 Kai Chen,1,2 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,2 1STD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of ChinaCorrespondence: Yue-Ping YinSTD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 12, Jiangwangmiao Street, Nanjing, 210042, People’s Republic of ChinaTel/Fax +86 258 547 8024Email yinyp@ncstdlc.orgPurpose: Neisseria gonorrhoeae, resistant to the first-line treatment option ceftriaxone, is widespread in China from 2016. Nowadays, diverse reagents of disks and strips for rapid gonococcal antimicrobial susceptibility tests used in clinics are culture-based disks diffusion and gradient strips methods. This study aimed to evaluate the accuracy, quality, and availability of almost all disks and strips acquired in the Chinese market and serve as a reference for clinical selection.Methods: We tested the performance of 15 commercial disks and 9 commercial gradient strips acquired in China, compared with traditional agar dilution method. The overall performance was evaluated by the categorical agreement. The reagent accuracy of gradient strips was assessed by the essential agreement.Results: A total of 167 gonococcal isolates were used to evaluate antimicrobial disks from three brands. The overall categorical agreements were 71.7% to 81.8% for ceftriaxone, less than 58% for cefixime, 100% for spectinomycin, over 98% for ciprofloxacin, below 70.5% for penicillin, and 73.3% to 81.8% for tetracycline. A total of 81 isolates were tested for different gradient strips. Categorical agreements were over 96% for ceftriaxone, 86.2% for azithromycin, 62.3% to 67.1% for penicillin, 41.9% to 67.5% for tetracycline, and 95% for ciprofloxacin. Essential agreements were 57.7% to 87.3% for ceftriaxone, 70% for azithromycin, 64.9% to 68.4% for penicillin, 51.8% to 71.2% for tetracycline, and 91.3% for ciprofloxacin.Conclusion: Rapid test reagents of disks and strips based on gonococcal culture have suboptimal performance. Disk diffusion for spectinomycin or ciprofloxacin can be recommended for clinical individualized prescription. The gradient strips are of great value to identify ceftriaxone-resistant gonococcal strains. Furthermore, abundant improvements are required for many reagents to further optimize their accuracy till the fulfillment of molecular detection.Keywords: Neisseria gonorrhoeae, disk diffusion test, gradient strip test, antimicrobial susceptibilityXu WQLiu JWZhu XYZheng XLChen KChen XSYin YPDove Medical Pressarticleneisseria gonorrhoeaedisk diffusion testgradient strip testantimicrobial susceptibility.Infectious and parasitic diseasesRC109-216ENInfection and Drug Resistance, Vol Volume 14, Pp 5131-5136 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neisseria gonorrhoeae disk diffusion test gradient strip test antimicrobial susceptibility. Infectious and parasitic diseases RC109-216
spellingShingle	neisseria gonorrhoeae disk diffusion test gradient strip test antimicrobial susceptibility. Infectious and parasitic diseases RC109-216 Xu WQ Liu JW Zhu XY Zheng XL Chen K Chen XS Yin YP Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China
description	Wen-Qi Xu,1,2 Jing-Wei Liu,1,2 Xiao-Yu Zhu,1,2 Xiao-Li Zheng,1,2 Kai Chen,1,2 Xiang-Sheng Chen,1,2 Yue-Ping Yin1,2 1STD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, People’s Republic of China; 2STD Reference Laboratory, National Center for Sexually Transmitted Diseases Control, Chinese Center for Disease Control and Prevention, Nanjing, People’s Republic of ChinaCorrespondence: Yue-Ping YinSTD Reference Laboratory, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 12, Jiangwangmiao Street, Nanjing, 210042, People’s Republic of ChinaTel/Fax +86 258 547 8024Email yinyp@ncstdlc.orgPurpose: Neisseria gonorrhoeae, resistant to the first-line treatment option ceftriaxone, is widespread in China from 2016. Nowadays, diverse reagents of disks and strips for rapid gonococcal antimicrobial susceptibility tests used in clinics are culture-based disks diffusion and gradient strips methods. This study aimed to evaluate the accuracy, quality, and availability of almost all disks and strips acquired in the Chinese market and serve as a reference for clinical selection.Methods: We tested the performance of 15 commercial disks and 9 commercial gradient strips acquired in China, compared with traditional agar dilution method. The overall performance was evaluated by the categorical agreement. The reagent accuracy of gradient strips was assessed by the essential agreement.Results: A total of 167 gonococcal isolates were used to evaluate antimicrobial disks from three brands. The overall categorical agreements were 71.7% to 81.8% for ceftriaxone, less than 58% for cefixime, 100% for spectinomycin, over 98% for ciprofloxacin, below 70.5% for penicillin, and 73.3% to 81.8% for tetracycline. A total of 81 isolates were tested for different gradient strips. Categorical agreements were over 96% for ceftriaxone, 86.2% for azithromycin, 62.3% to 67.1% for penicillin, 41.9% to 67.5% for tetracycline, and 95% for ciprofloxacin. Essential agreements were 57.7% to 87.3% for ceftriaxone, 70% for azithromycin, 64.9% to 68.4% for penicillin, 51.8% to 71.2% for tetracycline, and 91.3% for ciprofloxacin.Conclusion: Rapid test reagents of disks and strips based on gonococcal culture have suboptimal performance. Disk diffusion for spectinomycin or ciprofloxacin can be recommended for clinical individualized prescription. The gradient strips are of great value to identify ceftriaxone-resistant gonococcal strains. Furthermore, abundant improvements are required for many reagents to further optimize their accuracy till the fulfillment of molecular detection.Keywords: Neisseria gonorrhoeae, disk diffusion test, gradient strip test, antimicrobial susceptibility
format	article
author	Xu WQ Liu JW Zhu XY Zheng XL Chen K Chen XS Yin YP
author_facet	Xu WQ Liu JW Zhu XY Zheng XL Chen K Chen XS Yin YP
author_sort	Xu WQ
title	Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China
title_short	Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China
title_full	Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China
title_fullStr	Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China
title_full_unstemmed	Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China
title_sort	evaluation of the accuracy of various disks and strips for rapid culture-based gonococcal antimicrobial susceptibility screening tests in china
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/37642181696546eca80e2929b2170449
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Evaluation of the Accuracy of Various Disks and Strips for Rapid Culture-Based Gonococcal Antimicrobial Susceptibility Screening Tests in China

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