RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.

Verticillium longisporum is a soil-borne pathogen with a preference for plants within the family Brassicaceae. Following invasion of the roots, the fungus proliferates in the plant vascular system leading to stunted plant growth, chlorosis and premature senescence. RabGTPases have been demonstrated...

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Autores principales:	Jonas Roos, Sarosh Bejai, Shinichi Oide, Christina Dixelius
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/376c2d69b5d24fc0bac768a68ae42379
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spelling	oai:doaj.org-article:376c2d69b5d24fc0bac768a68ae423792021-11-18T08:33:50ZRabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.1932-620310.1371/journal.pone.0088187https://doaj.org/article/376c2d69b5d24fc0bac768a68ae423792014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24505423/?tool=EBIhttps://doaj.org/toc/1932-6203Verticillium longisporum is a soil-borne pathogen with a preference for plants within the family Brassicaceae. Following invasion of the roots, the fungus proliferates in the plant vascular system leading to stunted plant growth, chlorosis and premature senescence. RabGTPases have been demonstrated to play a crucial role in regulating multiple responses in plants. Here, we report on the identification and characterization of the Rab GTPase-activating protein RabGAP22 gene from Arabidopsis, as an activator of multiple components in the immune responses to V. longisporum. RabGAP22Pro :GUS transgenic lines showed GUS expression predominantly in root meristems, vascular tissues and stomata, whereas the RabGAP22 protein localized in the nucleus. Reduced RabGAP22 transcript levels in mutants of the brassinolide (BL) signaling gene BRI1-associated receptor kinase 1, together with a reduction of fungal proliferation following BL pretreatment, suggested RabGAP22 to be involved in BL-mediated responses. Pull-down assays revealed serine:glyoxylate aminotransferase (AGT1) as an interacting partner during V. longisporum infection and bimolecular fluorescence complementation (BiFC) showed the RabGAP22-AGT1 protein complex to be localized in the peroxisomes. Further, fungal-induced RabGAP22 expression was found to be associated with elevated endogenous levels of the plant hormones jasmonic acid (JA) and abscisic acid (ABA). An inadequate ABA response in rabgap22-1 mutants, coupled with a stomata-localized expression of RabGAP22 and impairment of guard cell closure in response to V. longisporum and Pseudomonas syringae, suggest that RabGAP22 has multiple roles in innate immunity.Jonas RoosSarosh BejaiShinichi OideChristina DixeliusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88187 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonas Roos Sarosh Bejai Shinichi Oide Christina Dixelius RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
description	Verticillium longisporum is a soil-borne pathogen with a preference for plants within the family Brassicaceae. Following invasion of the roots, the fungus proliferates in the plant vascular system leading to stunted plant growth, chlorosis and premature senescence. RabGTPases have been demonstrated to play a crucial role in regulating multiple responses in plants. Here, we report on the identification and characterization of the Rab GTPase-activating protein RabGAP22 gene from Arabidopsis, as an activator of multiple components in the immune responses to V. longisporum. RabGAP22Pro :GUS transgenic lines showed GUS expression predominantly in root meristems, vascular tissues and stomata, whereas the RabGAP22 protein localized in the nucleus. Reduced RabGAP22 transcript levels in mutants of the brassinolide (BL) signaling gene BRI1-associated receptor kinase 1, together with a reduction of fungal proliferation following BL pretreatment, suggested RabGAP22 to be involved in BL-mediated responses. Pull-down assays revealed serine:glyoxylate aminotransferase (AGT1) as an interacting partner during V. longisporum infection and bimolecular fluorescence complementation (BiFC) showed the RabGAP22-AGT1 protein complex to be localized in the peroxisomes. Further, fungal-induced RabGAP22 expression was found to be associated with elevated endogenous levels of the plant hormones jasmonic acid (JA) and abscisic acid (ABA). An inadequate ABA response in rabgap22-1 mutants, coupled with a stomata-localized expression of RabGAP22 and impairment of guard cell closure in response to V. longisporum and Pseudomonas syringae, suggest that RabGAP22 has multiple roles in innate immunity.
format	article
author	Jonas Roos Sarosh Bejai Shinichi Oide Christina Dixelius
author_facet	Jonas Roos Sarosh Bejai Shinichi Oide Christina Dixelius
author_sort	Jonas Roos
title	RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
title_short	RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
title_full	RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
title_fullStr	RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
title_full_unstemmed	RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
title_sort	rabgap22 is required for defense to the vascular pathogen verticillium longisporum and contributes to stomata immunity.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/376c2d69b5d24fc0bac768a68ae42379
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RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.

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