Nanomedicine engulfed by macrophages for targeted tumor therapy

Siwen Li,1,* Song Feng,1,* Li Ding,1 Yuxi Liu,1 Qiuyun Zhu,1 Zhiyu Qian,2 Yueqing Gu1 1Department of Biomedical Engineering, China Pharmaceutical University, 2Department of Biomedical Engineering, School of Automation, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, People&...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li S, Feng S, Ding L, Liu Y, Zhu Q, Qian Z, Gu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	macrophage drug capacity SOC-PTX tumor targeted therapy. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3774101837534cfe83cad524715380b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3774101837534cfe83cad524715380b8
record_format	dspace
spelling	oai:doaj.org-article:3774101837534cfe83cad524715380b82021-12-02T05:36:20ZNanomedicine engulfed by macrophages for targeted tumor therapy1178-2013https://doaj.org/article/3774101837534cfe83cad524715380b82016-08-01T00:00:00Zhttps://www.dovepress.com/nanomedicine-engulfed-by-macrophages-for-targeted-tumor-therapy-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Siwen Li,1,* Song Feng,1,* Li Ding,1 Yuxi Liu,1 Qiuyun Zhu,1 Zhiyu Qian,2 Yueqing Gu1 1Department of Biomedical Engineering, China Pharmaceutical University, 2Department of Biomedical Engineering, School of Automation, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, People’s Republic of China *These authors contributed equally to this work Abstract: Macrophages, exhibiting high intrinsic accumulation and infiltration into tumor tissues, are a novel drug vehicle for directional drug delivery. However, the low drug-loading (DL) capacity and the drug cytotoxicity to the cell vehicle have limited the application of macrophages in tumor therapy. In this study, different drugs involving small molecular and nanoparticle drugs were loaded into intrinsic macrophages to find a better way to overcome these limitations. Their DL capacity and cytotoxicity to the macrophages were first compared. Furthermore, their phagocytic ratio, dynamic distributions, and tumoricidal effects were also investigated. Results indicated that more lipid-soluble molecules and DL particles can be phagocytized by macrophages than hydrophilic ones. In addition, the N-succinyl-N'-octyl chitosan (SOC) DL particles showed low cytotoxicity to the macrophage itself, while the dynamic biodistribution of macrophages engulfed with different particles/small molecules showed similar profiles, mainly excreted from liver to intestine pathway. Furthermore, macrophages loaded with SOC–paclitaxel (PTX) particles exhibited greater therapeutic efficacies than those of macrophages directly carrying small molecular drugs such as doxorubicin and PTX. Interestingly, macrophages displayed stronger targeting ability to the tumor site hypersecreting chemokine in immunocompetent mice in comparison to the tumor site secreting low levels of chemokine in immunodeficiency mice. Finally, results demonstrated that macrophages carrying SOC–PTX are a promising pharmaceutical preparation for tumor-targeted therapy. Keywords: macrophage, drug-loading capacity, SOC–PTX, tumor-targeted therapyLi SFeng SDing LLiu YZhu QQian ZGu YDove Medical Pressarticlemacrophagedrug capacitySOC-PTXtumor targeted therapy.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4107-4124 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	macrophage drug capacity SOC-PTX tumor targeted therapy. Medicine (General) R5-920
spellingShingle	macrophage drug capacity SOC-PTX tumor targeted therapy. Medicine (General) R5-920 Li S Feng S Ding L Liu Y Zhu Q Qian Z Gu Y Nanomedicine engulfed by macrophages for targeted tumor therapy
description	Siwen Li,1,* Song Feng,1,* Li Ding,1 Yuxi Liu,1 Qiuyun Zhu,1 Zhiyu Qian,2 Yueqing Gu1 1Department of Biomedical Engineering, China Pharmaceutical University, 2Department of Biomedical Engineering, School of Automation, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, People’s Republic of China *These authors contributed equally to this work Abstract: Macrophages, exhibiting high intrinsic accumulation and infiltration into tumor tissues, are a novel drug vehicle for directional drug delivery. However, the low drug-loading (DL) capacity and the drug cytotoxicity to the cell vehicle have limited the application of macrophages in tumor therapy. In this study, different drugs involving small molecular and nanoparticle drugs were loaded into intrinsic macrophages to find a better way to overcome these limitations. Their DL capacity and cytotoxicity to the macrophages were first compared. Furthermore, their phagocytic ratio, dynamic distributions, and tumoricidal effects were also investigated. Results indicated that more lipid-soluble molecules and DL particles can be phagocytized by macrophages than hydrophilic ones. In addition, the N-succinyl-N'-octyl chitosan (SOC) DL particles showed low cytotoxicity to the macrophage itself, while the dynamic biodistribution of macrophages engulfed with different particles/small molecules showed similar profiles, mainly excreted from liver to intestine pathway. Furthermore, macrophages loaded with SOC–paclitaxel (PTX) particles exhibited greater therapeutic efficacies than those of macrophages directly carrying small molecular drugs such as doxorubicin and PTX. Interestingly, macrophages displayed stronger targeting ability to the tumor site hypersecreting chemokine in immunocompetent mice in comparison to the tumor site secreting low levels of chemokine in immunodeficiency mice. Finally, results demonstrated that macrophages carrying SOC–PTX are a promising pharmaceutical preparation for tumor-targeted therapy. Keywords: macrophage, drug-loading capacity, SOC–PTX, tumor-targeted therapy
format	article
author	Li S Feng S Ding L Liu Y Zhu Q Qian Z Gu Y
author_facet	Li S Feng S Ding L Liu Y Zhu Q Qian Z Gu Y
author_sort	Li S
title	Nanomedicine engulfed by macrophages for targeted tumor therapy
title_short	Nanomedicine engulfed by macrophages for targeted tumor therapy
title_full	Nanomedicine engulfed by macrophages for targeted tumor therapy
title_fullStr	Nanomedicine engulfed by macrophages for targeted tumor therapy
title_full_unstemmed	Nanomedicine engulfed by macrophages for targeted tumor therapy
title_sort	nanomedicine engulfed by macrophages for targeted tumor therapy
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/3774101837534cfe83cad524715380b8
work_keys_str_mv	AT lis nanomedicineengulfedbymacrophagesfortargetedtumortherapy AT fengs nanomedicineengulfedbymacrophagesfortargetedtumortherapy AT dingl nanomedicineengulfedbymacrophagesfortargetedtumortherapy AT liuy nanomedicineengulfedbymacrophagesfortargetedtumortherapy AT zhuq nanomedicineengulfedbymacrophagesfortargetedtumortherapy AT qianz nanomedicineengulfedbymacrophagesfortargetedtumortherapy AT guy nanomedicineengulfedbymacrophagesfortargetedtumortherapy
_version_	1718400288837074944

Nanomedicine engulfed by macrophages for targeted tumor therapy

Ejemplares similares