Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.

The comparison of transcriptome profiles among populations is a powerful tool for investigating the role of gene expression change in adaptation to new environments. In this study, we use massively parallel sequencing of 3' cDNAs obtained from large samples of adult males, to compare a populati...

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Autores principales:	François Wurmser, Tristan Mary-Huard, Jean-Jacques Daudin, Dominique Joly, Catherine Montchamp-Moreau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3779f53149c2452d89aa7588d8a4ee52
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spelling	oai:doaj.org-article:3779f53149c2452d89aa7588d8a4ee522021-11-18T08:45:57ZVariation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.1932-620310.1371/journal.pone.0079750https://doaj.org/article/3779f53149c2452d89aa7588d8a4ee522013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260296/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The comparison of transcriptome profiles among populations is a powerful tool for investigating the role of gene expression change in adaptation to new environments. In this study, we use massively parallel sequencing of 3' cDNAs obtained from large samples of adult males, to compare a population of Drosophila simulans from a natural reserve within its ancestral range (eastern Africa) with a derived population collected in the strongly anthropized Rhône valley (France). The goal was to scan for adaptation linked to the invasion of new environments by the species. Among 15,090 genes retained for the analysis, 794 were found to be differentially expressed between the two populations. We observed an increase in expression of reproduction-related genes in eastern Africa, and an even stronger increase in expression of Cytochrome P450, Glutathione transferase and Glucuronosyl transferase genes in the derived population. These three gene families are involved in detoxification processes, which suggests that pesticides are a major environmental pressure for the species in this area. The survey of the Cyp6g1 upstream region revealed the insertion of a transposable element, Juan, in the regulatory sequence that is almost fixed in the Rhône Valley, but barely present in Mayotte. This shows that Cyp6g1 has undergone parallel evolution in derived populations of D. simulans as previously shown for D. melanogaster. The increasing amount of data produced by comparative population genomics and transcriptomics should permit the identification of additional genes associated with functional divergence among those differentially expressed.François WurmserTristan Mary-HuardJean-Jacques DaudinDominique JolyCatherine Montchamp-MoreauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79750 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q François Wurmser Tristan Mary-Huard Jean-Jacques Daudin Dominique Joly Catherine Montchamp-Moreau Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.
description	The comparison of transcriptome profiles among populations is a powerful tool for investigating the role of gene expression change in adaptation to new environments. In this study, we use massively parallel sequencing of 3' cDNAs obtained from large samples of adult males, to compare a population of Drosophila simulans from a natural reserve within its ancestral range (eastern Africa) with a derived population collected in the strongly anthropized Rhône valley (France). The goal was to scan for adaptation linked to the invasion of new environments by the species. Among 15,090 genes retained for the analysis, 794 were found to be differentially expressed between the two populations. We observed an increase in expression of reproduction-related genes in eastern Africa, and an even stronger increase in expression of Cytochrome P450, Glutathione transferase and Glucuronosyl transferase genes in the derived population. These three gene families are involved in detoxification processes, which suggests that pesticides are a major environmental pressure for the species in this area. The survey of the Cyp6g1 upstream region revealed the insertion of a transposable element, Juan, in the regulatory sequence that is almost fixed in the Rhône Valley, but barely present in Mayotte. This shows that Cyp6g1 has undergone parallel evolution in derived populations of D. simulans as previously shown for D. melanogaster. The increasing amount of data produced by comparative population genomics and transcriptomics should permit the identification of additional genes associated with functional divergence among those differentially expressed.
format	article
author	François Wurmser Tristan Mary-Huard Jean-Jacques Daudin Dominique Joly Catherine Montchamp-Moreau
author_facet	François Wurmser Tristan Mary-Huard Jean-Jacques Daudin Dominique Joly Catherine Montchamp-Moreau
author_sort	François Wurmser
title	Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.
title_short	Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.
title_full	Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.
title_fullStr	Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.
title_full_unstemmed	Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.
title_sort	variation of gene expression associated with colonisation of an anthropized environment: comparison between african and european populations of drosophila simulans.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3779f53149c2452d89aa7588d8a4ee52
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Variation of gene expression associated with colonisation of an anthropized environment: comparison between African and European populations of Drosophila simulans.

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