Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018

Abstract Background Urinary tract infections (UTIs) are common in dogs and can be caused by multidrug‐resistant Escherichia coli (E coli). Objective To describe the frequency and mechanisms of antimicrobial resistance (AMR) among E coli causing UTIs in dogs in Western Canada during a 4‐year surveill...

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Autores principales:	Rachel Courtice, Michelle Sniatynski, Joseph E. Rubin
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	bacterial gram‐negative bacteria microbiology multidrug resistant renal Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/378c65d34e7f4508bbc332820c0aac0e
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spelling	oai:doaj.org-article:378c65d34e7f4508bbc332820c0aac0e2021-11-30T17:01:03ZCharacterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 20181939-16760891-664010.1111/jvim.16103https://doaj.org/article/378c65d34e7f4508bbc332820c0aac0e2021-05-01T00:00:00Zhttps://doi.org/10.1111/jvim.16103https://doaj.org/toc/0891-6640https://doaj.org/toc/1939-1676Abstract Background Urinary tract infections (UTIs) are common in dogs and can be caused by multidrug‐resistant Escherichia coli (E coli). Objective To describe the frequency and mechanisms of antimicrobial resistance (AMR) among E coli causing UTIs in dogs in Western Canada during a 4‐year surveillance period. Animals Urine from 516 dogs. Methods From November 2014 to 2018, 516 nonduplicate E coli isolates from the urine of dogs were collected from a diagnostic laboratory. Susceptibility testing was determined for a panel of 14 antimicrobials belonging to 7 drug classes. Resistant isolates were screened for the presence of extended‐spectrum beta‐lactamases (ESBLs), AmpC β‐lactamases, and plasmid‐mediated quinolone resistance (PMQR) genes. Epidemiological relationships were assessed by MLST. Results 80.2% (414/516) of isolates were susceptible to all antimicrobials tested. There was no significant increase in the proportion of isolates resistant to any of the tested antimicrobials during the study period. Resistance to ampicillin was the most common (14.9%, 77/516). Overall, 12 isolates had blaCMY‐2‐type AmpC β‐lactamases, and 7 produced CTX‐M‐type ESBLs. A single isolate had the aac(6′)‐Ib‐cr PMQR gene. The qnr and qepA determinants were not detected. A single isolate belonging to the pandemic lineage ST131 was identified. Conclusion Escherichia coli isolated from the urine of dogs in our region remain susceptible to first‐line therapies, though resistance, particularly to the aminopenicillins, warrants monitoring. This is the first description of E coli ST131 from a companion animal in Canada.Rachel CourticeMichelle SniatynskiJoseph E. RubinWileyarticlebacterialgram‐negative bacteriamicrobiologymultidrug resistantrenalVeterinary medicineSF600-1100ENJournal of Veterinary Internal Medicine, Vol 35, Iss 3, Pp 1389-1396 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial gram‐negative bacteria microbiology multidrug resistant renal Veterinary medicine SF600-1100
spellingShingle	bacterial gram‐negative bacteria microbiology multidrug resistant renal Veterinary medicine SF600-1100 Rachel Courtice Michelle Sniatynski Joseph E. Rubin Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018
description	Abstract Background Urinary tract infections (UTIs) are common in dogs and can be caused by multidrug‐resistant Escherichia coli (E coli). Objective To describe the frequency and mechanisms of antimicrobial resistance (AMR) among E coli causing UTIs in dogs in Western Canada during a 4‐year surveillance period. Animals Urine from 516 dogs. Methods From November 2014 to 2018, 516 nonduplicate E coli isolates from the urine of dogs were collected from a diagnostic laboratory. Susceptibility testing was determined for a panel of 14 antimicrobials belonging to 7 drug classes. Resistant isolates were screened for the presence of extended‐spectrum beta‐lactamases (ESBLs), AmpC β‐lactamases, and plasmid‐mediated quinolone resistance (PMQR) genes. Epidemiological relationships were assessed by MLST. Results 80.2% (414/516) of isolates were susceptible to all antimicrobials tested. There was no significant increase in the proportion of isolates resistant to any of the tested antimicrobials during the study period. Resistance to ampicillin was the most common (14.9%, 77/516). Overall, 12 isolates had blaCMY‐2‐type AmpC β‐lactamases, and 7 produced CTX‐M‐type ESBLs. A single isolate had the aac(6′)‐Ib‐cr PMQR gene. The qnr and qepA determinants were not detected. A single isolate belonging to the pandemic lineage ST131 was identified. Conclusion Escherichia coli isolated from the urine of dogs in our region remain susceptible to first‐line therapies, though resistance, particularly to the aminopenicillins, warrants monitoring. This is the first description of E coli ST131 from a companion animal in Canada.
format	article
author	Rachel Courtice Michelle Sniatynski Joseph E. Rubin
author_facet	Rachel Courtice Michelle Sniatynski Joseph E. Rubin
author_sort	Rachel Courtice
title	Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018
title_short	Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018
title_full	Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018
title_fullStr	Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018
title_full_unstemmed	Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018
title_sort	characterization of antimicrobial‐resistant escherichia coli causing urinary tract infections in dogs: passive surveillance in saskatchewan, canada 2014 to 2018
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/378c65d34e7f4508bbc332820c0aac0e
work_keys_str_mv	AT rachelcourtice characterizationofantimicrobialresistantescherichiacolicausingurinarytractinfectionsindogspassivesurveillanceinsaskatchewancanada2014to2018 AT michellesniatynski characterizationofantimicrobialresistantescherichiacolicausingurinarytractinfectionsindogspassivesurveillanceinsaskatchewancanada2014to2018 AT josepherubin characterizationofantimicrobialresistantescherichiacolicausingurinarytractinfectionsindogspassivesurveillanceinsaskatchewancanada2014to2018
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Characterization of antimicrobial‐resistant Escherichia coli causing urinary tract infections in dogs: Passive surveillance in Saskatchewan, Canada 2014 to 2018

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