Biosynthesis of brain cytoplasmic 200 RNA

Abstract Brain cytoplasmic 200 RNA (BC200 RNA), a neuron-specific non-coding RNA, is also highly expressed in a number of tumors of non-neuronal origin. However, the biosynthesis of BC200 RNA remains poorly understood. In this study, we show that the efficient transcription of BC200 RNA requires bot...

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Autores principales:	Youngmi Kim, Jungmin Lee, Heegwon Shin, Seonghui Jang, Sun Chang Kim, Younghoon Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/378cf45926284083b8569e9d2b8003fc
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spelling	oai:doaj.org-article:378cf45926284083b8569e9d2b8003fc2021-12-02T12:30:34ZBiosynthesis of brain cytoplasmic 200 RNA10.1038/s41598-017-05097-32045-2322https://doaj.org/article/378cf45926284083b8569e9d2b8003fc2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05097-3https://doaj.org/toc/2045-2322Abstract Brain cytoplasmic 200 RNA (BC200 RNA), a neuron-specific non-coding RNA, is also highly expressed in a number of tumors of non-neuronal origin. However, the biosynthesis of BC200 RNA remains poorly understood. In this study, we show that the efficient transcription of BC200 RNA requires both internal and upstream promoter elements in cancer cells. The transcription complex seems to interact with a broad range of sequences within the upstream 100-bp region. The cellular levels and half-lives of BC200 RNA were found to differ across various cancer cell types, but there was no significant correlation between these parameters. Exogenously expressed BC200 RNA had a shorter half-life than that observed for the endogenous version in cancer cells, suggesting that BC200 RNA might be protected by some limiting factor(s) in cancer cells. Transient transfection experiments showed that the transcriptional activity of the exogenous BC200 RNA promoter element varied depending on the cancer cell type. However, the promoter activities together with the half-life data could not explain the differences in the levels of BC200 RNA among different cell types, suggesting that there is another level of transcriptional regulation beyond that detected by our transient transfection experiments.Youngmi KimJungmin LeeHeegwon ShinSeonghui JangSun Chang KimYounghoon LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Youngmi Kim Jungmin Lee Heegwon Shin Seonghui Jang Sun Chang Kim Younghoon Lee Biosynthesis of brain cytoplasmic 200 RNA
description	Abstract Brain cytoplasmic 200 RNA (BC200 RNA), a neuron-specific non-coding RNA, is also highly expressed in a number of tumors of non-neuronal origin. However, the biosynthesis of BC200 RNA remains poorly understood. In this study, we show that the efficient transcription of BC200 RNA requires both internal and upstream promoter elements in cancer cells. The transcription complex seems to interact with a broad range of sequences within the upstream 100-bp region. The cellular levels and half-lives of BC200 RNA were found to differ across various cancer cell types, but there was no significant correlation between these parameters. Exogenously expressed BC200 RNA had a shorter half-life than that observed for the endogenous version in cancer cells, suggesting that BC200 RNA might be protected by some limiting factor(s) in cancer cells. Transient transfection experiments showed that the transcriptional activity of the exogenous BC200 RNA promoter element varied depending on the cancer cell type. However, the promoter activities together with the half-life data could not explain the differences in the levels of BC200 RNA among different cell types, suggesting that there is another level of transcriptional regulation beyond that detected by our transient transfection experiments.
format	article
author	Youngmi Kim Jungmin Lee Heegwon Shin Seonghui Jang Sun Chang Kim Younghoon Lee
author_facet	Youngmi Kim Jungmin Lee Heegwon Shin Seonghui Jang Sun Chang Kim Younghoon Lee
author_sort	Youngmi Kim
title	Biosynthesis of brain cytoplasmic 200 RNA
title_short	Biosynthesis of brain cytoplasmic 200 RNA
title_full	Biosynthesis of brain cytoplasmic 200 RNA
title_fullStr	Biosynthesis of brain cytoplasmic 200 RNA
title_full_unstemmed	Biosynthesis of brain cytoplasmic 200 RNA
title_sort	biosynthesis of brain cytoplasmic 200 rna
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/378cf45926284083b8569e9d2b8003fc
work_keys_str_mv	AT youngmikim biosynthesisofbraincytoplasmic200rna AT jungminlee biosynthesisofbraincytoplasmic200rna AT heegwonshin biosynthesisofbraincytoplasmic200rna AT seonghuijang biosynthesisofbraincytoplasmic200rna AT sunchangkim biosynthesisofbraincytoplasmic200rna AT younghoonlee biosynthesisofbraincytoplasmic200rna
_version_	1718394327041835008

Biosynthesis of brain cytoplasmic 200 RNA

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