Evolution and origin of sliding clamp in bacteria, archaea and eukarya.

The replication of DNA is an essential process in all domains of life. A protein often involved in replication is the sliding clamp. The sliding clamp encircles the DNA and helps replicative polymerase stay attached to the replication machinery increasing the processivity of the polymerase. In eukar...

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Autores principales:	Sandesh Acharya, Amol Dahal, Hitesh Kumar Bhattarai
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/378ef76f545e46fe903a1b65f2babae4
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spelling	oai:doaj.org-article:378ef76f545e46fe903a1b65f2babae42021-12-02T20:15:04ZEvolution and origin of sliding clamp in bacteria, archaea and eukarya.1932-620310.1371/journal.pone.0241093https://doaj.org/article/378ef76f545e46fe903a1b65f2babae42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0241093https://doaj.org/toc/1932-6203The replication of DNA is an essential process in all domains of life. A protein often involved in replication is the sliding clamp. The sliding clamp encircles the DNA and helps replicative polymerase stay attached to the replication machinery increasing the processivity of the polymerase. In eukaryotes and archaea, the sliding clamp is called the Proliferating Cell Nuclear Antigen (PCNA) and consists of two domains. This PCNA forms a trimer encircling the DNA as a hexamer. In bacteria, the structure of the sliding clamp is highly conserved, but the protein itself, called beta clamp, contains three domains, which dimerize to form a hexamer. The bulk of literature touts a conservation of the structure of the sliding clamp, but fails to recognize the conservation of protein sequence among sliding clamps. In this paper, we have used PSI blast to the second iteration in NCBI to show a statistically significant sequence homology between Pyrococcus furiosus PCNA and Kallipyga gabonensis beta clamp. The last two domains of beta clamp align with the two domains of PCNA. This homology data demonstrates that PCNA and beta clamp arose from a common ancestor. In this paper, we have further used beta clamp and PCNA sequences from diverse bacteria, archaea and eukarya to build maximum likelihood phylogenetic tree. Most, but not all, species in different domains of life harbor one sliding clamp from vertical inheritance. Some of these species that have two or more sliding clamps have acquired them from gene duplication or horizontal gene transfer events.Sandesh AcharyaAmol DahalHitesh Kumar BhattaraiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0241093 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandesh Acharya Amol Dahal Hitesh Kumar Bhattarai Evolution and origin of sliding clamp in bacteria, archaea and eukarya.
description	The replication of DNA is an essential process in all domains of life. A protein often involved in replication is the sliding clamp. The sliding clamp encircles the DNA and helps replicative polymerase stay attached to the replication machinery increasing the processivity of the polymerase. In eukaryotes and archaea, the sliding clamp is called the Proliferating Cell Nuclear Antigen (PCNA) and consists of two domains. This PCNA forms a trimer encircling the DNA as a hexamer. In bacteria, the structure of the sliding clamp is highly conserved, but the protein itself, called beta clamp, contains three domains, which dimerize to form a hexamer. The bulk of literature touts a conservation of the structure of the sliding clamp, but fails to recognize the conservation of protein sequence among sliding clamps. In this paper, we have used PSI blast to the second iteration in NCBI to show a statistically significant sequence homology between Pyrococcus furiosus PCNA and Kallipyga gabonensis beta clamp. The last two domains of beta clamp align with the two domains of PCNA. This homology data demonstrates that PCNA and beta clamp arose from a common ancestor. In this paper, we have further used beta clamp and PCNA sequences from diverse bacteria, archaea and eukarya to build maximum likelihood phylogenetic tree. Most, but not all, species in different domains of life harbor one sliding clamp from vertical inheritance. Some of these species that have two or more sliding clamps have acquired them from gene duplication or horizontal gene transfer events.
format	article
author	Sandesh Acharya Amol Dahal Hitesh Kumar Bhattarai
author_facet	Sandesh Acharya Amol Dahal Hitesh Kumar Bhattarai
author_sort	Sandesh Acharya
title	Evolution and origin of sliding clamp in bacteria, archaea and eukarya.
title_short	Evolution and origin of sliding clamp in bacteria, archaea and eukarya.
title_full	Evolution and origin of sliding clamp in bacteria, archaea and eukarya.
title_fullStr	Evolution and origin of sliding clamp in bacteria, archaea and eukarya.
title_full_unstemmed	Evolution and origin of sliding clamp in bacteria, archaea and eukarya.
title_sort	evolution and origin of sliding clamp in bacteria, archaea and eukarya.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/378ef76f545e46fe903a1b65f2babae4
work_keys_str_mv	AT sandeshacharya evolutionandoriginofslidingclampinbacteriaarchaeaandeukarya AT amoldahal evolutionandoriginofslidingclampinbacteriaarchaeaandeukarya AT hiteshkumarbhattarai evolutionandoriginofslidingclampinbacteriaarchaeaandeukarya
_version_	1718374575309324288

Evolution and origin of sliding clamp in bacteria, archaea and eukarya.

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