Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

Abstract Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomyceta...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vratislav Peska, Petr Fajkus, Michal Bubeník, Václav Brázda, Natália Bohálová, Vojtěch Dvořáček, Jiří Fajkus, Sònia Garcia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37901a9799804d918bcb163f7d267ee4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:37901a9799804d918bcb163f7d267ee4
record_format	dspace
spelling	oai:doaj.org-article:37901a9799804d918bcb163f7d267ee42021-12-02T16:04:31ZExtraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae10.1038/s41598-021-92126-x2045-2322https://doaj.org/article/37901a9799804d918bcb163f7d267ee42021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92126-xhttps://doaj.org/toc/2045-2322Abstract Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1–10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.Vratislav PeskaPetr FajkusMichal BubeníkVáclav BrázdaNatália BohálováVojtěch DvořáčekJiří FajkusSònia GarciaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vratislav Peska Petr Fajkus Michal Bubeník Václav Brázda Natália Bohálová Vojtěch Dvořáček Jiří Fajkus Sònia Garcia Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
description	Abstract Telomerase RNA (TR) carries the template for synthesis of telomere DNA and provides a scaffold for telomerase assembly. Fungal TRs are long and have been compared to higher eukaryotes, where they show considerable diversity within phylogenetically close groups. TRs of several Saccharomycetaceae were recently identified, however, many of these remained uncharacterised in the template region. Here we show that this is mainly due to high variability in telomere sequence. We predicted the telomere sequences using Tandem Repeats Finder and then we identified corresponding putative template regions in TR candidates. Remarkably long telomere units and the corresponding putative TRs were found in Tetrapisispora species. Notably, variable lengths of the annealing sequence of the template region (1–10 nt) were found. Consequently, species with the same telomere sequence may not harbour identical TR templates. Thus, TR sequence alone can be used to predict a template region and telomere sequence, but not to determine these exactly. A conserved feature of telomere sequences, tracts of adjacent Gs, led us to test the propensity of individual telomere sequences to form G4. The results show highly diverse values of G4-propensity, indicating the lack of ubiquitous conservation of this feature across Saccharomycetaceae.
format	article
author	Vratislav Peska Petr Fajkus Michal Bubeník Václav Brázda Natália Bohálová Vojtěch Dvořáček Jiří Fajkus Sònia Garcia
author_facet	Vratislav Peska Petr Fajkus Michal Bubeník Václav Brázda Natália Bohálová Vojtěch Dvořáček Jiří Fajkus Sònia Garcia
author_sort	Vratislav Peska
title	Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
title_short	Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
title_full	Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
title_fullStr	Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
title_full_unstemmed	Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae
title_sort	extraordinary diversity of telomeres, telomerase rnas and their template regions in saccharomycetaceae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37901a9799804d918bcb163f7d267ee4
work_keys_str_mv	AT vratislavpeska extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT petrfajkus extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT michalbubenik extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT vaclavbrazda extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT nataliabohalova extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT vojtechdvoracek extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT jirifajkus extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae AT soniagarcia extraordinarydiversityoftelomerestelomerasernasandtheirtemplateregionsinsaccharomycetaceae
_version_	1718385199634448384

Extraordinary diversity of telomeres, telomerase RNAs and their template regions in Saccharomycetaceae

Ejemplares similares