Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations

Abstract Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin remains intact. In this study, we investigated drying- and temperature-induced conformational changes of nucleic acids and stallion sperm chromatin. Sperm was diluted in preservation formulation...

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Autores principales:	Raffaele Brogna, Juezhu Fan, Harald Sieme, Willem F. Wolkers, Harriëtte Oldenhof
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3799dd8fb9e44ecf930c4cf73237f882
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spelling	oai:doaj.org-article:3799dd8fb9e44ecf930c4cf73237f8822021-12-02T16:14:47ZDrying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations10.1038/s41598-021-93569-y2045-2322https://doaj.org/article/3799dd8fb9e44ecf930c4cf73237f8822021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93569-yhttps://doaj.org/toc/2045-2322Abstract Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin remains intact. In this study, we investigated drying- and temperature-induced conformational changes of nucleic acids and stallion sperm chromatin. Sperm was diluted in preservation formulations with and without sugar/albumin and subjected to convective drying at elevated temperatures on glass substrates. Accumulation of reactive oxygen species was studied during storage at different temperatures, and the sperm chromatin structure assay was used to assess DNA damage. Fourier transform infrared spectroscopy was used to identify dehydration and storage induced conformational changes in isolated DNA and sperm chromatin. Furthermore, hydrogen bonding in the preservation solutions associated with storage stability were investigated. Reactive oxygen species and DNA damage in dried sperm samples were found to accumulate with increasing storage temperature and storage duration. Non-reducing disaccharides (i.e., trehalose, sucrose) and albumin counteracted oxidative stress and preserved sperm chromatin during dried storage, whereas glucose increased DNA damage during storage. When sperm was dried in the presence of trehalose and albumin, no spectral changes were detected during storage at refrigeration temperatures, whereas under accelerated aging conditions, i.e., storage at 37 °C, spectral changes were detected indicating alterations in sperm chromatin structure.Raffaele BrognaJuezhu FanHarald SiemeWillem F. WolkersHarriëtte OldenhofNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raffaele Brogna Juezhu Fan Harald Sieme Willem F. Wolkers Harriëtte Oldenhof Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
description	Abstract Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin remains intact. In this study, we investigated drying- and temperature-induced conformational changes of nucleic acids and stallion sperm chromatin. Sperm was diluted in preservation formulations with and without sugar/albumin and subjected to convective drying at elevated temperatures on glass substrates. Accumulation of reactive oxygen species was studied during storage at different temperatures, and the sperm chromatin structure assay was used to assess DNA damage. Fourier transform infrared spectroscopy was used to identify dehydration and storage induced conformational changes in isolated DNA and sperm chromatin. Furthermore, hydrogen bonding in the preservation solutions associated with storage stability were investigated. Reactive oxygen species and DNA damage in dried sperm samples were found to accumulate with increasing storage temperature and storage duration. Non-reducing disaccharides (i.e., trehalose, sucrose) and albumin counteracted oxidative stress and preserved sperm chromatin during dried storage, whereas glucose increased DNA damage during storage. When sperm was dried in the presence of trehalose and albumin, no spectral changes were detected during storage at refrigeration temperatures, whereas under accelerated aging conditions, i.e., storage at 37 °C, spectral changes were detected indicating alterations in sperm chromatin structure.
format	article
author	Raffaele Brogna Juezhu Fan Harald Sieme Willem F. Wolkers Harriëtte Oldenhof
author_facet	Raffaele Brogna Juezhu Fan Harald Sieme Willem F. Wolkers Harriëtte Oldenhof
author_sort	Raffaele Brogna
title	Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
title_short	Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
title_full	Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
title_fullStr	Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
title_full_unstemmed	Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
title_sort	drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3799dd8fb9e44ecf930c4cf73237f882
work_keys_str_mv	AT raffaelebrogna dryingandtemperatureinducedconformationalchangesofnucleicacidsandstallionspermchromatinintrehalosepreservationformulations AT juezhufan dryingandtemperatureinducedconformationalchangesofnucleicacidsandstallionspermchromatinintrehalosepreservationformulations AT haraldsieme dryingandtemperatureinducedconformationalchangesofnucleicacidsandstallionspermchromatinintrehalosepreservationformulations AT willemfwolkers dryingandtemperatureinducedconformationalchangesofnucleicacidsandstallionspermchromatinintrehalosepreservationformulations AT harrietteoldenhof dryingandtemperatureinducedconformationalchangesofnucleicacidsandstallionspermchromatinintrehalosepreservationformulations
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Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations

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