The role of endoplasmic reticulum in in vivo cancer FDG kinetics.

A recent result obtained by means of an in vitro experiment with cancer cultured cells has configured the endoplasmic reticulum as the preferential site for the accumulation of 2-deoxy-2-[18F]fluoro-D-glucose (FDG). Such a result is coherent with cell biochemistry and is made more significant by the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sara Sommariva, Mara Scussolini, Vanessa Cossu, Cecilia Marini, Gianmario Sambuceti, Giacomo Caviglia, Michele Piana
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/379d5f385671400ba8028385289130d1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:379d5f385671400ba8028385289130d1
record_format	dspace
spelling	oai:doaj.org-article:379d5f385671400ba8028385289130d12021-12-02T20:11:09ZThe role of endoplasmic reticulum in in vivo cancer FDG kinetics.1932-620310.1371/journal.pone.0252422https://doaj.org/article/379d5f385671400ba8028385289130d12021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252422https://doaj.org/toc/1932-6203A recent result obtained by means of an in vitro experiment with cancer cultured cells has configured the endoplasmic reticulum as the preferential site for the accumulation of 2-deoxy-2-[18F]fluoro-D-glucose (FDG). Such a result is coherent with cell biochemistry and is made more significant by the fact that the reticular accumulation rate of FDG is dependent upon extracellular glucose availability. The objective of the present paper is to confirm in vivo the result obtained in vitro concerning the crucial role played by the endoplasmic reticulum in FDG cancer metabolism. This study utilizes data acquired by means of a Positron Emission Tomography scanner for small animals in the case of CT26 models of cancer tissues. The recorded concentration images are interpreted within the framework of a three-compartment model for FDG kinetics, which explicitly assumes that the endoplasmic reticulum is the dephosphorylation site for FDG in cancer cells. The numerical reduction of the compartmental model is performed by means of a regularized Gauss-Newton algorithm for numerical optimization. This analysis shows that the proposed three-compartment model equals the performance of a standard Sokoloff's two-compartment system in fitting the data. However, it provides estimates of some of the parameters, such as the phosphorylation rate of FDG, more consistent with prior biochemical information. These results are made more solid from a computational viewpoint by proving the identifiability and by performing a sensitivity analysis of the proposed compartment model.Sara SommarivaMara ScussoliniVanessa CossuCecilia MariniGianmario SambucetiGiacomo CavigliaMichele PianaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252422 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Sommariva Mara Scussolini Vanessa Cossu Cecilia Marini Gianmario Sambuceti Giacomo Caviglia Michele Piana The role of endoplasmic reticulum in in vivo cancer FDG kinetics.
description	A recent result obtained by means of an in vitro experiment with cancer cultured cells has configured the endoplasmic reticulum as the preferential site for the accumulation of 2-deoxy-2-[18F]fluoro-D-glucose (FDG). Such a result is coherent with cell biochemistry and is made more significant by the fact that the reticular accumulation rate of FDG is dependent upon extracellular glucose availability. The objective of the present paper is to confirm in vivo the result obtained in vitro concerning the crucial role played by the endoplasmic reticulum in FDG cancer metabolism. This study utilizes data acquired by means of a Positron Emission Tomography scanner for small animals in the case of CT26 models of cancer tissues. The recorded concentration images are interpreted within the framework of a three-compartment model for FDG kinetics, which explicitly assumes that the endoplasmic reticulum is the dephosphorylation site for FDG in cancer cells. The numerical reduction of the compartmental model is performed by means of a regularized Gauss-Newton algorithm for numerical optimization. This analysis shows that the proposed three-compartment model equals the performance of a standard Sokoloff's two-compartment system in fitting the data. However, it provides estimates of some of the parameters, such as the phosphorylation rate of FDG, more consistent with prior biochemical information. These results are made more solid from a computational viewpoint by proving the identifiability and by performing a sensitivity analysis of the proposed compartment model.
format	article
author	Sara Sommariva Mara Scussolini Vanessa Cossu Cecilia Marini Gianmario Sambuceti Giacomo Caviglia Michele Piana
author_facet	Sara Sommariva Mara Scussolini Vanessa Cossu Cecilia Marini Gianmario Sambuceti Giacomo Caviglia Michele Piana
author_sort	Sara Sommariva
title	The role of endoplasmic reticulum in in vivo cancer FDG kinetics.
title_short	The role of endoplasmic reticulum in in vivo cancer FDG kinetics.
title_full	The role of endoplasmic reticulum in in vivo cancer FDG kinetics.
title_fullStr	The role of endoplasmic reticulum in in vivo cancer FDG kinetics.
title_full_unstemmed	The role of endoplasmic reticulum in in vivo cancer FDG kinetics.
title_sort	role of endoplasmic reticulum in in vivo cancer fdg kinetics.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/379d5f385671400ba8028385289130d1
work_keys_str_mv	AT sarasommariva theroleofendoplasmicreticulumininvivocancerfdgkinetics AT marascussolini theroleofendoplasmicreticulumininvivocancerfdgkinetics AT vanessacossu theroleofendoplasmicreticulumininvivocancerfdgkinetics AT ceciliamarini theroleofendoplasmicreticulumininvivocancerfdgkinetics AT gianmariosambuceti theroleofendoplasmicreticulumininvivocancerfdgkinetics AT giacomocaviglia theroleofendoplasmicreticulumininvivocancerfdgkinetics AT michelepiana theroleofendoplasmicreticulumininvivocancerfdgkinetics AT sarasommariva roleofendoplasmicreticulumininvivocancerfdgkinetics AT marascussolini roleofendoplasmicreticulumininvivocancerfdgkinetics AT vanessacossu roleofendoplasmicreticulumininvivocancerfdgkinetics AT ceciliamarini roleofendoplasmicreticulumininvivocancerfdgkinetics AT gianmariosambuceti roleofendoplasmicreticulumininvivocancerfdgkinetics AT giacomocaviglia roleofendoplasmicreticulumininvivocancerfdgkinetics AT michelepiana roleofendoplasmicreticulumininvivocancerfdgkinetics
_version_	1718374937253642240

The role of endoplasmic reticulum in in vivo cancer FDG kinetics.

Ejemplares similares