Cost-Effective Multiplex Fluorescence Detection System for PCR Chip

The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera...

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Autores principales:	Sung-Hun Yun, Ji-Sung Park, Seul-Bit-Na Koo, Chan-Young Park, Yu-Seop Kim, Jong-Dae Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	real-time PCR multiplex filter wheel PCR chip point of care open platform camera Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/37a22990e53e4bca91003c316f8e85f8
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id	oai:doaj.org-article:37a22990e53e4bca91003c316f8e85f8
record_format	dspace
spelling	oai:doaj.org-article:37a22990e53e4bca91003c316f8e85f82021-11-11T18:59:35ZCost-Effective Multiplex Fluorescence Detection System for PCR Chip10.3390/s212169451424-8220https://doaj.org/article/37a22990e53e4bca91003c316f8e85f82021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/21/6945https://doaj.org/toc/1424-8220The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 μm. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.Sung-Hun YunJi-Sung ParkSeul-Bit-Na KooChan-Young ParkYu-Seop KimJong-Dae KimMDPI AGarticlereal-time PCRmultiplexfilter wheelPCR chippoint of careopen platform cameraChemical technologyTP1-1185ENSensors, Vol 21, Iss 6945, p 6945 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	real-time PCR multiplex filter wheel PCR chip point of care open platform camera Chemical technology TP1-1185
spellingShingle	real-time PCR multiplex filter wheel PCR chip point of care open platform camera Chemical technology TP1-1185 Sung-Hun Yun Ji-Sung Park Seul-Bit-Na Koo Chan-Young Park Yu-Seop Kim Jong-Dae Kim Cost-Effective Multiplex Fluorescence Detection System for PCR Chip
description	The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 μm. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.
format	article
author	Sung-Hun Yun Ji-Sung Park Seul-Bit-Na Koo Chan-Young Park Yu-Seop Kim Jong-Dae Kim
author_facet	Sung-Hun Yun Ji-Sung Park Seul-Bit-Na Koo Chan-Young Park Yu-Seop Kim Jong-Dae Kim
author_sort	Sung-Hun Yun
title	Cost-Effective Multiplex Fluorescence Detection System for PCR Chip
title_short	Cost-Effective Multiplex Fluorescence Detection System for PCR Chip
title_full	Cost-Effective Multiplex Fluorescence Detection System for PCR Chip
title_fullStr	Cost-Effective Multiplex Fluorescence Detection System for PCR Chip
title_full_unstemmed	Cost-Effective Multiplex Fluorescence Detection System for PCR Chip
title_sort	cost-effective multiplex fluorescence detection system for pcr chip
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/37a22990e53e4bca91003c316f8e85f8
work_keys_str_mv	AT sunghunyun costeffectivemultiplexfluorescencedetectionsystemforpcrchip AT jisungpark costeffectivemultiplexfluorescencedetectionsystemforpcrchip AT seulbitnakoo costeffectivemultiplexfluorescencedetectionsystemforpcrchip AT chanyoungpark costeffectivemultiplexfluorescencedetectionsystemforpcrchip AT yuseopkim costeffectivemultiplexfluorescencedetectionsystemforpcrchip AT jongdaekim costeffectivemultiplexfluorescencedetectionsystemforpcrchip
_version_	1718431636468531200

Cost-Effective Multiplex Fluorescence Detection System for PCR Chip

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