Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment

Leptin modulates insulin signaling and this involves the Akt pathway, which is influenced by changes in the inflammatory environment and with leptin regulating cytokine synthesis. We evaluated the association between activation of the insulin-signaling pathway and alterations in pro- and anti-inflam...

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Autores principales:	Vicente Barrios, Ana Campillo-Calatayud, Santiago Guerra-Cantera, Sandra Canelles, Álvaro Martín-Rivada, Laura M. Frago, Julie A. Chowen, Jesús Argente
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose tissue cytokines inflammation insulin signaling leptin liver Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/37a369bc6d914d82bc80f0162464c553
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spelling	oai:doaj.org-article:37a369bc6d914d82bc80f0162464c5532021-11-25T16:54:39ZOpposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment10.3390/biom111117342218-273Xhttps://doaj.org/article/37a369bc6d914d82bc80f0162464c5532021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1734https://doaj.org/toc/2218-273XLeptin modulates insulin signaling and this involves the Akt pathway, which is influenced by changes in the inflammatory environment and with leptin regulating cytokine synthesis. We evaluated the association between activation of the insulin-signaling pathway and alterations in pro- and anti-inflammatory cytokine levels in inguinal fat and liver of chronic central leptin infused (L), pair-fed (PF), and control rats. Signal transducer and activator of transcription 3 (STAT3) phosphorylation was increased in inguinal fat and reduced in liver of L rats. Phosphorylation of c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NFkB) was increased in inguinal fat of L rats, together with a pro-inflammatory cytokine profile, while in the liver activation of JNK and NFkB were reduced and an anti-inflammatory pattern was found. Phosphorylation of the insulin receptor, Akt and mechanistic target of rapamycin was decreased in inguinal fat and increased in liver of L rats. There was a direct relationship between pSTAT3 and JNK and a negative correlation of Akt with pSTAT3 and JNK in both tissues. These results indicate that the effects of chronically increased leptin on insulin-related signaling are tissue-specific and suggest that inflammation plays a relevant role in the crosstalk between leptin and insulin signaling.Vicente BarriosAna Campillo-CalatayudSantiago Guerra-CanteraSandra CanellesÁlvaro Martín-RivadaLaura M. FragoJulie A. ChowenJesús ArgenteMDPI AGarticleadipose tissuecytokinesinflammationinsulin signalingleptinliverMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1734, p 1734 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose tissue cytokines inflammation insulin signaling leptin liver Microbiology QR1-502
spellingShingle	adipose tissue cytokines inflammation insulin signaling leptin liver Microbiology QR1-502 Vicente Barrios Ana Campillo-Calatayud Santiago Guerra-Cantera Sandra Canelles Álvaro Martín-Rivada Laura M. Frago Julie A. Chowen Jesús Argente Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
description	Leptin modulates insulin signaling and this involves the Akt pathway, which is influenced by changes in the inflammatory environment and with leptin regulating cytokine synthesis. We evaluated the association between activation of the insulin-signaling pathway and alterations in pro- and anti-inflammatory cytokine levels in inguinal fat and liver of chronic central leptin infused (L), pair-fed (PF), and control rats. Signal transducer and activator of transcription 3 (STAT3) phosphorylation was increased in inguinal fat and reduced in liver of L rats. Phosphorylation of c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NFkB) was increased in inguinal fat of L rats, together with a pro-inflammatory cytokine profile, while in the liver activation of JNK and NFkB were reduced and an anti-inflammatory pattern was found. Phosphorylation of the insulin receptor, Akt and mechanistic target of rapamycin was decreased in inguinal fat and increased in liver of L rats. There was a direct relationship between pSTAT3 and JNK and a negative correlation of Akt with pSTAT3 and JNK in both tissues. These results indicate that the effects of chronically increased leptin on insulin-related signaling are tissue-specific and suggest that inflammation plays a relevant role in the crosstalk between leptin and insulin signaling.
format	article
author	Vicente Barrios Ana Campillo-Calatayud Santiago Guerra-Cantera Sandra Canelles Álvaro Martín-Rivada Laura M. Frago Julie A. Chowen Jesús Argente
author_facet	Vicente Barrios Ana Campillo-Calatayud Santiago Guerra-Cantera Sandra Canelles Álvaro Martín-Rivada Laura M. Frago Julie A. Chowen Jesús Argente
author_sort	Vicente Barrios
title	Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
title_short	Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
title_full	Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
title_fullStr	Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
title_full_unstemmed	Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment
title_sort	opposite effects of chronic central leptin infusion on activation of insulin signaling pathways in adipose tissue and liver are related to changes in the inflammatory environment
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/37a369bc6d914d82bc80f0162464c553
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Opposite Effects of Chronic Central Leptin Infusion on Activation of Insulin Signaling Pathways in Adipose Tissue and Liver Are Related to Changes in the Inflammatory Environment

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