A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes

Abstract Epidermal photoaging contributes to skin fragility over time and it is a risk factor for skin cancer. Photoaging has been associated for a long time with exposure to Ultraviolet-A (UVA) light, the predominant component of the solar ultraviolet radiation. While the cellular mechanisms underl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hellen Paula Valerio, Felipe Gustavo Ravagnani, Graziella Eliza Ronsein, Paolo Di Mascio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37b286dfbc544539932c587e8f29a27e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:37b286dfbc544539932c587e8f29a27e
record_format	dspace
spelling	oai:doaj.org-article:37b286dfbc544539932c587e8f29a27e2021-12-05T12:13:48ZA single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes10.1038/s41598-021-02658-52045-2322https://doaj.org/article/37b286dfbc544539932c587e8f29a27e2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02658-5https://doaj.org/toc/2045-2322Abstract Epidermal photoaging contributes to skin fragility over time and it is a risk factor for skin cancer. Photoaging has been associated for a long time with exposure to Ultraviolet-A (UVA) light, the predominant component of the solar ultraviolet radiation. While the cellular mechanisms underlying UVA-induced photoaging in the dermis have been well characterized, UVA’s action on the epidermis remains elusive. Here, proteomic analysis was conducted to derive the cellular responses induced by an environmentally relevant dose of UVA in primary human keratinocytes. We also investigated the effects of UVA on non-transformed immortalized keratinocytes (HaCaT cells), bearing potentially oncogenic mutations. We showed that UVA induces proteome remodeling and senescence in primary keratinocytes, eliciting potent antioxidant and pro-inflammatory responses. Additionally, we showed that UVA modulates the secretory phenotype of these cells to the extent of inducing paracrine oxidative stress and immune system activation in pre-malignant keratinocytes. These observations offer insights into the cellular mechanisms by which UVA drives photoaging in the skin.Hellen Paula ValerioFelipe Gustavo RavagnaniGraziella Eliza RonseinPaolo Di MascioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hellen Paula Valerio Felipe Gustavo Ravagnani Graziella Eliza Ronsein Paolo Di Mascio A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
description	Abstract Epidermal photoaging contributes to skin fragility over time and it is a risk factor for skin cancer. Photoaging has been associated for a long time with exposure to Ultraviolet-A (UVA) light, the predominant component of the solar ultraviolet radiation. While the cellular mechanisms underlying UVA-induced photoaging in the dermis have been well characterized, UVA’s action on the epidermis remains elusive. Here, proteomic analysis was conducted to derive the cellular responses induced by an environmentally relevant dose of UVA in primary human keratinocytes. We also investigated the effects of UVA on non-transformed immortalized keratinocytes (HaCaT cells), bearing potentially oncogenic mutations. We showed that UVA induces proteome remodeling and senescence in primary keratinocytes, eliciting potent antioxidant and pro-inflammatory responses. Additionally, we showed that UVA modulates the secretory phenotype of these cells to the extent of inducing paracrine oxidative stress and immune system activation in pre-malignant keratinocytes. These observations offer insights into the cellular mechanisms by which UVA drives photoaging in the skin.
format	article
author	Hellen Paula Valerio Felipe Gustavo Ravagnani Graziella Eliza Ronsein Paolo Di Mascio
author_facet	Hellen Paula Valerio Felipe Gustavo Ravagnani Graziella Eliza Ronsein Paolo Di Mascio
author_sort	Hellen Paula Valerio
title	A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
title_short	A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
title_full	A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
title_fullStr	A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
title_full_unstemmed	A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
title_sort	single dose of ultraviolet-a induces proteome remodeling and senescence in primary human keratinocytes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37b286dfbc544539932c587e8f29a27e
work_keys_str_mv	AT hellenpaulavalerio asingledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT felipegustavoravagnani asingledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT graziellaelizaronsein asingledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT paolodimascio asingledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT hellenpaulavalerio singledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT felipegustavoravagnani singledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT graziellaelizaronsein singledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes AT paolodimascio singledoseofultravioletainducesproteomeremodelingandsenescenceinprimaryhumankeratinocytes
_version_	1718372126317084672

A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes

Ejemplares similares