NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.

Epstein Barr virus (EBV) causes a highly prevalent and lifelong infection contributing to the development of some malignancies. In addition to the key role played by T cells in controlling this pathogen, NK cells mediate cytotoxicity and IFNγ production in response to EBV-infected B cells in lytic c...

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Autores principales:	Elisenda Alari-Pahissa, Michelle Ataya, Ilias Moraitis, Miriam Campos-Ruiz, Mireia Altadill, Aura Muntasell, Anna Moles, Miguel López-Botet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/37ba866f52fc4103820b1d791c8a62bf
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spelling	oai:doaj.org-article:37ba866f52fc4103820b1d791c8a62bf2021-12-02T20:00:16ZNK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.1553-73661553-737410.1371/journal.ppat.1009868https://doaj.org/article/37ba866f52fc4103820b1d791c8a62bf2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009868https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Epstein Barr virus (EBV) causes a highly prevalent and lifelong infection contributing to the development of some malignancies. In addition to the key role played by T cells in controlling this pathogen, NK cells mediate cytotoxicity and IFNγ production in response to EBV-infected B cells in lytic cycle, both directly and through antibody (Ab)-dependent activation. We recently described that EBV-specific Ab-dependent NK cell interaction with viral particles (VP) bound to B cells triggered degranulation and TNFα secretion but not B cell lysis nor IFNγ production. In this report we show that NK cell activation under these conditions reduced B cell transformation by EBV. NK cells eliminated VP from the surface of B cells through a specific and active process which required tyrosine kinase activation, actin polymerization and Ca2+, being independent of proteolysis and perforin. VP were displayed at the NK cell surface before being internalized and partially shuttled to early endosomes and lysosomes. VP transfer was encompassed by a trogocytosis process including the EBV receptor CD21, together with CD19 and CD20. Our study reveals a novel facet of the antibody-dependent NK cell mediated response to this viral infection.Elisenda Alari-PahissaMichelle AtayaIlias MoraitisMiriam Campos-RuizMireia AltadillAura MuntasellAnna MolesMiguel López-BotetPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 8, p e1009868 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Elisenda Alari-Pahissa Michelle Ataya Ilias Moraitis Miriam Campos-Ruiz Mireia Altadill Aura Muntasell Anna Moles Miguel López-Botet NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.
description	Epstein Barr virus (EBV) causes a highly prevalent and lifelong infection contributing to the development of some malignancies. In addition to the key role played by T cells in controlling this pathogen, NK cells mediate cytotoxicity and IFNγ production in response to EBV-infected B cells in lytic cycle, both directly and through antibody (Ab)-dependent activation. We recently described that EBV-specific Ab-dependent NK cell interaction with viral particles (VP) bound to B cells triggered degranulation and TNFα secretion but not B cell lysis nor IFNγ production. In this report we show that NK cell activation under these conditions reduced B cell transformation by EBV. NK cells eliminated VP from the surface of B cells through a specific and active process which required tyrosine kinase activation, actin polymerization and Ca2+, being independent of proteolysis and perforin. VP were displayed at the NK cell surface before being internalized and partially shuttled to early endosomes and lysosomes. VP transfer was encompassed by a trogocytosis process including the EBV receptor CD21, together with CD19 and CD20. Our study reveals a novel facet of the antibody-dependent NK cell mediated response to this viral infection.
format	article
author	Elisenda Alari-Pahissa Michelle Ataya Ilias Moraitis Miriam Campos-Ruiz Mireia Altadill Aura Muntasell Anna Moles Miguel López-Botet
author_facet	Elisenda Alari-Pahissa Michelle Ataya Ilias Moraitis Miriam Campos-Ruiz Mireia Altadill Aura Muntasell Anna Moles Miguel López-Botet
author_sort	Elisenda Alari-Pahissa
title	NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.
title_short	NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.
title_full	NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.
title_fullStr	NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.
title_full_unstemmed	NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.
title_sort	nk cells eliminate epstein-barr virus bound to b cells through a specific antibody-mediated uptake.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/37ba866f52fc4103820b1d791c8a62bf
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NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake.

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