LoxTnSeq: random transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
Summary The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are guided by transposon mutagenesis studies, coupled to deepsequencing (TnSeq) to identify i...
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| Main Authors: | , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Wiley
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/37be770e951f4fe5b809c00854c92a1a |
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