The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models

Abstract Although the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the...

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Autores principales:	Natalia Janik-Olchawa, Agnieszka Drozdz, Damian Ryszawy, Maciej Pudelek, Karolina Planeta, Zuzanna Setkowicz, Maciej Sniegocki, Magdalena Wytrwal-Sarna, Marta Gajewska, Joanna Chwiej
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37c29b6fdfce467189d0cbec068c8910
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spelling	oai:doaj.org-article:37c29b6fdfce467189d0cbec068c89102021-11-14T12:19:59ZThe influence of IONPs core size on their biocompatibility and activity in in vitro cellular models10.1038/s41598-021-01237-y2045-2322https://doaj.org/article/37c29b6fdfce467189d0cbec068c89102021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01237-yhttps://doaj.org/toc/2045-2322Abstract Although the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the influence of PEG-coated-magnetite NPs with the core of 5, 10 and 30 nm on six carefully selected cell lines. The proliferation rate, viability, metabolic activity, migration activity, ROS levels and cytoskeleton architecture of cells have been evaluated for specified incubation periods. These were 24 and 72-h long incubations with IONPs administered in two doses: 5 and 25 µg Fe/ml. A decrease in viability was observed after exposure to the tested NPs for all the analyzed cell lines. This effect was not connected with core diameter but depended on the exposure time to the nanomaterials. IONPs increased not only the proliferation rate of macrophages—being phagocytic cells—but also, under certain conditions stimulated tumor cell divisions. Most likely, the increase in proliferation rate of macrophages contributed to the changes in the architecture of their cytoskeleton. The growth in the level of ROS in cells had been induced mainly by the smallest NPs. This effect was observed for HEK293T cells and two cancerous lines: U87MG (at both doses tested) and T98G (only for the higher dose). This requires further study concerning both potential toxicity of such IONPs to the kidneys and assessing their therapeutic potential in the treatment of glioblastoma multiforme.Natalia Janik-OlchawaAgnieszka DrozdzDamian RyszawyMaciej PudelekKarolina PlanetaZuzanna SetkowiczMaciej SniegockiMagdalena Wytrwal-SarnaMarta GajewskaJoanna ChwiejNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalia Janik-Olchawa Agnieszka Drozdz Damian Ryszawy Maciej Pudelek Karolina Planeta Zuzanna Setkowicz Maciej Sniegocki Magdalena Wytrwal-Sarna Marta Gajewska Joanna Chwiej The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
description	Abstract Although the key factor affecting the biocompatibility of IONPs is the core size, there is a lack of regular investigation concerning the impact of the parameter on the toxicity of these nanomaterials. Therefore, such studies were carried out in this paper. Their purpose was to compare the influence of PEG-coated-magnetite NPs with the core of 5, 10 and 30 nm on six carefully selected cell lines. The proliferation rate, viability, metabolic activity, migration activity, ROS levels and cytoskeleton architecture of cells have been evaluated for specified incubation periods. These were 24 and 72-h long incubations with IONPs administered in two doses: 5 and 25 µg Fe/ml. A decrease in viability was observed after exposure to the tested NPs for all the analyzed cell lines. This effect was not connected with core diameter but depended on the exposure time to the nanomaterials. IONPs increased not only the proliferation rate of macrophages—being phagocytic cells—but also, under certain conditions stimulated tumor cell divisions. Most likely, the increase in proliferation rate of macrophages contributed to the changes in the architecture of their cytoskeleton. The growth in the level of ROS in cells had been induced mainly by the smallest NPs. This effect was observed for HEK293T cells and two cancerous lines: U87MG (at both doses tested) and T98G (only for the higher dose). This requires further study concerning both potential toxicity of such IONPs to the kidneys and assessing their therapeutic potential in the treatment of glioblastoma multiforme.
format	article
author	Natalia Janik-Olchawa Agnieszka Drozdz Damian Ryszawy Maciej Pudelek Karolina Planeta Zuzanna Setkowicz Maciej Sniegocki Magdalena Wytrwal-Sarna Marta Gajewska Joanna Chwiej
author_facet	Natalia Janik-Olchawa Agnieszka Drozdz Damian Ryszawy Maciej Pudelek Karolina Planeta Zuzanna Setkowicz Maciej Sniegocki Magdalena Wytrwal-Sarna Marta Gajewska Joanna Chwiej
author_sort	Natalia Janik-Olchawa
title	The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
title_short	The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
title_full	The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
title_fullStr	The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
title_full_unstemmed	The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models
title_sort	influence of ionps core size on their biocompatibility and activity in in vitro cellular models
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37c29b6fdfce467189d0cbec068c8910
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The influence of IONPs core size on their biocompatibility and activity in in vitro cellular models

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