Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance

Abstract The aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 × Catimor UFV 1602-215) previously identified as tolerant in field conditions. F...

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Autores principales:	Meline de Oliveira Santos, Larissa Sousa Coelho, Gladyston Rodrigues Carvalho, Cesar Elias Botelho, Luana Ferreira Torres, Diego Júnior Martins Vilela, Alan Carvalho Andrade, Vânia Aparecida Silva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37cff46b145f4f47a3d437b8c049fcac
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spelling	oai:doaj.org-article:37cff46b145f4f47a3d437b8c049fcac2021-12-02T18:17:41ZPhotochemical efficiency correlated with candidate gene expression promote coffee drought tolerance10.1038/s41598-021-86689-y2045-2322https://doaj.org/article/37cff46b145f4f47a3d437b8c049fcac2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86689-yhttps://doaj.org/toc/2045-2322Abstract The aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 × Catimor UFV 1602-215) previously identified as tolerant in field conditions. Four progenies (2, 5, 12 and 15) were evaluated under water-deficit conditions (water deficit imposed 8 months after transplanting seedlings to the pots) and under irrigated system. Evaluations of physiological parameters and expression of candidate genes for drought tolerance were performed. Progeny 5 showed capacity to maintain water potential, which contributed to lower qP variation between irrigated and deficit conditions. However, the increases of qN and NPQ in response to stress indicate that this progeny is photochemically responsive to small variations of Ψam protecting the photosystem and maintaining qP. Data obtained for progeny 12 indicated a lower water status maintenance capacity, but with increased qN and NPQ providing maintenance of the ɸPSII and ETR parameters. A PCA analysis revealed that the genes coding regulatory proteins, ABA-synthesis, cellular protectors, isoforms of ascorbate peroxidase clearly displayed a major response to drought stress and discriminated the progenies 5 and 12 which showed a better photochemical response. The genes CaMYB1, CaERF017, CaEDR2, CaNCED, CaAPX1, CaAPX5, CaGolS3, CaDHN1 and CaPYL8a were up-regulated in the arabica coffee progenies with greater photochemical efficiency under deficit and therefore contributing to efficiency of the photosynthesis in drought tolerant progenies.Meline de Oliveira SantosLarissa Sousa CoelhoGladyston Rodrigues CarvalhoCesar Elias BotelhoLuana Ferreira TorresDiego Júnior Martins VilelaAlan Carvalho AndradeVânia Aparecida SilvaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meline de Oliveira Santos Larissa Sousa Coelho Gladyston Rodrigues Carvalho Cesar Elias Botelho Luana Ferreira Torres Diego Júnior Martins Vilela Alan Carvalho Andrade Vânia Aparecida Silva Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
description	Abstract The aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 × Catimor UFV 1602-215) previously identified as tolerant in field conditions. Four progenies (2, 5, 12 and 15) were evaluated under water-deficit conditions (water deficit imposed 8 months after transplanting seedlings to the pots) and under irrigated system. Evaluations of physiological parameters and expression of candidate genes for drought tolerance were performed. Progeny 5 showed capacity to maintain water potential, which contributed to lower qP variation between irrigated and deficit conditions. However, the increases of qN and NPQ in response to stress indicate that this progeny is photochemically responsive to small variations of Ψam protecting the photosystem and maintaining qP. Data obtained for progeny 12 indicated a lower water status maintenance capacity, but with increased qN and NPQ providing maintenance of the ɸPSII and ETR parameters. A PCA analysis revealed that the genes coding regulatory proteins, ABA-synthesis, cellular protectors, isoforms of ascorbate peroxidase clearly displayed a major response to drought stress and discriminated the progenies 5 and 12 which showed a better photochemical response. The genes CaMYB1, CaERF017, CaEDR2, CaNCED, CaAPX1, CaAPX5, CaGolS3, CaDHN1 and CaPYL8a were up-regulated in the arabica coffee progenies with greater photochemical efficiency under deficit and therefore contributing to efficiency of the photosynthesis in drought tolerant progenies.
format	article
author	Meline de Oliveira Santos Larissa Sousa Coelho Gladyston Rodrigues Carvalho Cesar Elias Botelho Luana Ferreira Torres Diego Júnior Martins Vilela Alan Carvalho Andrade Vânia Aparecida Silva
author_facet	Meline de Oliveira Santos Larissa Sousa Coelho Gladyston Rodrigues Carvalho Cesar Elias Botelho Luana Ferreira Torres Diego Júnior Martins Vilela Alan Carvalho Andrade Vânia Aparecida Silva
author_sort	Meline de Oliveira Santos
title	Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
title_short	Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
title_full	Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
title_fullStr	Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
title_full_unstemmed	Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
title_sort	photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37cff46b145f4f47a3d437b8c049fcac
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_version_	1718378267061256192

Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance

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