Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration

Abstract Ectopic xenografting of testis is a feasible option for preservation of male fertility and angiogenesis plays a pivotal role in xenograft survival and functionality. When compared to immature testis, the adult testis is unable to establish functional xenografts due to potentially lower effi...

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Autores principales:	Lalitha Devi, Lavanya Pothana, Sandeep Goel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37d46890682b473d846a6f02abaf4c97
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spelling	oai:doaj.org-article:37d46890682b473d846a6f02abaf4c972021-12-02T15:05:50ZDysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration10.1038/s41598-017-02604-42045-2322https://doaj.org/article/37d46890682b473d846a6f02abaf4c972017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02604-4https://doaj.org/toc/2045-2322Abstract Ectopic xenografting of testis is a feasible option for preservation of male fertility and angiogenesis plays a pivotal role in xenograft survival and functionality. When compared to immature testis, the adult testis is unable to establish functional xenografts due to potentially lower efficiency to induce angiogenesis. The precise molecular mechanism, however, remains elusive. In the present study, we compared adult and immature testis xenografts for survival, maturation and germ cell differentiation. Further, we evaluated differential expression of angiogenesis signalling-specific proteins in adult and immature testis and their xenografts. Results showed that adult testis xenografts degenerated whereas immature testis xenografts survived and established spermatogenesis with the production of haploid germ cells. Protein expression analysis demonstrated that immature testis xenografts were able to establish angiogenesis either through eNOS activation via VEGF and PI3K/AKT or through EGFR-mediated STAT3 pathway. The role of ERK/MAPK pathway in xenograft angiogenesis was ruled out. The absence or reduced expression of angiogenesis-specific proteins in adult testis and its xenografts possibly resulted in poor angiogenesis and in their subsequent degeneration. This study provides insight into angiogenesis mechanism that can be utilized to augment testis xenografting efficiency.Lalitha DeviLavanya PothanaSandeep GoelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lalitha Devi Lavanya Pothana Sandeep Goel Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
description	Abstract Ectopic xenografting of testis is a feasible option for preservation of male fertility and angiogenesis plays a pivotal role in xenograft survival and functionality. When compared to immature testis, the adult testis is unable to establish functional xenografts due to potentially lower efficiency to induce angiogenesis. The precise molecular mechanism, however, remains elusive. In the present study, we compared adult and immature testis xenografts for survival, maturation and germ cell differentiation. Further, we evaluated differential expression of angiogenesis signalling-specific proteins in adult and immature testis and their xenografts. Results showed that adult testis xenografts degenerated whereas immature testis xenografts survived and established spermatogenesis with the production of haploid germ cells. Protein expression analysis demonstrated that immature testis xenografts were able to establish angiogenesis either through eNOS activation via VEGF and PI3K/AKT or through EGFR-mediated STAT3 pathway. The role of ERK/MAPK pathway in xenograft angiogenesis was ruled out. The absence or reduced expression of angiogenesis-specific proteins in adult testis and its xenografts possibly resulted in poor angiogenesis and in their subsequent degeneration. This study provides insight into angiogenesis mechanism that can be utilized to augment testis xenografting efficiency.
format	article
author	Lalitha Devi Lavanya Pothana Sandeep Goel
author_facet	Lalitha Devi Lavanya Pothana Sandeep Goel
author_sort	Lalitha Devi
title	Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
title_short	Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
title_full	Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
title_fullStr	Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
title_full_unstemmed	Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
title_sort	dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/37d46890682b473d846a6f02abaf4c97
work_keys_str_mv	AT lalithadevi dysregulationofangiogenesisspecificsignallinginadulttestisresultsinxenograftdegeneration AT lavanyapothana dysregulationofangiogenesisspecificsignallinginadulttestisresultsinxenograftdegeneration AT sandeepgoel dysregulationofangiogenesisspecificsignallinginadulttestisresultsinxenograftdegeneration
_version_	1718388696874483712

Dysregulation of angiogenesis-specific signalling in adult testis results in xenograft degeneration

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