Oxidative damage parameters in renal tissues of aged and young rats based on gender

Duygu Uzun,1 Gülcan Güntas Korkmaz,2 Mustafa Erinç Sitar,3 Tamer Cebe,4 Karolin Yanar,3 Ufuk Çakatay,3 Seval Aydin3 1Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey; 2Kirklareli University, School of Health, Kirklareli, Turkey; 3Istanbul Un...

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Autores principales:	Uzun D, Korkmaz GG, Sitar ME, Cebe T, Yanar K, Cakatay U, Aydın S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Lipid Peroxidation Protein Oxidation Radicals Renal Aging. Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/37d6bdd67d0a4a978624acf3bde9ff1b
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spelling	oai:doaj.org-article:37d6bdd67d0a4a978624acf3bde9ff1b2021-12-02T06:38:51ZOxidative damage parameters in renal tissues of aged and young rats based on gender1178-1998https://doaj.org/article/37d6bdd67d0a4a978624acf3bde9ff1b2013-06-01T00:00:00Zhttps://www.dovepress.com/oxidative-damage-parameters-in-renal-tissues-of-aged-and-young-rats-ba-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Duygu Uzun,1 Gülcan Güntas Korkmaz,2 Mustafa Erinç Sitar,3 Tamer Cebe,4 Karolin Yanar,3 Ufuk Çakatay,3 Seval Aydin3 1Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey; 2Kirklareli University, School of Health, Kirklareli, Turkey; 3Istanbul University, Cerrahpasa Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey; 4Istanbul University Cerrahpasa Faculty of Medicine, Istanbul, Turkey Purpose: Aging is characterized by a gradual functional decrease of all systems including the kidneys. Growing evidence links altered lipid protein redox-homeostasis with renal dysfunction. The effect of sexual dimorphism on the lipid protein redox-homeostasis mechanisms in the aging kidney is obscure. In the current study, we aimed to investigate redox homeostasis as it related to sexual dimorphism on protein oxidation and lipid peroxidation parameters, as protein carbonyl (PCO), total thiol (T-SH), advanced oxidation protein products (AOPP), malondialdehyde, glutathione (GSH), and superoxide dismutase (SOD) activity, as potential aging biomarkers, which may contribute to an analysis of the free radical theory of aging. Materials and methods: The study was carried out with 16 naturally aged rats (24 months old; eight males and eight females) and their corresponding young rat groups as controls (6 months old; eight males and eight females). All of the aforementioned parameters (PCO, T-SH, AOPP, MDA, GSH, SOD) were measured manually instead of automated devices or ELISA kits. Results: PCO, AOPP, and malondialdehyde levels in aged rats were significantly higher in the older rat group than in the younger rat group, whereas SOD activities were significantly lower in old rats. T-SH levels were not significantly different in male groups; however, T-SH levels were lower in the aged female group than in the young female control group. In addition, GSH levels were significantly different between the aged rat group and the corresponding young control group for both genders. Conclusion: With respect to PCO and AOPP, impaired redox homeostasis is substantially more prominent in males than females. The decrease of G-SH levels in male groups could be attributed to stabilizing the redox status of protein thiol groups by the depletion of the GSH groups. Considering the results, the renal tissue proteins and lipids in different genders may have different susceptibilities to oxidative damage. Keywords: lipid peroxidation, protein oxidation, radicals, renal agingUzun DKorkmaz GGSitar MECebe TYanar KCakatay UAydın SDove Medical PressarticleLipid PeroxidationProtein OxidationRadicalsRenal Aging.GeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 8, Pp 809-815 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lipid Peroxidation Protein Oxidation Radicals Renal Aging. Geriatrics RC952-954.6
spellingShingle	Lipid Peroxidation Protein Oxidation Radicals Renal Aging. Geriatrics RC952-954.6 Uzun D Korkmaz GG Sitar ME Cebe T Yanar K Cakatay U Aydın S Oxidative damage parameters in renal tissues of aged and young rats based on gender
description	Duygu Uzun,1 Gülcan Güntas Korkmaz,2 Mustafa Erinç Sitar,3 Tamer Cebe,4 Karolin Yanar,3 Ufuk Çakatay,3 Seval Aydin3 1Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey; 2Kirklareli University, School of Health, Kirklareli, Turkey; 3Istanbul University, Cerrahpasa Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey; 4Istanbul University Cerrahpasa Faculty of Medicine, Istanbul, Turkey Purpose: Aging is characterized by a gradual functional decrease of all systems including the kidneys. Growing evidence links altered lipid protein redox-homeostasis with renal dysfunction. The effect of sexual dimorphism on the lipid protein redox-homeostasis mechanisms in the aging kidney is obscure. In the current study, we aimed to investigate redox homeostasis as it related to sexual dimorphism on protein oxidation and lipid peroxidation parameters, as protein carbonyl (PCO), total thiol (T-SH), advanced oxidation protein products (AOPP), malondialdehyde, glutathione (GSH), and superoxide dismutase (SOD) activity, as potential aging biomarkers, which may contribute to an analysis of the free radical theory of aging. Materials and methods: The study was carried out with 16 naturally aged rats (24 months old; eight males and eight females) and their corresponding young rat groups as controls (6 months old; eight males and eight females). All of the aforementioned parameters (PCO, T-SH, AOPP, MDA, GSH, SOD) were measured manually instead of automated devices or ELISA kits. Results: PCO, AOPP, and malondialdehyde levels in aged rats were significantly higher in the older rat group than in the younger rat group, whereas SOD activities were significantly lower in old rats. T-SH levels were not significantly different in male groups; however, T-SH levels were lower in the aged female group than in the young female control group. In addition, GSH levels were significantly different between the aged rat group and the corresponding young control group for both genders. Conclusion: With respect to PCO and AOPP, impaired redox homeostasis is substantially more prominent in males than females. The decrease of G-SH levels in male groups could be attributed to stabilizing the redox status of protein thiol groups by the depletion of the GSH groups. Considering the results, the renal tissue proteins and lipids in different genders may have different susceptibilities to oxidative damage. Keywords: lipid peroxidation, protein oxidation, radicals, renal aging
format	article
author	Uzun D Korkmaz GG Sitar ME Cebe T Yanar K Cakatay U Aydın S
author_facet	Uzun D Korkmaz GG Sitar ME Cebe T Yanar K Cakatay U Aydın S
author_sort	Uzun D
title	Oxidative damage parameters in renal tissues of aged and young rats based on gender
title_short	Oxidative damage parameters in renal tissues of aged and young rats based on gender
title_full	Oxidative damage parameters in renal tissues of aged and young rats based on gender
title_fullStr	Oxidative damage parameters in renal tissues of aged and young rats based on gender
title_full_unstemmed	Oxidative damage parameters in renal tissues of aged and young rats based on gender
title_sort	oxidative damage parameters in renal tissues of aged and young rats based on gender
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/37d6bdd67d0a4a978624acf3bde9ff1b
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Oxidative damage parameters in renal tissues of aged and young rats based on gender

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