Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans

Tri-n-butyl phosphate (TnBP), a typical alkyl organophosphate ester is widely used as an emerging flame retardant for polybrominated diphenyl ethers alternatives, but the potential toxicity and mechanism are unclear. In this study, the reproductive toxicity of TnBP and its related mechanisms were ex...

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Autores principales:	Hongdan Zhang, Tongtong Liu, Xuelong Song, Qinyu Zhou, Jielin Tang, Qianyu Sun, Yuepu Pu, Lihong Yin, Juan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	TnBP Reproductive toxicity Apoptosis DNA damage Oxidative stress, Caenorhabditis elegans Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/37db0be640774444842b4251a7afc803
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spelling	oai:doaj.org-article:37db0be640774444842b4251a7afc8032021-11-06T04:15:44ZStudy on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans0147-651310.1016/j.ecoenv.2021.112896https://doaj.org/article/37db0be640774444842b4251a7afc8032021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010083https://doaj.org/toc/0147-6513Tri-n-butyl phosphate (TnBP), a typical alkyl organophosphate ester is widely used as an emerging flame retardant for polybrominated diphenyl ethers alternatives, but the potential toxicity and mechanism are unclear. In this study, the reproductive toxicity of TnBP and its related mechanisms were explored using the Caenorhabditis elegans (C. elegans) model. After TnBP (100–1000 μg/L) exposure, brood size and the number of fertilized eggs in the uterus in C. elegans were significantly reduced, the relative area of gonad arm and the number of total germline cells in C. elegans were significantly reduced, germ cell apoptosis and germ cell DNA damage in C. elegans were significantly increased, the level of ROS in C. elegans was significantly increased. Furthermore, TnBP exposure caused abnormal gene expressions of cell apoptosis (ced-9, ced-4 and ced-3), DNA damage (hus-1, clk-2, cep-1 and egl-1) and oxidative stress (mev-1 and gas-1). TnBP exposure can lead to reproductive ability decreased and gonad development impaired in C. elegans, the mechanism of TnBP reduced reproductive ability may be related to germ cell apoptosis, germ cell DNA damage and oxidative stress. Environmental exposure to TnBP may have potential reproductive toxicity.Hongdan ZhangTongtong LiuXuelong SongQinyu ZhouJielin TangQianyu SunYuepu PuLihong YinJuan ZhangElsevierarticleTnBPReproductive toxicityApoptosisDNA damageOxidative stress, Caenorhabditis elegansEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112896- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TnBP Reproductive toxicity Apoptosis DNA damage Oxidative stress, Caenorhabditis elegans Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	TnBP Reproductive toxicity Apoptosis DNA damage Oxidative stress, Caenorhabditis elegans Environmental pollution TD172-193.5 Environmental sciences GE1-350 Hongdan Zhang Tongtong Liu Xuelong Song Qinyu Zhou Jielin Tang Qianyu Sun Yuepu Pu Lihong Yin Juan Zhang Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans
description	Tri-n-butyl phosphate (TnBP), a typical alkyl organophosphate ester is widely used as an emerging flame retardant for polybrominated diphenyl ethers alternatives, but the potential toxicity and mechanism are unclear. In this study, the reproductive toxicity of TnBP and its related mechanisms were explored using the Caenorhabditis elegans (C. elegans) model. After TnBP (100–1000 μg/L) exposure, brood size and the number of fertilized eggs in the uterus in C. elegans were significantly reduced, the relative area of gonad arm and the number of total germline cells in C. elegans were significantly reduced, germ cell apoptosis and germ cell DNA damage in C. elegans were significantly increased, the level of ROS in C. elegans was significantly increased. Furthermore, TnBP exposure caused abnormal gene expressions of cell apoptosis (ced-9, ced-4 and ced-3), DNA damage (hus-1, clk-2, cep-1 and egl-1) and oxidative stress (mev-1 and gas-1). TnBP exposure can lead to reproductive ability decreased and gonad development impaired in C. elegans, the mechanism of TnBP reduced reproductive ability may be related to germ cell apoptosis, germ cell DNA damage and oxidative stress. Environmental exposure to TnBP may have potential reproductive toxicity.
format	article
author	Hongdan Zhang Tongtong Liu Xuelong Song Qinyu Zhou Jielin Tang Qianyu Sun Yuepu Pu Lihong Yin Juan Zhang
author_facet	Hongdan Zhang Tongtong Liu Xuelong Song Qinyu Zhou Jielin Tang Qianyu Sun Yuepu Pu Lihong Yin Juan Zhang
author_sort	Hongdan Zhang
title	Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans
title_short	Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans
title_full	Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans
title_fullStr	Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans
title_full_unstemmed	Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans
title_sort	study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (tnbp) in caenorhabditis elegans
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/37db0be640774444842b4251a7afc803
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Study on the reproductive toxicity and mechanism of tri-n-butyl phosphate (TnBP) in Caenorhabditis elegans

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