Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition

Abstract Disulfides from Allium stipitatum, commonly known as Persian shallot, were previously reported to possess antibacterial properties. Analogues of these compounds, produced by S-methylthiolation of appropriate thiols using S-methyl methanethiosulfonate, exhibited antimicrobial activity, with...

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Autores principales:	Cynthia A. Danquah, Eleftheria Kakagianni, Proma Khondkar, Arundhati Maitra, Mukhlesur Rahman, Dimitrios Evangelopoulos, Timothy D. McHugh, Paul Stapleton, John Malkinson, Sanjib Bhakta, Simon Gibbons
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37e1ab0ec36449f69d577c41c3e6f546
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spelling	oai:doaj.org-article:37e1ab0ec36449f69d577c41c3e6f5462021-12-02T15:07:50ZAnalogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition10.1038/s41598-017-18948-w2045-2322https://doaj.org/article/37e1ab0ec36449f69d577c41c3e6f5462018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18948-whttps://doaj.org/toc/2045-2322Abstract Disulfides from Allium stipitatum, commonly known as Persian shallot, were previously reported to possess antibacterial properties. Analogues of these compounds, produced by S-methylthiolation of appropriate thiols using S-methyl methanethiosulfonate, exhibited antimicrobial activity, with one compound inhibiting the growth of Mycobacterium tuberculosis at 17 µM (4 mg L−1) and other compounds inhibiting Escherichia coli and multi-drug-resistant (MDR) Staphylococcus aureus at concentrations ranging between 32–138 µM (8–32 mg L−1). These compounds also displayed moderate inhibitory effects on Klebsiella and Proteus species. Whole-cell phenotypic bioassays such as the spot-culture growth inhibition assay (SPOTi), drug efflux inhibition, biofilm inhibition and cytotoxicity assays were used to evaluate these compounds. Of particular note was their ability to inhibit mycobacterial drug efflux and biofilm formation, while maintaining a high selectivity towards M. tuberculosis H37Rv. These results suggest that methyl disulfides are novel scaffolds which could lead to the development of new drugs against tuberculosis (TB).Cynthia A. DanquahEleftheria KakagianniProma KhondkarArundhati MaitraMukhlesur RahmanDimitrios EvangelopoulosTimothy D. McHughPaul StapletonJohn MalkinsonSanjib BhaktaSimon GibbonsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cynthia A. Danquah Eleftheria Kakagianni Proma Khondkar Arundhati Maitra Mukhlesur Rahman Dimitrios Evangelopoulos Timothy D. McHugh Paul Stapleton John Malkinson Sanjib Bhakta Simon Gibbons Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition
description	Abstract Disulfides from Allium stipitatum, commonly known as Persian shallot, were previously reported to possess antibacterial properties. Analogues of these compounds, produced by S-methylthiolation of appropriate thiols using S-methyl methanethiosulfonate, exhibited antimicrobial activity, with one compound inhibiting the growth of Mycobacterium tuberculosis at 17 µM (4 mg L−1) and other compounds inhibiting Escherichia coli and multi-drug-resistant (MDR) Staphylococcus aureus at concentrations ranging between 32–138 µM (8–32 mg L−1). These compounds also displayed moderate inhibitory effects on Klebsiella and Proteus species. Whole-cell phenotypic bioassays such as the spot-culture growth inhibition assay (SPOTi), drug efflux inhibition, biofilm inhibition and cytotoxicity assays were used to evaluate these compounds. Of particular note was their ability to inhibit mycobacterial drug efflux and biofilm formation, while maintaining a high selectivity towards M. tuberculosis H37Rv. These results suggest that methyl disulfides are novel scaffolds which could lead to the development of new drugs against tuberculosis (TB).
format	article
author	Cynthia A. Danquah Eleftheria Kakagianni Proma Khondkar Arundhati Maitra Mukhlesur Rahman Dimitrios Evangelopoulos Timothy D. McHugh Paul Stapleton John Malkinson Sanjib Bhakta Simon Gibbons
author_facet	Cynthia A. Danquah Eleftheria Kakagianni Proma Khondkar Arundhati Maitra Mukhlesur Rahman Dimitrios Evangelopoulos Timothy D. McHugh Paul Stapleton John Malkinson Sanjib Bhakta Simon Gibbons
author_sort	Cynthia A. Danquah
title	Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition
title_short	Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition
title_full	Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition
title_fullStr	Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition
title_full_unstemmed	Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition
title_sort	analogues of disulfides from allium stipitatum demonstrate potent anti-tubercular activities through drug efflux pump and biofilm inhibition
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/37e1ab0ec36449f69d577c41c3e6f546
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Analogues of Disulfides from Allium stipitatum Demonstrate Potent Anti-tubercular Activities through Drug Efflux Pump and Biofilm Inhibition

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