In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size

Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the com...

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Autores principales:	A A Waman, P Bohra, R Karthika Devi, J Pixy
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2021
Materias:	ex vitro rooting glucose hardening inoculum size mango ginger tropical Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/37e45cbc1d3641d58d83a3cce119d725
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spelling	oai:doaj.org-article:37e45cbc1d3641d58d83a3cce119d7252021-12-02T19:45:41ZIn vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size0973-354X2582-489910.24154/JHS.2021.v16i01.008https://doaj.org/article/37e45cbc1d3641d58d83a3cce119d7252021-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/1101https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the commonly used propagules for the species, which are also the economic part of the crop. Huge quantity of seed rhizomes is required to promote this crop in larger areas. An efficient in vitro multiplication protocol is one of the options to meet the planting material requirement. Effects of carbon source (glucose, fructose and sucrose) and concentration (1 and 3%, w/v), cytokinins (BAP and meta topolin) and concentration (1 mg/L and 2 mg/L), size of explants (one/ two/ three bud) and IBA treatment (0, 250, 500 and 1,000 mg/L) for concurrent ex vitro rooting cum hardening were studied. Results revealed that for facilitating efficient multiplication, the medium should be supplemented with glucose (3%) as a carbon source and meta topolin (1 mg/L) as cytokinin. Two-bud explant should be used for subculture as it promoted superior shoot proliferation. Concurrent ex vitro rooting cum hardening was possible even without auxin treatment. The present protocol could be useful for large-scale production of quality planting material of this underexploited tropical species.A A WamanP BohraR Karthika DeviJ PixySociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleex vitro rootingglucosehardeninginoculum sizemango gingertropicalPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 16, Iss 1, Pp 69-76 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ex vitro rooting glucose hardening inoculum size mango ginger tropical Plant culture SB1-1110
spellingShingle	ex vitro rooting glucose hardening inoculum size mango ginger tropical Plant culture SB1-1110 A A Waman P Bohra R Karthika Devi J Pixy In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size
description	Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the commonly used propagules for the species, which are also the economic part of the crop. Huge quantity of seed rhizomes is required to promote this crop in larger areas. An efficient in vitro multiplication protocol is one of the options to meet the planting material requirement. Effects of carbon source (glucose, fructose and sucrose) and concentration (1 and 3%, w/v), cytokinins (BAP and meta topolin) and concentration (1 mg/L and 2 mg/L), size of explants (one/ two/ three bud) and IBA treatment (0, 250, 500 and 1,000 mg/L) for concurrent ex vitro rooting cum hardening were studied. Results revealed that for facilitating efficient multiplication, the medium should be supplemented with glucose (3%) as a carbon source and meta topolin (1 mg/L) as cytokinin. Two-bud explant should be used for subculture as it promoted superior shoot proliferation. Concurrent ex vitro rooting cum hardening was possible even without auxin treatment. The present protocol could be useful for large-scale production of quality planting material of this underexploited tropical species.
format	article
author	A A Waman P Bohra R Karthika Devi J Pixy
author_facet	A A Waman P Bohra R Karthika Devi J Pixy
author_sort	A A Waman
title	In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size
title_short	In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size
title_full	In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size
title_fullStr	In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size
title_full_unstemmed	In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size
title_sort	in vitro multiplication protocol for curcuma mangga : studies on carbon, cytokinin source and explant size
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2021
url	https://doaj.org/article/37e45cbc1d3641d58d83a3cce119d725
work_keys_str_mv	AT aawaman invitromultiplicationprotocolforcurcumamanggastudiesoncarboncytokininsourceandexplantsize AT pbohra invitromultiplicationprotocolforcurcumamanggastudiesoncarboncytokininsourceandexplantsize AT rkarthikadevi invitromultiplicationprotocolforcurcumamanggastudiesoncarboncytokininsourceandexplantsize AT jpixy invitromultiplicationprotocolforcurcumamanggastudiesoncarboncytokininsourceandexplantsize
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In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size

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