Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment

Danbo Yang1, Sang Van2, Jian Liu2, Jing Wang1, Xinguo Jiang3, Yiting Wang1, Lei Yu1,2 1Biomedical Engineering and Technology Institute, Institutes for Advanced Interdisciplinary Research, East China Normal University, Shanghai, China; 2Biomedical Group, Nitto Denko Technical Corporation, Oceanside,...

Full description

Saved in:

Bibliographic Details
Main Authors:	Yang D, Van S, Liu J, Wang J, Jiang X, Wang Y, Yu L
Format:	article
Language:	EN
Published:	Dove Medical Press 2011
Subjects:	Medicine (General) R5-920
Online Access:	https://doaj.org/article/37e794d6ff434b23b179b7cb2e894a20
Tags:	Add Tag No Tags, Be the first to tag this record!

id	oai:doaj.org-article:37e794d6ff434b23b179b7cb2e894a20
record_format	dspace
spelling	oai:doaj.org-article:37e794d6ff434b23b179b7cb2e894a202021-12-02T05:32:33ZPhysicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment1176-91141178-2013https://doaj.org/article/37e794d6ff434b23b179b7cb2e894a202011-10-01T00:00:00Zhttp://www.dovepress.com/physicochemical-properties-and-biocompatibility-of-a-polymer-paclitaxe-a8530https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Danbo Yang1, Sang Van2, Jian Liu2, Jing Wang1, Xinguo Jiang3, Yiting Wang1, Lei Yu1,2 1Biomedical Engineering and Technology Institute, Institutes for Advanced Interdisciplinary Research, East China Normal University, Shanghai, China; 2Biomedical Group, Nitto Denko Technical Corporation, Oceanside, CA; 3School of Pharmacy, Fudan University, Shanghai, ChinaBackground: Poly(L-&gamma;-glutamylglutamine) paclitaxel (PGG-PTX) conjugate is a non-diblock polymeric drug nanoparticle intended to improve the therapeutic index of paclitaxel. The purpose of the present study was to elucidate further the physicochemical properties of PGG-PTX in order to proceed with its clinical development.Methods and results: PGG-PTX was designed by integration of a hydrophobic paclitaxel conjugate through an added hydrophilic glutamic acid onto poly(L-glutamic acid). The addition of a flexible glutamic linker between PGA and paclitaxel resulted in spontaneous self-assembly of a PGG-PTX conjugate into nanoparticles. The PGG-PTX conjugate was stable as a lyophilized solid form. An in vitro viability experiment showed that PGG-PTX was effective after a longer incubation period, the same trend as Taxol. In vitro studies using NCI-H460 and B16F0 cancer cells demonstrated significantly high cellular uptake after 30 minutes of incubation. The in vivo biocompatibility of PGG-PTX conjugate was evaluated in the NCI-H460 tumor model, the assessment of tissue seemed to be normal after 21 days of treatment.Conclusion: These results are encouraging for further development of non-block polymeric paclitaxel nanoparticles for treatment of cancer.Keywords: polymer conjugate, paclitaxel, poly(L-&gamma;-glutamylglutamine), drug delivery, physicochemical properties, nanoparticlesYang DVan SLiu JWang JJiang XWang YYu LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2557-2566 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yang D Van S Liu J Wang J Jiang X Wang Y Yu L Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
description	Danbo Yang1, Sang Van2, Jian Liu2, Jing Wang1, Xinguo Jiang3, Yiting Wang1, Lei Yu1,2 1Biomedical Engineering and Technology Institute, Institutes for Advanced Interdisciplinary Research, East China Normal University, Shanghai, China; 2Biomedical Group, Nitto Denko Technical Corporation, Oceanside, CA; 3School of Pharmacy, Fudan University, Shanghai, ChinaBackground: Poly(L-&gamma;-glutamylglutamine) paclitaxel (PGG-PTX) conjugate is a non-diblock polymeric drug nanoparticle intended to improve the therapeutic index of paclitaxel. The purpose of the present study was to elucidate further the physicochemical properties of PGG-PTX in order to proceed with its clinical development.Methods and results: PGG-PTX was designed by integration of a hydrophobic paclitaxel conjugate through an added hydrophilic glutamic acid onto poly(L-glutamic acid). The addition of a flexible glutamic linker between PGA and paclitaxel resulted in spontaneous self-assembly of a PGG-PTX conjugate into nanoparticles. The PGG-PTX conjugate was stable as a lyophilized solid form. An in vitro viability experiment showed that PGG-PTX was effective after a longer incubation period, the same trend as Taxol. In vitro studies using NCI-H460 and B16F0 cancer cells demonstrated significantly high cellular uptake after 30 minutes of incubation. The in vivo biocompatibility of PGG-PTX conjugate was evaluated in the NCI-H460 tumor model, the assessment of tissue seemed to be normal after 21 days of treatment.Conclusion: These results are encouraging for further development of non-block polymeric paclitaxel nanoparticles for treatment of cancer.Keywords: polymer conjugate, paclitaxel, poly(L-&gamma;-glutamylglutamine), drug delivery, physicochemical properties, nanoparticles
format	article
author	Yang D Van S Liu J Wang J Jiang X Wang Y Yu L
author_facet	Yang D Van S Liu J Wang J Jiang X Wang Y Yu L
author_sort	Yang D
title	Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
title_short	Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
title_full	Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
title_fullStr	Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
title_full_unstemmed	Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
title_sort	physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/37e794d6ff434b23b179b7cb2e894a20
work_keys_str_mv	AT yangd physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment AT vans physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment AT liuj physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment AT wangj physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment AT jiangx physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment AT wangy physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment AT yul physicochemicalpropertiesandbiocompatibilityofapolymerpaclitaxelconjugateforcancertreatment
_version_	1718400346054721536

Physicochemical properties and biocompatibility of a polymer-paclitaxel conjugate for cancer treatment

Similar Items