Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose

Abstract While numerous techniques can be used to measure and analyze insulin secretion in isolated islets in culture, assessments of insulin secretion in vivo are typically indirect and only semiquantitative. The CpepSfGFP reporter mouse line allows the in vivo imaging of insulin secretion from ind...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Henriette Frikke-Schmidt, Peter Arvan, Randy J. Seeley, Corentin Cras-Méneur
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37ea758f07794217acf1ad04f2a75fb6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:37ea758f07794217acf1ad04f2a75fb6
record_format	dspace
spelling	oai:doaj.org-article:37ea758f07794217acf1ad04f2a75fb62021-12-02T14:12:46ZImproved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose10.1038/s41598-020-79727-82045-2322https://doaj.org/article/37ea758f07794217acf1ad04f2a75fb62021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79727-8https://doaj.org/toc/2045-2322Abstract While numerous techniques can be used to measure and analyze insulin secretion in isolated islets in culture, assessments of insulin secretion in vivo are typically indirect and only semiquantitative. The CpepSfGFP reporter mouse line allows the in vivo imaging of insulin secretion from individual islets after a glucose stimulation, in live, anesthetized mice. Imaging the whole pancreas at high resolution in live mice to track the response of each individual islet over time includes numerous technical challenges and previous reports were only limited in scope and non-quantitative. Elaborating on this previous model—through the development of an improved methodology addressing anesthesia, temperature control and motion blur—we were able to track and quantify longitudinally insulin content throughout a glucose challenge in up to two hundred individual islets simultaneously. Through this approach we demonstrate quantitatively for the first time that while isolated islets respond homogeneously to glucose in culture, their profiles differ significantly in vivo. Independent of size or location, some islets respond sharply to a glucose stimulation while others barely secrete at all. This platform therefore provides a powerful approach to study the impact of disease, diet, surgery or pharmacological treatments on insulin secretion in the intact pancreas in vivo.Henriette Frikke-SchmidtPeter ArvanRandy J. SeeleyCorentin Cras-MéneurNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Henriette Frikke-Schmidt Peter Arvan Randy J. Seeley Corentin Cras-Méneur Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
description	Abstract While numerous techniques can be used to measure and analyze insulin secretion in isolated islets in culture, assessments of insulin secretion in vivo are typically indirect and only semiquantitative. The CpepSfGFP reporter mouse line allows the in vivo imaging of insulin secretion from individual islets after a glucose stimulation, in live, anesthetized mice. Imaging the whole pancreas at high resolution in live mice to track the response of each individual islet over time includes numerous technical challenges and previous reports were only limited in scope and non-quantitative. Elaborating on this previous model—through the development of an improved methodology addressing anesthesia, temperature control and motion blur—we were able to track and quantify longitudinally insulin content throughout a glucose challenge in up to two hundred individual islets simultaneously. Through this approach we demonstrate quantitatively for the first time that while isolated islets respond homogeneously to glucose in culture, their profiles differ significantly in vivo. Independent of size or location, some islets respond sharply to a glucose stimulation while others barely secrete at all. This platform therefore provides a powerful approach to study the impact of disease, diet, surgery or pharmacological treatments on insulin secretion in the intact pancreas in vivo.
format	article
author	Henriette Frikke-Schmidt Peter Arvan Randy J. Seeley Corentin Cras-Méneur
author_facet	Henriette Frikke-Schmidt Peter Arvan Randy J. Seeley Corentin Cras-Méneur
author_sort	Henriette Frikke-Schmidt
title	Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
title_short	Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
title_full	Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
title_fullStr	Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
title_full_unstemmed	Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
title_sort	improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37ea758f07794217acf1ad04f2a75fb6
work_keys_str_mv	AT henriettefrikkeschmidt improvedinvivoimagingmethodforindividualisletsacrossthemousepancreasrevealsaheterogeneousinsulinsecretionresponsetoglucose AT peterarvan improvedinvivoimagingmethodforindividualisletsacrossthemousepancreasrevealsaheterogeneousinsulinsecretionresponsetoglucose AT randyjseeley improvedinvivoimagingmethodforindividualisletsacrossthemousepancreasrevealsaheterogeneousinsulinsecretionresponsetoglucose AT corentincrasmeneur improvedinvivoimagingmethodforindividualisletsacrossthemousepancreasrevealsaheterogeneousinsulinsecretionresponsetoglucose
_version_	1718391778637250560

Improved in vivo imaging method for individual islets across the mouse pancreas reveals a heterogeneous insulin secretion response to glucose

Ejemplares similares