Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation

The effects of transfection of N-terminal fragment of chromogranin A Vasostatin-1 (VS-1) nanocarriers on formation of abdominal aortic aneurysm (AAA) were discussed, and its mechanism was analyzed. Nanoparticles containing VS-1 genes were prepared by emulsion solvent evaporation method, and property...

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Autores principales:	Pingshan Wang, Wei Wang, Xingxing Peng, Fugui Ruan, Shiyao Yang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	chromogranin a vasostatin-1 nano-carrier abdominal aortic aneurysm rats Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/37ee611f4c4b4d0abe691ffa1fe1e1c1
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spelling	oai:doaj.org-article:37ee611f4c4b4d0abe691ffa1fe1e1c12021-12-01T14:41:00ZEffect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation2165-59792165-598710.1080/21655979.2021.2005222https://doaj.org/article/37ee611f4c4b4d0abe691ffa1fe1e1c12021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005222https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987The effects of transfection of N-terminal fragment of chromogranin A Vasostatin-1 (VS-1) nanocarriers on formation of abdominal aortic aneurysm (AAA) were discussed, and its mechanism was analyzed. Nanoparticles containing VS-1 genes were prepared by emulsion solvent evaporation method, and property of nanoparticles was examined. A total of 30 male SD rats were divided randomly into sham group (normal saline), AAA group (Type I porcine pancreatic elastase), and VS-1 group (Type I porcine pancreatic elastase+VS-1 suspension liquid). The diameter dilation of rats was measured, abdominal aortic morphology was observed by HE staining, and levels of AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) were examined by immunohistochemistry and Western blot. Correlation between AMPK as well as mTOR and diameter dilation was analyzed by Pearson correlation. VS-1 genes in VS-1 nanoparticles were 4.51% and coating efficiency of genes was 88%. Compared with rats in sham group, diameter dilation of rats in AAA group increased, damage of abdominal aorta in rats was obvious, p-AMPK decreased, and p-mTOR increased in AAA group. Compared with AAA group, diameter dilation of rats in VS-1 group decreased, abdominal aorta of rats was improved, p-AMPK increased, and p-mTOR decreased. The comparison of all above indicators had statistical meaning (P < 0.05). p-AMPK and p-mTOR were negatively (r = −0.9150 and P = 0.006) and positively correlated with the diameter dilation (r = −0.9206 and P = 0.001). VS-1 nanoparticles could inhibit the formation of AAA, which might be related to the activation of AMPK/mTOR signal path.Pingshan WangWei WangXingxing PengFugui RuanShiyao YangTaylor & Francis Grouparticlechromogranin avasostatin-1nano-carrierabdominal aortic aneurysmratsBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 11018-11029 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chromogranin a vasostatin-1 nano-carrier abdominal aortic aneurysm rats Biotechnology TP248.13-248.65
spellingShingle	chromogranin a vasostatin-1 nano-carrier abdominal aortic aneurysm rats Biotechnology TP248.13-248.65 Pingshan Wang Wei Wang Xingxing Peng Fugui Ruan Shiyao Yang Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
description	The effects of transfection of N-terminal fragment of chromogranin A Vasostatin-1 (VS-1) nanocarriers on formation of abdominal aortic aneurysm (AAA) were discussed, and its mechanism was analyzed. Nanoparticles containing VS-1 genes were prepared by emulsion solvent evaporation method, and property of nanoparticles was examined. A total of 30 male SD rats were divided randomly into sham group (normal saline), AAA group (Type I porcine pancreatic elastase), and VS-1 group (Type I porcine pancreatic elastase+VS-1 suspension liquid). The diameter dilation of rats was measured, abdominal aortic morphology was observed by HE staining, and levels of AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) were examined by immunohistochemistry and Western blot. Correlation between AMPK as well as mTOR and diameter dilation was analyzed by Pearson correlation. VS-1 genes in VS-1 nanoparticles were 4.51% and coating efficiency of genes was 88%. Compared with rats in sham group, diameter dilation of rats in AAA group increased, damage of abdominal aorta in rats was obvious, p-AMPK decreased, and p-mTOR increased in AAA group. Compared with AAA group, diameter dilation of rats in VS-1 group decreased, abdominal aorta of rats was improved, p-AMPK increased, and p-mTOR decreased. The comparison of all above indicators had statistical meaning (P < 0.05). p-AMPK and p-mTOR were negatively (r = −0.9150 and P = 0.006) and positively correlated with the diameter dilation (r = −0.9206 and P = 0.001). VS-1 nanoparticles could inhibit the formation of AAA, which might be related to the activation of AMPK/mTOR signal path.
format	article
author	Pingshan Wang Wei Wang Xingxing Peng Fugui Ruan Shiyao Yang
author_facet	Pingshan Wang Wei Wang Xingxing Peng Fugui Ruan Shiyao Yang
author_sort	Pingshan Wang
title	Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
title_short	Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
title_full	Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
title_fullStr	Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
title_full_unstemmed	Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
title_sort	effect of chromogranin a n-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/37ee611f4c4b4d0abe691ffa1fe1e1c1
work_keys_str_mv	AT pingshanwang effectofchromograninanterminalfragmentvasostatin1nanocarriertransfectiononabdominalaorticaneurysmformation AT weiwang effectofchromograninanterminalfragmentvasostatin1nanocarriertransfectiononabdominalaorticaneurysmformation AT xingxingpeng effectofchromograninanterminalfragmentvasostatin1nanocarriertransfectiononabdominalaorticaneurysmformation AT fuguiruan effectofchromograninanterminalfragmentvasostatin1nanocarriertransfectiononabdominalaorticaneurysmformation AT shiyaoyang effectofchromograninanterminalfragmentvasostatin1nanocarriertransfectiononabdominalaorticaneurysmformation
_version_	1718404985008422912

Effect of chromogranin A N-terminal fragment vasostatin-1 nano-carrier transfection on abdominal aortic aneurysm formation

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