Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis

Stigmasterol (STM), one of the main active components of Achyranthes bidentata, has been shown to effectively inhibit proinflammatory factors and matrix degradation in chondrocytes. However, the effect of STM on interleukin (IL)-1β-induced chondrocytes and its specific mechanism remain unclear. The...

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Autores principales:	Zhisheng Mo, Peiqing Xu, Huanyu Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	stigmasterol knee osteoarthritis chondrocytes ferroptosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/37eee883dbd447f7a982a7936d570bfb
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spelling	oai:doaj.org-article:37eee883dbd447f7a982a7936d570bfb2021-11-26T11:19:49ZStigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis2165-59792165-598710.1080/21655979.2021.2000742https://doaj.org/article/37eee883dbd447f7a982a7936d570bfb2021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2000742https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Stigmasterol (STM), one of the main active components of Achyranthes bidentata, has been shown to effectively inhibit proinflammatory factors and matrix degradation in chondrocytes. However, the effect of STM on interleukin (IL)-1β-induced chondrocytes and its specific mechanism remain unclear. The purpose of the present study was to explore the effect and mechanism of sterol regulatory element binding transcription factor 2 (SREBF2) on IL-1β induced chondrocytes in the presence of STM. CCK-8 was used to detect the effect of STM on the cell viability of mouse chondrogenic cells (ATDC5). After ATDC5 cells were induced by IL-1β, the expression of SREBF2 in osteoarthritis cells was detected by RT-qPCR. The content of iron ion in the cells was detected by using an iron colorimetric assay kit. After further transfection of a SREBF2 overexpressing vector (Oe-SREBF2) or addition of a ferroptosis inhibitor, the expression levels of inflammation and matrix degradation-related proteins were detected via Western blotting. The levels of oxidative stress in cells were determined by using an ELISA kit. The results revealed that STM had no significant effect on the viability of ATDC5 cells. STM reduced IL-1β-induced ATDC5 cell damage and ferroptosis through SREBF2 and enhanced the inhibitory effect of ferroptosis inhibitors on IL-1β-induced ATDC5 cell injury. The present data suggest that STM attenuated chondrocyte injury induced by IL-1β by regulating ferroptosis via down-regulation of SREBF2, and may have potential as a novel therapeutic method for knee osteoarthritis.Zhisheng MoPeiqing XuHuanyu LiTaylor & Francis Grouparticlestigmasterolknee osteoarthritischondrocytesferroptosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 9332-9340 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	stigmasterol knee osteoarthritis chondrocytes ferroptosis Biotechnology TP248.13-248.65
spellingShingle	stigmasterol knee osteoarthritis chondrocytes ferroptosis Biotechnology TP248.13-248.65 Zhisheng Mo Peiqing Xu Huanyu Li Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
description	Stigmasterol (STM), one of the main active components of Achyranthes bidentata, has been shown to effectively inhibit proinflammatory factors and matrix degradation in chondrocytes. However, the effect of STM on interleukin (IL)-1β-induced chondrocytes and its specific mechanism remain unclear. The purpose of the present study was to explore the effect and mechanism of sterol regulatory element binding transcription factor 2 (SREBF2) on IL-1β induced chondrocytes in the presence of STM. CCK-8 was used to detect the effect of STM on the cell viability of mouse chondrogenic cells (ATDC5). After ATDC5 cells were induced by IL-1β, the expression of SREBF2 in osteoarthritis cells was detected by RT-qPCR. The content of iron ion in the cells was detected by using an iron colorimetric assay kit. After further transfection of a SREBF2 overexpressing vector (Oe-SREBF2) or addition of a ferroptosis inhibitor, the expression levels of inflammation and matrix degradation-related proteins were detected via Western blotting. The levels of oxidative stress in cells were determined by using an ELISA kit. The results revealed that STM had no significant effect on the viability of ATDC5 cells. STM reduced IL-1β-induced ATDC5 cell damage and ferroptosis through SREBF2 and enhanced the inhibitory effect of ferroptosis inhibitors on IL-1β-induced ATDC5 cell injury. The present data suggest that STM attenuated chondrocyte injury induced by IL-1β by regulating ferroptosis via down-regulation of SREBF2, and may have potential as a novel therapeutic method for knee osteoarthritis.
format	article
author	Zhisheng Mo Peiqing Xu Huanyu Li
author_facet	Zhisheng Mo Peiqing Xu Huanyu Li
author_sort	Zhisheng Mo
title	Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
title_short	Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
title_full	Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
title_fullStr	Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
title_full_unstemmed	Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
title_sort	stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/37eee883dbd447f7a982a7936d570bfb
work_keys_str_mv	AT zhishengmo stigmasterolalleviatesinterleukin1betainducedchondrocyteinjurybydownregulatingsterolregulatoryelementbindingtranscriptionfactor2toregulateferroptosis AT peiqingxu stigmasterolalleviatesinterleukin1betainducedchondrocyteinjurybydownregulatingsterolregulatoryelementbindingtranscriptionfactor2toregulateferroptosis AT huanyuli stigmasterolalleviatesinterleukin1betainducedchondrocyteinjurybydownregulatingsterolregulatoryelementbindingtranscriptionfactor2toregulateferroptosis
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Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis

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