Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings

Fernando F Rios-Pimentel,1 Run Chang,2 Thomas J Webster,2 Magdalena M Méndez-González,3 Miguel García-Rocha4 1Nanociencias y Nanotecnología, CINVESTAV-IPN, Ciudad de Mexico, México; 2Department of Chemical Engineering, Northeastern University,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rios-Pimentel FF, Chang R, Webster TJ, Méndez-González MM, García-Rocha M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Hydroxyapatite APNPs coatings precipitation XRD FTIR TEM orthopedic applications. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/37f50768a3204c3c8ca95d7d6fbe44c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:37f50768a3204c3c8ca95d7d6fbe44c9
record_format	dspace
spelling	oai:doaj.org-article:37f50768a3204c3c8ca95d7d6fbe44c92021-12-02T06:09:01ZGreater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings1178-2013https://doaj.org/article/37f50768a3204c3c8ca95d7d6fbe44c92019-05-01T00:00:00Zhttps://www.dovepress.com/greater-osteoblast-densities-due-to-the-addition-of-amphiphilic-peptid-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fernando F Rios-Pimentel,1 Run Chang,2 Thomas J Webster,2 Magdalena M Méndez-González,3 Miguel García-Rocha4 1Nanociencias y Nanotecnología, CINVESTAV-IPN, Ciudad de Mexico, México; 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 3Departamento de Física, ESFM-IPN, Ciudad de Mexico, México; 4Departamento de Física, CINVESTAV-IPN, Ciudad de Mexico, México Background: In vitro and in vivo studies have shown that metallic implants coated with nano hydroxyapatite (HA) reduce the time needed for complete osseointegration compared to metallic implants coated with conventional or micron-sized HA. Moreover, due to their biologically inspired nanometer dimensions, amphiphilic peptide nanoparticles (APNPs) can also promote osteoblast attachment and enhance other cell functions if used as a coating material. Coatings made of HA and APNPs could improve osteoblast functions, but have never been tested. Purpose: The objective of this study was to prepare coatings of nanocrystalline HA and APNPs on poly(2-hydroxyethyl methacrylate) (pHEMA) coatings in order to improve osteoblast (bone-forming cells) adhesion and cell density.Methods: HA was synthesized by a wet chemical process. Coatings were synthesized with different conditions and components.Results: X-ray diffraction infrared spectroscopy, transmission electron microscopy, and electron diffraction showed that nanocrystalline HA was synthesized with an expected nano size and shape distribution but with low impurities. pHEMA hydrogels with HA and APNPs increased osteoblast densities after 3 days compared to controls.Conclusion: Since cell proliferation is a prerequisite function for bone formation, these results imply that the current materials should be tested for a wide range of orthopedic applications. Keywords: orthopedic applications, XRD, FTIR, TEM, SEM, cell studiesRios-Pimentel FFChang RWebster TJMéndez-González MMGarcía-Rocha MDove Medical PressarticleHydroxyapatiteAPNPscoatingsprecipitationXRDFTIRTEMorthopedic applications.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3265-3272 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hydroxyapatite APNPs coatings precipitation XRD FTIR TEM orthopedic applications. Medicine (General) R5-920
spellingShingle	Hydroxyapatite APNPs coatings precipitation XRD FTIR TEM orthopedic applications. Medicine (General) R5-920 Rios-Pimentel FF Chang R Webster TJ Méndez-González MM García-Rocha M Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
description	Fernando F Rios-Pimentel,1 Run Chang,2 Thomas J Webster,2 Magdalena M Méndez-González,3 Miguel García-Rocha4 1Nanociencias y Nanotecnología, CINVESTAV-IPN, Ciudad de Mexico, México; 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 3Departamento de Física, ESFM-IPN, Ciudad de Mexico, México; 4Departamento de Física, CINVESTAV-IPN, Ciudad de Mexico, México Background: In vitro and in vivo studies have shown that metallic implants coated with nano hydroxyapatite (HA) reduce the time needed for complete osseointegration compared to metallic implants coated with conventional or micron-sized HA. Moreover, due to their biologically inspired nanometer dimensions, amphiphilic peptide nanoparticles (APNPs) can also promote osteoblast attachment and enhance other cell functions if used as a coating material. Coatings made of HA and APNPs could improve osteoblast functions, but have never been tested. Purpose: The objective of this study was to prepare coatings of nanocrystalline HA and APNPs on poly(2-hydroxyethyl methacrylate) (pHEMA) coatings in order to improve osteoblast (bone-forming cells) adhesion and cell density.Methods: HA was synthesized by a wet chemical process. Coatings were synthesized with different conditions and components.Results: X-ray diffraction infrared spectroscopy, transmission electron microscopy, and electron diffraction showed that nanocrystalline HA was synthesized with an expected nano size and shape distribution but with low impurities. pHEMA hydrogels with HA and APNPs increased osteoblast densities after 3 days compared to controls.Conclusion: Since cell proliferation is a prerequisite function for bone formation, these results imply that the current materials should be tested for a wide range of orthopedic applications. Keywords: orthopedic applications, XRD, FTIR, TEM, SEM, cell studies
format	article
author	Rios-Pimentel FF Chang R Webster TJ Méndez-González MM García-Rocha M
author_facet	Rios-Pimentel FF Chang R Webster TJ Méndez-González MM García-Rocha M
author_sort	Rios-Pimentel FF
title	Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
title_short	Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
title_full	Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
title_fullStr	Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
title_full_unstemmed	Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
title_sort	greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/37f50768a3204c3c8ca95d7d6fbe44c9
work_keys_str_mv	AT riospimentelff greaterosteoblastdensitiesduetotheadditionofamphiphilicpeptidenanoparticlestonanohydroxyapatitecoatings AT changr greaterosteoblastdensitiesduetotheadditionofamphiphilicpeptidenanoparticlestonanohydroxyapatitecoatings AT webstertj greaterosteoblastdensitiesduetotheadditionofamphiphilicpeptidenanoparticlestonanohydroxyapatitecoatings AT mendezgonzalezmm greaterosteoblastdensitiesduetotheadditionofamphiphilicpeptidenanoparticlestonanohydroxyapatitecoatings AT garciarocham greaterosteoblastdensitiesduetotheadditionofamphiphilicpeptidenanoparticlestonanohydroxyapatitecoatings
_version_	1718400046094876672

Greater osteoblast densities due to the addition of amphiphilic peptide nanoparticles to nano hydroxyapatite coatings

Ejemplares similares