Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.

<h4>Background</h4>The light-emitting Vibrios provide excellent material for studying the interaction of cellular communication with growth rate because bioluminescence is a convenient marker for quorum sensing. However, the use of bioluminescence as a marker is complicated because biolu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zeena E Nackerdien, Alexander Keynan, Bonnie L Bassler, Joshua Lederberg, David S Thaler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/37f5e7af58314b188a85f364c6809ff0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:37f5e7af58314b188a85f364c6809ff0
record_format	dspace
spelling	oai:doaj.org-article:37f5e7af58314b188a85f364c6809ff02021-11-25T06:13:16ZQuorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.1932-620310.1371/journal.pone.0001671https://doaj.org/article/37f5e7af58314b188a85f364c6809ff02008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18301749/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The light-emitting Vibrios provide excellent material for studying the interaction of cellular communication with growth rate because bioluminescence is a convenient marker for quorum sensing. However, the use of bioluminescence as a marker is complicated because bioluminescence itself may affect growth rate, e.g. by diverting energy.<h4>Methodology/principal findings</h4>The marker effect was explored via growth rate studies in isogenic Vibrio harveyi (Vh) strains altered in quorum sensing on the one hand, and bioluminescence on the other. By hypothesis, growth rate is energy limited: mutants deficient in quorum sensing grow faster because wild type quorum sensing unleashes bioluminescence and bioluminescence diverts energy. Findings reported here confirm a role for bioluminescence in limiting Vh growth rate, at least under the conditions tested. However, the results argue that the bioluminescence is insufficient to explain the relationship of growth rate and quorum sensing in Vh. A Vh mutant null for all genes encoding the bioluminescence pathway grew faster than wild type but not as fast as null mutants in quorum sensing. Vh quorum sensing mutants showed altered growth rates that do not always rank with their relative increase or decrease in bioluminescence. In addition, the cell-free culture fluids of a rapidly growing Vibrio parahaemolyticus (Vp) strain increased the growth rate of wild type Vh without significantly altering Vh's bioluminescence. The same cell-free culture fluid increased the bioluminescence of Vh quorum mutants.<h4>Conclusions/significance</h4>The effect of quorum sensing on Vh growth rate can be either positive or negative and includes both bioluminescence-dependent and independent components. Bioluminescence tends to slow growth rate but not enough to account for the effects of quorum sensing on growth rate.Zeena E NackerdienAlexander KeynanBonnie L BasslerJoshua LederbergDavid S ThalerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 2, p e1671 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zeena E Nackerdien Alexander Keynan Bonnie L Bassler Joshua Lederberg David S Thaler Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
description	<h4>Background</h4>The light-emitting Vibrios provide excellent material for studying the interaction of cellular communication with growth rate because bioluminescence is a convenient marker for quorum sensing. However, the use of bioluminescence as a marker is complicated because bioluminescence itself may affect growth rate, e.g. by diverting energy.<h4>Methodology/principal findings</h4>The marker effect was explored via growth rate studies in isogenic Vibrio harveyi (Vh) strains altered in quorum sensing on the one hand, and bioluminescence on the other. By hypothesis, growth rate is energy limited: mutants deficient in quorum sensing grow faster because wild type quorum sensing unleashes bioluminescence and bioluminescence diverts energy. Findings reported here confirm a role for bioluminescence in limiting Vh growth rate, at least under the conditions tested. However, the results argue that the bioluminescence is insufficient to explain the relationship of growth rate and quorum sensing in Vh. A Vh mutant null for all genes encoding the bioluminescence pathway grew faster than wild type but not as fast as null mutants in quorum sensing. Vh quorum sensing mutants showed altered growth rates that do not always rank with their relative increase or decrease in bioluminescence. In addition, the cell-free culture fluids of a rapidly growing Vibrio parahaemolyticus (Vp) strain increased the growth rate of wild type Vh without significantly altering Vh's bioluminescence. The same cell-free culture fluid increased the bioluminescence of Vh quorum mutants.<h4>Conclusions/significance</h4>The effect of quorum sensing on Vh growth rate can be either positive or negative and includes both bioluminescence-dependent and independent components. Bioluminescence tends to slow growth rate but not enough to account for the effects of quorum sensing on growth rate.
format	article
author	Zeena E Nackerdien Alexander Keynan Bonnie L Bassler Joshua Lederberg David S Thaler
author_facet	Zeena E Nackerdien Alexander Keynan Bonnie L Bassler Joshua Lederberg David S Thaler
author_sort	Zeena E Nackerdien
title	Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
title_short	Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
title_full	Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
title_fullStr	Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
title_full_unstemmed	Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
title_sort	quorum sensing influences vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/37f5e7af58314b188a85f364c6809ff0
work_keys_str_mv	AT zeenaenackerdien quorumsensinginfluencesvibrioharveyigrowthratesinamannernotfullyaccountedforbythemarkereffectofbioluminescence AT alexanderkeynan quorumsensinginfluencesvibrioharveyigrowthratesinamannernotfullyaccountedforbythemarkereffectofbioluminescence AT bonnielbassler quorumsensinginfluencesvibrioharveyigrowthratesinamannernotfullyaccountedforbythemarkereffectofbioluminescence AT joshualederberg quorumsensinginfluencesvibrioharveyigrowthratesinamannernotfullyaccountedforbythemarkereffectofbioluminescence AT davidsthaler quorumsensinginfluencesvibrioharveyigrowthratesinamannernotfullyaccountedforbythemarkereffectofbioluminescence
_version_	1718414035004686336

Quorum sensing influences Vibrio harveyi growth rates in a manner not fully accounted for by the marker effect of bioluminescence.

Ejemplares similares