Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Direct observation of G-quadruplexes (G4s) in live cells is challenging. Here the authors report a method to identify G4s within the nuclei of live and fixed cells using a fluorescent probe combined with fluorescence lifetime imaging microscopy.

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Autores principales:	Peter A. Summers, Benjamin W. Lewis, Jorge Gonzalez-Garcia, Rosa M. Porreca, Aaron H. M. Lim, Paolo Cadinu, Nerea Martin-Pintado, David J. Mann, Joshua B. Edel, Jean Baptiste Vannier, Marina K. Kuimova, Ramon Vilar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/37fc31773c234ea49cb172f66805dd72
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spelling	oai:doaj.org-article:37fc31773c234ea49cb172f66805dd722021-12-02T15:08:00ZVisualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy10.1038/s41467-020-20414-72041-1723https://doaj.org/article/37fc31773c234ea49cb172f66805dd722021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20414-7https://doaj.org/toc/2041-1723Direct observation of G-quadruplexes (G4s) in live cells is challenging. Here the authors report a method to identify G4s within the nuclei of live and fixed cells using a fluorescent probe combined with fluorescence lifetime imaging microscopy.Peter A. SummersBenjamin W. LewisJorge Gonzalez-GarciaRosa M. PorrecaAaron H. M. LimPaolo CadinuNerea Martin-PintadoDavid J. MannJoshua B. EdelJean Baptiste VannierMarina K. KuimovaRamon VilarNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Peter A. Summers Benjamin W. Lewis Jorge Gonzalez-Garcia Rosa M. Porreca Aaron H. M. Lim Paolo Cadinu Nerea Martin-Pintado David J. Mann Joshua B. Edel Jean Baptiste Vannier Marina K. Kuimova Ramon Vilar Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
description	Direct observation of G-quadruplexes (G4s) in live cells is challenging. Here the authors report a method to identify G4s within the nuclei of live and fixed cells using a fluorescent probe combined with fluorescence lifetime imaging microscopy.
format	article
author	Peter A. Summers Benjamin W. Lewis Jorge Gonzalez-Garcia Rosa M. Porreca Aaron H. M. Lim Paolo Cadinu Nerea Martin-Pintado David J. Mann Joshua B. Edel Jean Baptiste Vannier Marina K. Kuimova Ramon Vilar
author_facet	Peter A. Summers Benjamin W. Lewis Jorge Gonzalez-Garcia Rosa M. Porreca Aaron H. M. Lim Paolo Cadinu Nerea Martin-Pintado David J. Mann Joshua B. Edel Jean Baptiste Vannier Marina K. Kuimova Ramon Vilar
author_sort	Peter A. Summers
title	Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
title_short	Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
title_full	Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
title_fullStr	Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
title_full_unstemmed	Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy
title_sort	visualising g-quadruplex dna dynamics in live cells by fluorescence lifetime imaging microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/37fc31773c234ea49cb172f66805dd72
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Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

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