Mucus organisation is shaped by colonic content; a new view

Abstract The colonic mucus barrier is commonly described as a continuous double layer covering the epithelium, separating the microbiota from the intestinal tissue. This model is currently considered valid throughout the colon. The colon is characterised by regional anatomo-functional specificities...

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Autores principales:	J. B. J. Kamphuis, M. Mercier-Bonin, H. Eutamène, V. Theodorou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3801f54efd15448188a7ef6c0743d2f6
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spelling	oai:doaj.org-article:3801f54efd15448188a7ef6c0743d2f62021-12-02T11:40:59ZMucus organisation is shaped by colonic content; a new view10.1038/s41598-017-08938-32045-2322https://doaj.org/article/3801f54efd15448188a7ef6c0743d2f62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08938-3https://doaj.org/toc/2045-2322Abstract The colonic mucus barrier is commonly described as a continuous double layer covering the epithelium, separating the microbiota from the intestinal tissue. This model is currently considered valid throughout the colon. The colon is characterised by regional anatomo-functional specificities such as presence and consistency of contents and location. In this study, we characterised the organisation of the colonic mucus barrier in proximal and distal colon of rodents by histological and FISH staining, taking into account aforementioned specificities. By using longitudinal sections and imaging extensive areas of tissue with and without colonic contents, we have obtained a spatiotemporal overview of mucus organisation in the colon. We describe for the first time that the colonic mucus layer covers the faeces instead of the epithelium in the distal colon. This faecal mucus layer confines the microbiota to the faeces and prevents it from remaining in empty distal colon. In the proximal colon, the mucus did not form a separating layer between bacteria and epithelium. We conclude that the organisation of colonic mucus is reliant on the presence of the colonic content, and the location within the colon. Our findings reopen the discussion on the nature of the colonic mucus barrier.J. B. J. KamphuisM. Mercier-BoninH. EutamèneV. TheodorouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. B. J. Kamphuis M. Mercier-Bonin H. Eutamène V. Theodorou Mucus organisation is shaped by colonic content; a new view
description	Abstract The colonic mucus barrier is commonly described as a continuous double layer covering the epithelium, separating the microbiota from the intestinal tissue. This model is currently considered valid throughout the colon. The colon is characterised by regional anatomo-functional specificities such as presence and consistency of contents and location. In this study, we characterised the organisation of the colonic mucus barrier in proximal and distal colon of rodents by histological and FISH staining, taking into account aforementioned specificities. By using longitudinal sections and imaging extensive areas of tissue with and without colonic contents, we have obtained a spatiotemporal overview of mucus organisation in the colon. We describe for the first time that the colonic mucus layer covers the faeces instead of the epithelium in the distal colon. This faecal mucus layer confines the microbiota to the faeces and prevents it from remaining in empty distal colon. In the proximal colon, the mucus did not form a separating layer between bacteria and epithelium. We conclude that the organisation of colonic mucus is reliant on the presence of the colonic content, and the location within the colon. Our findings reopen the discussion on the nature of the colonic mucus barrier.
format	article
author	J. B. J. Kamphuis M. Mercier-Bonin H. Eutamène V. Theodorou
author_facet	J. B. J. Kamphuis M. Mercier-Bonin H. Eutamène V. Theodorou
author_sort	J. B. J. Kamphuis
title	Mucus organisation is shaped by colonic content; a new view
title_short	Mucus organisation is shaped by colonic content; a new view
title_full	Mucus organisation is shaped by colonic content; a new view
title_fullStr	Mucus organisation is shaped by colonic content; a new view
title_full_unstemmed	Mucus organisation is shaped by colonic content; a new view
title_sort	mucus organisation is shaped by colonic content; a new view
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3801f54efd15448188a7ef6c0743d2f6
work_keys_str_mv	AT jbjkamphuis mucusorganisationisshapedbycoloniccontentanewview AT mmercierbonin mucusorganisationisshapedbycoloniccontentanewview AT heutamene mucusorganisationisshapedbycoloniccontentanewview AT vtheodorou mucusorganisationisshapedbycoloniccontentanewview
_version_	1718395454348066816

Mucus organisation is shaped by colonic content; a new view

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