Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics

Abstract The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing...

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Autores principales:	Laura Magro, Béatrice Jacquelin, Camille Escadafal, Pierre Garneret, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafaye, Patrick Tabeling
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3807c101be6d41ce89a7a5809531f806
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spelling	oai:doaj.org-article:3807c101be6d41ce89a7a5809531f8062021-12-02T12:32:50ZPaper-based RNA detection and multiplexed analysis for Ebola virus diagnostics10.1038/s41598-017-00758-92045-2322https://doaj.org/article/3807c101be6d41ce89a7a5809531f8062017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00758-9https://doaj.org/toc/2045-2322Abstract The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing costs, simplifying procedures and enabling multiplexing, paper microfluidics has the potential to considerably facilitate their accessibility. However, most of the studies performed in this area have not quit the lab. This letter brings NAAT on paper closer to the field, by using clinical samples and operating in a resource-limited setting. We first performed isothermal reverse transcription and Recombinase Polymerase Amplification (RT-RPA) of synthetic Ribonucleic Acid (RNA) of Ebola virus using paper microfluidics devices. We further applied this method in Guinea to detect the presence of Ebola virus in human sample RNA extracts, with minimal facilities (carry-on detection device and freeze-dried reagents on paper). RT-RPA results were available in few minutes and demonstrate a sensitivity of 90.0% compared to the gold-standard RT-PCR on a set of 43 patient samples. Furthermore, the realization of a nine-spot multilayered device achieving the parallel detection of three distinct RNA sequences opens a route toward the detection of multiple viral strains or pathogens.Laura MagroBéatrice JacquelinCamille EscadafalPierre GarneretAurélia KwasiborskiJean-Claude ManuguerraFabrice MontiAnavaj SakuntabhaiJessica VanhomwegenPierre LafayePatrick TabelingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Magro Béatrice Jacquelin Camille Escadafal Pierre Garneret Aurélia Kwasiborski Jean-Claude Manuguerra Fabrice Monti Anavaj Sakuntabhai Jessica Vanhomwegen Pierre Lafaye Patrick Tabeling Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics
description	Abstract The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing costs, simplifying procedures and enabling multiplexing, paper microfluidics has the potential to considerably facilitate their accessibility. However, most of the studies performed in this area have not quit the lab. This letter brings NAAT on paper closer to the field, by using clinical samples and operating in a resource-limited setting. We first performed isothermal reverse transcription and Recombinase Polymerase Amplification (RT-RPA) of synthetic Ribonucleic Acid (RNA) of Ebola virus using paper microfluidics devices. We further applied this method in Guinea to detect the presence of Ebola virus in human sample RNA extracts, with minimal facilities (carry-on detection device and freeze-dried reagents on paper). RT-RPA results were available in few minutes and demonstrate a sensitivity of 90.0% compared to the gold-standard RT-PCR on a set of 43 patient samples. Furthermore, the realization of a nine-spot multilayered device achieving the parallel detection of three distinct RNA sequences opens a route toward the detection of multiple viral strains or pathogens.
format	article
author	Laura Magro Béatrice Jacquelin Camille Escadafal Pierre Garneret Aurélia Kwasiborski Jean-Claude Manuguerra Fabrice Monti Anavaj Sakuntabhai Jessica Vanhomwegen Pierre Lafaye Patrick Tabeling
author_facet	Laura Magro Béatrice Jacquelin Camille Escadafal Pierre Garneret Aurélia Kwasiborski Jean-Claude Manuguerra Fabrice Monti Anavaj Sakuntabhai Jessica Vanhomwegen Pierre Lafaye Patrick Tabeling
author_sort	Laura Magro
title	Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics
title_short	Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics
title_full	Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics
title_fullStr	Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics
title_full_unstemmed	Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics
title_sort	paper-based rna detection and multiplexed analysis for ebola virus diagnostics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3807c101be6d41ce89a7a5809531f806
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Paper-based RNA detection and multiplexed analysis for Ebola virus diagnostics

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