Fabrication of fluorescent pH-responsive protein–textile composites

Abstract Wearable pH sensors are useful tools in the healthcare and fitness industries, allowing consumers to access information related to their health in a convenient manner via the monitoring of body fluids. In this work, we tailored novel protein–textile composites to fluorescently respond to ch...

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Autores principales:	Dalia Jane Saldanha, Zahra Abdali, Daniel Modafferi, Bita Janfeshan, Noémie-Manuelle Dorval Courchesne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/381757a03ccb45a788658ffedca6128b
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spelling	oai:doaj.org-article:381757a03ccb45a788658ffedca6128b2021-12-02T16:35:41ZFabrication of fluorescent pH-responsive protein–textile composites10.1038/s41598-020-70079-x2045-2322https://doaj.org/article/381757a03ccb45a788658ffedca6128b2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70079-xhttps://doaj.org/toc/2045-2322Abstract Wearable pH sensors are useful tools in the healthcare and fitness industries, allowing consumers to access information related to their health in a convenient manner via the monitoring of body fluids. In this work, we tailored novel protein–textile composites to fluorescently respond to changing pH. To do so, we used amyloid curli fibers, a key component in the extracellular matrix of Escherichia coli, as genetic scaffold to fuse a pH-responsive fluorescent protein, pHuji. Engineered amyloids form macroscopic and environmentally resistant aggregates that we isolated to use as stand-alone hydrogel-based sensors, and that we trapped within textile matrices to create responsive bio-composites. We showed that these composites were mechanically robust and vapor-permeable, thus exhibiting favorable characteristics for wearable platforms. CsgA–pHuji fibers integrated in the textile allowed the final device to respond to pH changes and distinguish between alkaline and acidic solutions. We demonstrated that the resulting composites could sustain their fluorescence response over days, and that their sensing ability was reversible for at least 10 high/low pH cycles, highlighting their potential for continuous monitoring. Overall, we introduced a biosynthesized amyloid-based textile composite that could be used as biosensing patch for a variety of applications in the smart textile industry.Dalia Jane SaldanhaZahra AbdaliDaniel ModafferiBita JanfeshanNoémie-Manuelle Dorval CourchesneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dalia Jane Saldanha Zahra Abdali Daniel Modafferi Bita Janfeshan Noémie-Manuelle Dorval Courchesne Fabrication of fluorescent pH-responsive protein–textile composites
description	Abstract Wearable pH sensors are useful tools in the healthcare and fitness industries, allowing consumers to access information related to their health in a convenient manner via the monitoring of body fluids. In this work, we tailored novel protein–textile composites to fluorescently respond to changing pH. To do so, we used amyloid curli fibers, a key component in the extracellular matrix of Escherichia coli, as genetic scaffold to fuse a pH-responsive fluorescent protein, pHuji. Engineered amyloids form macroscopic and environmentally resistant aggregates that we isolated to use as stand-alone hydrogel-based sensors, and that we trapped within textile matrices to create responsive bio-composites. We showed that these composites were mechanically robust and vapor-permeable, thus exhibiting favorable characteristics for wearable platforms. CsgA–pHuji fibers integrated in the textile allowed the final device to respond to pH changes and distinguish between alkaline and acidic solutions. We demonstrated that the resulting composites could sustain their fluorescence response over days, and that their sensing ability was reversible for at least 10 high/low pH cycles, highlighting their potential for continuous monitoring. Overall, we introduced a biosynthesized amyloid-based textile composite that could be used as biosensing patch for a variety of applications in the smart textile industry.
format	article
author	Dalia Jane Saldanha Zahra Abdali Daniel Modafferi Bita Janfeshan Noémie-Manuelle Dorval Courchesne
author_facet	Dalia Jane Saldanha Zahra Abdali Daniel Modafferi Bita Janfeshan Noémie-Manuelle Dorval Courchesne
author_sort	Dalia Jane Saldanha
title	Fabrication of fluorescent pH-responsive protein–textile composites
title_short	Fabrication of fluorescent pH-responsive protein–textile composites
title_full	Fabrication of fluorescent pH-responsive protein–textile composites
title_fullStr	Fabrication of fluorescent pH-responsive protein–textile composites
title_full_unstemmed	Fabrication of fluorescent pH-responsive protein–textile composites
title_sort	fabrication of fluorescent ph-responsive protein–textile composites
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/381757a03ccb45a788658ffedca6128b
work_keys_str_mv	AT daliajanesaldanha fabricationoffluorescentphresponsiveproteintextilecomposites AT zahraabdali fabricationoffluorescentphresponsiveproteintextilecomposites AT danielmodafferi fabricationoffluorescentphresponsiveproteintextilecomposites AT bitajanfeshan fabricationoffluorescentphresponsiveproteintextilecomposites AT noemiemanuelledorvalcourchesne fabricationoffluorescentphresponsiveproteintextilecomposites
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Fabrication of fluorescent pH-responsive protein–textile composites

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