Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>

Carbon catabolite repression (CCR) is critical for the preferential utilization of glucose derived from environmental carbon sources and regulated by carbon catabolite repressor A (Cre1/CreA) in filamentous fungi. However, a role of Cre1-mediated CCR in insect-pathogenic fungal utilization of host n...

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Autores principales:	Rehab Abdelmonem Mohamed, Kang Ren, Ya-Ni Mou, Sheng-Hua Ying, Ming-Guang Feng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	entomopathogenic fungi carbon catabolite repression gene expression and regulation host infection virulence biological control Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3821e37ea4be4f0a9a49b895fc6a91a4
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spelling	oai:doaj.org-article:3821e37ea4be4f0a9a49b895fc6a91a42021-11-25T18:05:31ZGenome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>10.3390/jof71108952309-608Xhttps://doaj.org/article/3821e37ea4be4f0a9a49b895fc6a91a42021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/895https://doaj.org/toc/2309-608XCarbon catabolite repression (CCR) is critical for the preferential utilization of glucose derived from environmental carbon sources and regulated by carbon catabolite repressor A (Cre1/CreA) in filamentous fungi. However, a role of Cre1-mediated CCR in insect-pathogenic fungal utilization of host nutrients during normal cuticle infection (NCI) and hemocoel colonization remains explored insufficiently. Here, we report an indispensability of Cre1 for <i>Beauveria</i><i>bassiana</i>’s utilization of nutrients in insect integument and hemocoel. Deletion of <i>cre1</i> resulted in severe defects in radial growth on various media, hypersensitivity to oxidative stress, abolished pathogenicity via NCI or intrahemocoel injection (cuticle-bypassing infection) but no change in conidial hydrophobicity and adherence to insect cuticle. Markedly reduced biomass accumulation in the Δ<i>cre1</i> cultures was directly causative of severe defect in aerial conidiation and reduced secretion of various cuticle-degrading enzymes. The majority (1117) of 1881 dysregulated genes identified from the Δ<i>cre1</i> versus wild-type cultures were significantly downregulated, leading to substantial repression of many enriched function terms and pathways, particularly those involved in carbon and nitrogen metabolisms, cuticle degradation, antioxidant response, cellular transport and homeostasis, and direct/indirect gene mediation. These findings offer a novel insight into profound effect of Cre1 on the insect-pathogenic lifestyle of <i>B. bassiana</i>.Rehab Abdelmonem MohamedKang RenYa-Ni MouSheng-Hua YingMing-Guang FengMDPI AGarticleentomopathogenic fungicarbon catabolite repressiongene expression and regulationhost infectionvirulencebiological controlBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 895, p 895 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	entomopathogenic fungi carbon catabolite repression gene expression and regulation host infection virulence biological control Biology (General) QH301-705.5
spellingShingle	entomopathogenic fungi carbon catabolite repression gene expression and regulation host infection virulence biological control Biology (General) QH301-705.5 Rehab Abdelmonem Mohamed Kang Ren Ya-Ni Mou Sheng-Hua Ying Ming-Guang Feng Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>
description	Carbon catabolite repression (CCR) is critical for the preferential utilization of glucose derived from environmental carbon sources and regulated by carbon catabolite repressor A (Cre1/CreA) in filamentous fungi. However, a role of Cre1-mediated CCR in insect-pathogenic fungal utilization of host nutrients during normal cuticle infection (NCI) and hemocoel colonization remains explored insufficiently. Here, we report an indispensability of Cre1 for <i>Beauveria</i><i>bassiana</i>’s utilization of nutrients in insect integument and hemocoel. Deletion of <i>cre1</i> resulted in severe defects in radial growth on various media, hypersensitivity to oxidative stress, abolished pathogenicity via NCI or intrahemocoel injection (cuticle-bypassing infection) but no change in conidial hydrophobicity and adherence to insect cuticle. Markedly reduced biomass accumulation in the Δ<i>cre1</i> cultures was directly causative of severe defect in aerial conidiation and reduced secretion of various cuticle-degrading enzymes. The majority (1117) of 1881 dysregulated genes identified from the Δ<i>cre1</i> versus wild-type cultures were significantly downregulated, leading to substantial repression of many enriched function terms and pathways, particularly those involved in carbon and nitrogen metabolisms, cuticle degradation, antioxidant response, cellular transport and homeostasis, and direct/indirect gene mediation. These findings offer a novel insight into profound effect of Cre1 on the insect-pathogenic lifestyle of <i>B. bassiana</i>.
format	article
author	Rehab Abdelmonem Mohamed Kang Ren Ya-Ni Mou Sheng-Hua Ying Ming-Guang Feng
author_facet	Rehab Abdelmonem Mohamed Kang Ren Ya-Ni Mou Sheng-Hua Ying Ming-Guang Feng
author_sort	Rehab Abdelmonem Mohamed
title	Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>
title_short	Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>
title_full	Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>
title_fullStr	Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>
title_full_unstemmed	Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>
title_sort	genome-wide insight into profound effect of carbon catabolite repressor (cre1) on the insect-pathogenic lifecycle of <i>beauveria</i><i>bassiana</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3821e37ea4be4f0a9a49b895fc6a91a4
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Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of <i>Beauveria</i><i>bassiana</i>

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