Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution

Candida antarctica lipase B (CALB) is a serine lipase. Here, the authors use directed evolution to exchange serine with cysteine in the catalytic triad of the enzyme, thereby obtaining a highly active CALB variant that — unlike the wild type — accommodates bulky substrates.

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Autores principales:	Yixin Cen, Warispreet Singh, Mamatjan Arkin, Thomas S. Moody, Meilan Huang, Jiahai Zhou, Qi Wu, Manfred T. Reetz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/382d844422024dc1aad5ab0dfe4e992c
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spelling	oai:doaj.org-article:382d844422024dc1aad5ab0dfe4e992c2021-12-02T15:35:14ZArtificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution10.1038/s41467-019-11155-32041-1723https://doaj.org/article/382d844422024dc1aad5ab0dfe4e992c2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11155-3https://doaj.org/toc/2041-1723Candida antarctica lipase B (CALB) is a serine lipase. Here, the authors use directed evolution to exchange serine with cysteine in the catalytic triad of the enzyme, thereby obtaining a highly active CALB variant that — unlike the wild type — accommodates bulky substrates.Yixin CenWarispreet SinghMamatjan ArkinThomas S. MoodyMeilan HuangJiahai ZhouQi WuManfred T. ReetzNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yixin Cen Warispreet Singh Mamatjan Arkin Thomas S. Moody Meilan Huang Jiahai Zhou Qi Wu Manfred T. Reetz Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
description	Candida antarctica lipase B (CALB) is a serine lipase. Here, the authors use directed evolution to exchange serine with cysteine in the catalytic triad of the enzyme, thereby obtaining a highly active CALB variant that — unlike the wild type — accommodates bulky substrates.
format	article
author	Yixin Cen Warispreet Singh Mamatjan Arkin Thomas S. Moody Meilan Huang Jiahai Zhou Qi Wu Manfred T. Reetz
author_facet	Yixin Cen Warispreet Singh Mamatjan Arkin Thomas S. Moody Meilan Huang Jiahai Zhou Qi Wu Manfred T. Reetz
author_sort	Yixin Cen
title	Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
title_short	Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
title_full	Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
title_fullStr	Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
title_full_unstemmed	Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
title_sort	artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/382d844422024dc1aad5ab0dfe4e992c
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Artificial cysteine-lipases with high activity and altered catalytic mechanism created by laboratory evolution

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