Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.

An important layer of plant innate immunity to host-adapted pathogens is conferred by intracellular nucleotide-binding/oligomerization domain-leucine rich repeat (NB-LRR) receptors recognizing specific microbial effectors. Signaling from activated receptors of the TIR (Toll/Interleukin-1 Receptor)-N...

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Autores principales:	Ana V García, Servane Blanvillain-Baufumé, Robin P Huibers, Marcel Wiermer, Guangyong Li, Enrico Gobbato, Steffen Rietz, Jane E Parker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/382e689128f1413281a9a53120f682d1
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spelling	oai:doaj.org-article:382e689128f1413281a9a53120f682d12021-12-02T20:00:32ZBalanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.1553-73661553-737410.1371/journal.ppat.1000970https://doaj.org/article/382e689128f1413281a9a53120f682d12010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20617163/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374An important layer of plant innate immunity to host-adapted pathogens is conferred by intracellular nucleotide-binding/oligomerization domain-leucine rich repeat (NB-LRR) receptors recognizing specific microbial effectors. Signaling from activated receptors of the TIR (Toll/Interleukin-1 Receptor)-NB-LRR class converges on the nucleo-cytoplasmic immune regulator EDS1 (Enhanced Disease Susceptibility1). In this report we show that a receptor-stimulated increase in accumulation of nuclear EDS1 precedes or coincides with the EDS1-dependent induction and repression of defense-related genes. EDS1 is capable of nuclear transport receptor-mediated shuttling between the cytoplasm and nucleus. By enhancing EDS1 export from inside nuclei (through attachment of an additional nuclear export sequence (NES)) or conditionally releasing EDS1 to the nucleus (by fusion to a glucocorticoid receptor (GR)) in transgenic Arabidopsis we establish that the EDS1 nuclear pool is essential for resistance to biotrophic and hemi-biotrophic pathogens and for transcriptional reprogramming. Evidence points to post-transcriptional processes regulating receptor-triggered accumulation of EDS1 in nuclei. Changes in nuclear EDS1 levels become equilibrated with the cytoplasmic EDS1 pool and cytoplasmic EDS1 is needed for complete resistance and restriction of host cell death at infection sites. We propose that coordinated nuclear and cytoplasmic activities of EDS1 enable the plant to mount an appropriately balanced immune response to pathogen attack.Ana V GarcíaServane Blanvillain-BaufuméRobin P HuibersMarcel WiermerGuangyong LiEnrico GobbatoSteffen RietzJane E ParkerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, p e1000970 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Ana V García Servane Blanvillain-Baufumé Robin P Huibers Marcel Wiermer Guangyong Li Enrico Gobbato Steffen Rietz Jane E Parker Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.
description	An important layer of plant innate immunity to host-adapted pathogens is conferred by intracellular nucleotide-binding/oligomerization domain-leucine rich repeat (NB-LRR) receptors recognizing specific microbial effectors. Signaling from activated receptors of the TIR (Toll/Interleukin-1 Receptor)-NB-LRR class converges on the nucleo-cytoplasmic immune regulator EDS1 (Enhanced Disease Susceptibility1). In this report we show that a receptor-stimulated increase in accumulation of nuclear EDS1 precedes or coincides with the EDS1-dependent induction and repression of defense-related genes. EDS1 is capable of nuclear transport receptor-mediated shuttling between the cytoplasm and nucleus. By enhancing EDS1 export from inside nuclei (through attachment of an additional nuclear export sequence (NES)) or conditionally releasing EDS1 to the nucleus (by fusion to a glucocorticoid receptor (GR)) in transgenic Arabidopsis we establish that the EDS1 nuclear pool is essential for resistance to biotrophic and hemi-biotrophic pathogens and for transcriptional reprogramming. Evidence points to post-transcriptional processes regulating receptor-triggered accumulation of EDS1 in nuclei. Changes in nuclear EDS1 levels become equilibrated with the cytoplasmic EDS1 pool and cytoplasmic EDS1 is needed for complete resistance and restriction of host cell death at infection sites. We propose that coordinated nuclear and cytoplasmic activities of EDS1 enable the plant to mount an appropriately balanced immune response to pathogen attack.
format	article
author	Ana V García Servane Blanvillain-Baufumé Robin P Huibers Marcel Wiermer Guangyong Li Enrico Gobbato Steffen Rietz Jane E Parker
author_facet	Ana V García Servane Blanvillain-Baufumé Robin P Huibers Marcel Wiermer Guangyong Li Enrico Gobbato Steffen Rietz Jane E Parker
author_sort	Ana V García
title	Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.
title_short	Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.
title_full	Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.
title_fullStr	Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.
title_full_unstemmed	Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.
title_sort	balanced nuclear and cytoplasmic activities of eds1 are required for a complete plant innate immune response.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/382e689128f1413281a9a53120f682d1
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Balanced nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response.

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