The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.

Hosts have developed diverse mechanisms to counter the pathogens they face in their natural environment. Throughout the plant and animal kingdoms, the up-regulation of antimicrobial peptides is a common response to infection. In C. elegans, infection with the natural pathogen Drechmeria coniospora l...

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Autores principales:	Sid Ahmed Labed, Shizue Omi, Martha Gut, Jonathan J Ewbank, Nathalie Pujol
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38326f34fcbe42b9860ff4afbfe2103e
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spelling	oai:doaj.org-article:38326f34fcbe42b9860ff4afbfe2103e2021-11-18T07:24:32ZThe pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.1932-620310.1371/journal.pone.0033887https://doaj.org/article/38326f34fcbe42b9860ff4afbfe2103e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22470487/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hosts have developed diverse mechanisms to counter the pathogens they face in their natural environment. Throughout the plant and animal kingdoms, the up-regulation of antimicrobial peptides is a common response to infection. In C. elegans, infection with the natural pathogen Drechmeria coniospora leads to rapid induction of antimicrobial peptide gene expression in the epidermis. Through a large genetic screen we have isolated many new mutants that are incapable of upregulating the antimicrobial peptide nlp-29 in response to infection (i.e. with a Nipi or 'no induction of peptide after infection' phenotype). More than half of the newly isolated Nipi mutants do not correspond to genes previously associated with the regulation of antimicrobial peptides. One of these, nipi-4, encodes a member of a nematode-specific kinase family. NIPI-4 is predicted to be catalytically inactive, thus to be a pseudokinase. It acts in the epidermis downstream of the PKC∂ TPA-1, as a positive regulator of nlp antimicrobial peptide gene expression after infection. It also controls the constitutive expression of antimicrobial peptide genes of the cnc family that are targets of TGFß regulation. Our results open the way for a more detailed understanding of how host defense pathways can be molded by environmental pathogens.Sid Ahmed LabedShizue OmiMartha GutJonathan J EwbankNathalie PujolPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33887 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sid Ahmed Labed Shizue Omi Martha Gut Jonathan J Ewbank Nathalie Pujol The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.
description	Hosts have developed diverse mechanisms to counter the pathogens they face in their natural environment. Throughout the plant and animal kingdoms, the up-regulation of antimicrobial peptides is a common response to infection. In C. elegans, infection with the natural pathogen Drechmeria coniospora leads to rapid induction of antimicrobial peptide gene expression in the epidermis. Through a large genetic screen we have isolated many new mutants that are incapable of upregulating the antimicrobial peptide nlp-29 in response to infection (i.e. with a Nipi or 'no induction of peptide after infection' phenotype). More than half of the newly isolated Nipi mutants do not correspond to genes previously associated with the regulation of antimicrobial peptides. One of these, nipi-4, encodes a member of a nematode-specific kinase family. NIPI-4 is predicted to be catalytically inactive, thus to be a pseudokinase. It acts in the epidermis downstream of the PKC∂ TPA-1, as a positive regulator of nlp antimicrobial peptide gene expression after infection. It also controls the constitutive expression of antimicrobial peptide genes of the cnc family that are targets of TGFß regulation. Our results open the way for a more detailed understanding of how host defense pathways can be molded by environmental pathogens.
format	article
author	Sid Ahmed Labed Shizue Omi Martha Gut Jonathan J Ewbank Nathalie Pujol
author_facet	Sid Ahmed Labed Shizue Omi Martha Gut Jonathan J Ewbank Nathalie Pujol
author_sort	Sid Ahmed Labed
title	The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.
title_short	The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.
title_full	The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.
title_fullStr	The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.
title_full_unstemmed	The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.
title_sort	pseudokinase nipi-4 is a novel regulator of antimicrobial peptide gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/38326f34fcbe42b9860ff4afbfe2103e
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The pseudokinase NIPI-4 is a novel regulator of antimicrobial peptide gene expression.

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