Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.

Horizontal gene transfer greatly facilitates rapid genetic adaptation of bacteria to shifts in environmental conditions and colonization of new niches by allowing one-step acquisition of novel functions. Conjugation is a major mechanism of horizontal gene transfer mediated by conjugative plasmids an...

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Autores principales:	Mariana Gabriela Ghinet, Eric Bordeleau, Julie Beaudin, Ryszard Brzezinski, Sébastien Roy, Vincent Burrus
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/383f342dd220447bb2fef22f0024d00a
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spelling	oai:doaj.org-article:383f342dd220447bb2fef22f0024d00a2021-11-18T07:34:03ZUncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.1932-620310.1371/journal.pone.0027846https://doaj.org/article/383f342dd220447bb2fef22f0024d00a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22114709/?tool=EBIhttps://doaj.org/toc/1932-6203Horizontal gene transfer greatly facilitates rapid genetic adaptation of bacteria to shifts in environmental conditions and colonization of new niches by allowing one-step acquisition of novel functions. Conjugation is a major mechanism of horizontal gene transfer mediated by conjugative plasmids and integrating conjugative elements (ICEs). While in most bacterial conjugative systems DNA translocation requires the assembly of a complex type IV secretion system (T4SS), in Actinobacteria a single DNA FtsK/SpoIIIE-like translocation protein is required. To date, the role and diversity of ICEs in Actinobacteria have received little attention. Putative ICEs were searched for in 275 genomes of Actinobacteria using HMM-profiles of proteins involved in ICE maintenance and transfer. These exhaustive analyses revealed 144 putative FtsK/SpoIIIE-type ICEs and 17 putative T4SS-type ICEs. Grouping of the ICEs based on the phylogenetic analyses of maintenance and transfer proteins revealed extensive exchanges between different sub-families of ICEs. 17 ICEs were found in Actinobacteria from the genus Frankia, globally important nitrogen-fixing microorganisms that establish root nodule symbioses with actinorhizal plants. Structural analysis of ICEs from Frankia revealed their unexpected diversity and a vast array of predicted adaptive functions. Frankia ICEs were found to excise by site-specific recombination from their host's chromosome in vitro and in planta suggesting that they are functional mobile elements whether Frankiae live as soil saprophytes or plant endosymbionts. Phylogenetic analyses of proteins involved in ICEs maintenance and transfer suggests that active exchange between ICEs cargo-borne and chromosomal genes took place within the Actinomycetales order. Functionality of Frankia ICEs in vitro as well as in planta lets us anticipate that conjugation and ICEs could allow the development of genetic manipulation tools for this challenging microorganism and for many other Actinobacteria.Mariana Gabriela GhinetEric BordeleauJulie BeaudinRyszard BrzezinskiSébastien RoyVincent BurrusPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27846 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariana Gabriela Ghinet Eric Bordeleau Julie Beaudin Ryszard Brzezinski Sébastien Roy Vincent Burrus Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
description	Horizontal gene transfer greatly facilitates rapid genetic adaptation of bacteria to shifts in environmental conditions and colonization of new niches by allowing one-step acquisition of novel functions. Conjugation is a major mechanism of horizontal gene transfer mediated by conjugative plasmids and integrating conjugative elements (ICEs). While in most bacterial conjugative systems DNA translocation requires the assembly of a complex type IV secretion system (T4SS), in Actinobacteria a single DNA FtsK/SpoIIIE-like translocation protein is required. To date, the role and diversity of ICEs in Actinobacteria have received little attention. Putative ICEs were searched for in 275 genomes of Actinobacteria using HMM-profiles of proteins involved in ICE maintenance and transfer. These exhaustive analyses revealed 144 putative FtsK/SpoIIIE-type ICEs and 17 putative T4SS-type ICEs. Grouping of the ICEs based on the phylogenetic analyses of maintenance and transfer proteins revealed extensive exchanges between different sub-families of ICEs. 17 ICEs were found in Actinobacteria from the genus Frankia, globally important nitrogen-fixing microorganisms that establish root nodule symbioses with actinorhizal plants. Structural analysis of ICEs from Frankia revealed their unexpected diversity and a vast array of predicted adaptive functions. Frankia ICEs were found to excise by site-specific recombination from their host's chromosome in vitro and in planta suggesting that they are functional mobile elements whether Frankiae live as soil saprophytes or plant endosymbionts. Phylogenetic analyses of proteins involved in ICEs maintenance and transfer suggests that active exchange between ICEs cargo-borne and chromosomal genes took place within the Actinomycetales order. Functionality of Frankia ICEs in vitro as well as in planta lets us anticipate that conjugation and ICEs could allow the development of genetic manipulation tools for this challenging microorganism and for many other Actinobacteria.
format	article
author	Mariana Gabriela Ghinet Eric Bordeleau Julie Beaudin Ryszard Brzezinski Sébastien Roy Vincent Burrus
author_facet	Mariana Gabriela Ghinet Eric Bordeleau Julie Beaudin Ryszard Brzezinski Sébastien Roy Vincent Burrus
author_sort	Mariana Gabriela Ghinet
title	Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
title_short	Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
title_full	Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
title_fullStr	Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
title_full_unstemmed	Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
title_sort	uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/383f342dd220447bb2fef22f0024d00a
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Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.

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