Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids

Abstract This work focuses on modelling design and operation of “microfluidic sample traps” (MSTs). MSTs regroup a widely used class of microdevices that incorporate wells, recesses or chambers adjacent to a channel to individually trap, culture and/or release submicroliter 3D tissue samples ranging...

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Autores principales:	Nassim Rousset, Frédéric Monet, Thomas Gervais
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/384b97b1e00f49d9aac560c7aaee25d8
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spelling	oai:doaj.org-article:384b97b1e00f49d9aac560c7aaee25d82021-12-02T16:06:33ZSimulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids10.1038/s41598-017-00229-12045-2322https://doaj.org/article/384b97b1e00f49d9aac560c7aaee25d82017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00229-1https://doaj.org/toc/2045-2322Abstract This work focuses on modelling design and operation of “microfluidic sample traps” (MSTs). MSTs regroup a widely used class of microdevices that incorporate wells, recesses or chambers adjacent to a channel to individually trap, culture and/or release submicroliter 3D tissue samples ranging from simple cell aggregates and spheroids, to ex vivo tissue samples and other submillimetre-scale tissue models. Numerous MST designs employing various trapping mechanisms have been proposed in the literature, spurring the development of 3D tissue models for drug discovery and personalized medicine. Yet, there lacks a general framework to optimize trapping stability, trapping time, shear stress, and sample metabolism. Herein, the effects of hydrodynamics and diffusion-reaction on tissue viability and device operation are investigated using analytical and finite element methods with systematic parametric sweeps over independent design variables chosen to correspond to the four design degrees of freedom. Combining different results, we show that, for a spherical tissue of diameter d < 500 μm, the simplest, closest to optimal trap shape is a cube of dimensions w equal to twice the tissue diameter: w = 2d. Furthermore, to sustain tissues without perfusion, available medium volume per trap needs to be 100× the tissue volume to ensure optimal metabolism for at least 24 hours.Nassim RoussetFrédéric MonetThomas GervaisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nassim Rousset Frédéric Monet Thomas Gervais Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
description	Abstract This work focuses on modelling design and operation of “microfluidic sample traps” (MSTs). MSTs regroup a widely used class of microdevices that incorporate wells, recesses or chambers adjacent to a channel to individually trap, culture and/or release submicroliter 3D tissue samples ranging from simple cell aggregates and spheroids, to ex vivo tissue samples and other submillimetre-scale tissue models. Numerous MST designs employing various trapping mechanisms have been proposed in the literature, spurring the development of 3D tissue models for drug discovery and personalized medicine. Yet, there lacks a general framework to optimize trapping stability, trapping time, shear stress, and sample metabolism. Herein, the effects of hydrodynamics and diffusion-reaction on tissue viability and device operation are investigated using analytical and finite element methods with systematic parametric sweeps over independent design variables chosen to correspond to the four design degrees of freedom. Combining different results, we show that, for a spherical tissue of diameter d < 500 μm, the simplest, closest to optimal trap shape is a cube of dimensions w equal to twice the tissue diameter: w = 2d. Furthermore, to sustain tissues without perfusion, available medium volume per trap needs to be 100× the tissue volume to ensure optimal metabolism for at least 24 hours.
format	article
author	Nassim Rousset Frédéric Monet Thomas Gervais
author_facet	Nassim Rousset Frédéric Monet Thomas Gervais
author_sort	Nassim Rousset
title	Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
title_short	Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
title_full	Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
title_fullStr	Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
title_full_unstemmed	Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
title_sort	simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/384b97b1e00f49d9aac560c7aaee25d8
work_keys_str_mv	AT nassimrousset simulationassisteddesignofmicrofluidicsampletrapsforoptimaltrappingandcultureofnonadherentsinglecellstissuesandspheroids AT fredericmonet simulationassisteddesignofmicrofluidicsampletrapsforoptimaltrappingandcultureofnonadherentsinglecellstissuesandspheroids AT thomasgervais simulationassisteddesignofmicrofluidicsampletrapsforoptimaltrappingandcultureofnonadherentsinglecellstissuesandspheroids
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Simulation-assisted design of microfluidic sample traps for optimal trapping and culture of non-adherent single cells, tissues, and spheroids

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