Polymerase-endonuclease amplification reaction (PEAR) for large-scale enzymatic production of antisense oligonucleotides.
Antisense oligonucleotides targeting microRNAs or their mRNA targets prove to be powerful tools for molecular biology research and may eventually emerge as new therapeutic agents. Synthetic oligonucleotides are often contaminated with highly homologous failure sequences. Synthesis of a certain oligo...
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Formato: | article |
Lenguaje: | EN |
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Public Library of Science (PLoS)
2010
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Acceso en línea: | https://doaj.org/article/384c7d9c97bf43b6bb50c1092c81b81b |
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