Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells
Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cell...
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Nature Portfolio
2021
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oai:doaj.org-article:384d53bd750d40939873a02a0ac551262021-12-02T18:15:34ZCell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells10.1038/s41598-021-87319-32045-2322https://doaj.org/article/384d53bd750d40939873a02a0ac551262021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87319-3https://doaj.org/toc/2045-2322Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage.Marcos PenedoTetsuya ShirokawaMohammad Shahidul AlamKeisuke MiyazawaTakehiko IchikawaNaoko OkanoHirotoshi FurushoChikashi NakamuraTakeshi FukumaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
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Medicine R Science Q Marcos Penedo Tetsuya Shirokawa Mohammad Shahidul Alam Keisuke Miyazawa Takehiko Ichikawa Naoko Okano Hirotoshi Furusho Chikashi Nakamura Takeshi Fukuma Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| description |
Abstract Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or they can be employed to select and sort in parallel a large number of living cells. When using these nanoprobes, the most important requirement is to minimize the cell damage, reducing the forces and indentation lengths needed to penetrate the cell membrane. This is normally achieved by reducing the diameter of the nanoneedles. However, several studies have shown that nanoneedles with a flat tip display lower penetration forces and indentation lengths. In this work, we have tested different nanoneedle shapes and diameters to reduce the force and the indentation length needed to penetrate the cell membrane, demonstrating that ultra-thin and sharp nanoprobes can further reduce them, consequently minimizing the cell damage. |
| format |
article |
| author |
Marcos Penedo Tetsuya Shirokawa Mohammad Shahidul Alam Keisuke Miyazawa Takehiko Ichikawa Naoko Okano Hirotoshi Furusho Chikashi Nakamura Takeshi Fukuma |
| author_facet |
Marcos Penedo Tetsuya Shirokawa Mohammad Shahidul Alam Keisuke Miyazawa Takehiko Ichikawa Naoko Okano Hirotoshi Furusho Chikashi Nakamura Takeshi Fukuma |
| author_sort |
Marcos Penedo |
| title |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| title_short |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| title_full |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| title_fullStr |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| title_full_unstemmed |
Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| title_sort |
cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/384d53bd750d40939873a02a0ac55126 |
| work_keys_str_mv |
AT marcospenedo cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT tetsuyashirokawa cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT mohammadshahidulalam cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT keisukemiyazawa cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT takehikoichikawa cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT naokookano cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT hirotoshifurusho cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT chikashinakamura cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells AT takeshifukuma cellpenetrationefficiencyanalysisofdifferentatomicforcemicroscopynanoneedlesintolivingcells |
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1718378327663706112 |