Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have develo...
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eLife Sciences Publications Ltd
2021
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oai:doaj.org-article:384de1ef6af044c38f1f457657983e062021-11-30T08:03:22ZMonitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle10.7554/eLife.731862050-084Xe73186https://doaj.org/article/384de1ef6af044c38f1f457657983e062021-11-01T00:00:00Zhttps://elifesciences.org/articles/73186https://doaj.org/toc/2050-084XThe life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have developed an integrated microfluidic device to address this question, enabling continuous single-cell tracking in a batch culture experiencing unperturbed nutrient exhaustion to unravel the coordination between metabolic and structural transitions within cells. Our technique reveals an abrupt fate divergence in the population, whereby a fraction of cells is unable to transition to respiratory metabolism and undergoes a reversible entry into a quiescence-like state leading to premature cell death. Further observations reveal that nonmonotonous internal pH fluctuations in respiration-competent cells orchestrate the successive waves of protein superassemblies formation that accompany the entry into a bona fide quiescent state. This ultimately leads to an abrupt cytosolic glass transition that occurs stochastically long after proliferation cessation. This new experimental framework provides a unique way to track single-cell fate dynamics over a long timescale in a population of cells that continuously modify their ecological niche.Basile JacquelThéo AspertDamien LaporteIsabelle SagotGilles CharvineLife Sciences Publications Ltdarticlequiescencemicrofluidicssingle-cell dynamicscytosolic pHMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021) |
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DOAJ |
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EN |
| topic |
quiescence microfluidics single-cell dynamics cytosolic pH Medicine R Science Q Biology (General) QH301-705.5 |
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quiescence microfluidics single-cell dynamics cytosolic pH Medicine R Science Q Biology (General) QH301-705.5 Basile Jacquel Théo Aspert Damien Laporte Isabelle Sagot Gilles Charvin Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| description |
The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have developed an integrated microfluidic device to address this question, enabling continuous single-cell tracking in a batch culture experiencing unperturbed nutrient exhaustion to unravel the coordination between metabolic and structural transitions within cells. Our technique reveals an abrupt fate divergence in the population, whereby a fraction of cells is unable to transition to respiratory metabolism and undergoes a reversible entry into a quiescence-like state leading to premature cell death. Further observations reveal that nonmonotonous internal pH fluctuations in respiration-competent cells orchestrate the successive waves of protein superassemblies formation that accompany the entry into a bona fide quiescent state. This ultimately leads to an abrupt cytosolic glass transition that occurs stochastically long after proliferation cessation. This new experimental framework provides a unique way to track single-cell fate dynamics over a long timescale in a population of cells that continuously modify their ecological niche. |
| format |
article |
| author |
Basile Jacquel Théo Aspert Damien Laporte Isabelle Sagot Gilles Charvin |
| author_facet |
Basile Jacquel Théo Aspert Damien Laporte Isabelle Sagot Gilles Charvin |
| author_sort |
Basile Jacquel |
| title |
Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| title_short |
Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| title_full |
Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| title_fullStr |
Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| title_full_unstemmed |
Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| title_sort |
monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle |
| publisher |
eLife Sciences Publications Ltd |
| publishDate |
2021 |
| url |
https://doaj.org/article/384de1ef6af044c38f1f457657983e06 |
| work_keys_str_mv |
AT basilejacquel monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle AT theoaspert monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle AT damienlaporte monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle AT isabellesagot monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle AT gillescharvin monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle |
| _version_ |
1718406737701109760 |