Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle

The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have develo...

Description complète

Enregistré dans:
Détails bibliographiques
Auteurs principaux: Basile Jacquel, Théo Aspert, Damien Laporte, Isabelle Sagot, Gilles Charvin
Format: article
Langue:EN
Publié: eLife Sciences Publications Ltd 2021
Sujets:
R
Q
Accès en ligne:https://doaj.org/article/384de1ef6af044c38f1f457657983e06
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
id oai:doaj.org-article:384de1ef6af044c38f1f457657983e06
record_format dspace
spelling oai:doaj.org-article:384de1ef6af044c38f1f457657983e062021-11-30T08:03:22ZMonitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle10.7554/eLife.731862050-084Xe73186https://doaj.org/article/384de1ef6af044c38f1f457657983e062021-11-01T00:00:00Zhttps://elifesciences.org/articles/73186https://doaj.org/toc/2050-084XThe life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have developed an integrated microfluidic device to address this question, enabling continuous single-cell tracking in a batch culture experiencing unperturbed nutrient exhaustion to unravel the coordination between metabolic and structural transitions within cells. Our technique reveals an abrupt fate divergence in the population, whereby a fraction of cells is unable to transition to respiratory metabolism and undergoes a reversible entry into a quiescence-like state leading to premature cell death. Further observations reveal that nonmonotonous internal pH fluctuations in respiration-competent cells orchestrate the successive waves of protein superassemblies formation that accompany the entry into a bona fide quiescent state. This ultimately leads to an abrupt cytosolic glass transition that occurs stochastically long after proliferation cessation. This new experimental framework provides a unique way to track single-cell fate dynamics over a long timescale in a population of cells that continuously modify their ecological niche.Basile JacquelThéo AspertDamien LaporteIsabelle SagotGilles CharvineLife Sciences Publications Ltdarticlequiescencemicrofluidicssingle-cell dynamicscytosolic pHMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution DOAJ
collection DOAJ
language EN
topic quiescence
microfluidics
single-cell dynamics
cytosolic pH
Medicine
R
Science
Q
Biology (General)
QH301-705.5
spellingShingle quiescence
microfluidics
single-cell dynamics
cytosolic pH
Medicine
R
Science
Q
Biology (General)
QH301-705.5
Basile Jacquel
Théo Aspert
Damien Laporte
Isabelle Sagot
Gilles Charvin
Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
description The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive choreography of structural reorganizations observed in quiescent cells during a natural life cycle remains unclear. We have developed an integrated microfluidic device to address this question, enabling continuous single-cell tracking in a batch culture experiencing unperturbed nutrient exhaustion to unravel the coordination between metabolic and structural transitions within cells. Our technique reveals an abrupt fate divergence in the population, whereby a fraction of cells is unable to transition to respiratory metabolism and undergoes a reversible entry into a quiescence-like state leading to premature cell death. Further observations reveal that nonmonotonous internal pH fluctuations in respiration-competent cells orchestrate the successive waves of protein superassemblies formation that accompany the entry into a bona fide quiescent state. This ultimately leads to an abrupt cytosolic glass transition that occurs stochastically long after proliferation cessation. This new experimental framework provides a unique way to track single-cell fate dynamics over a long timescale in a population of cells that continuously modify their ecological niche.
format article
author Basile Jacquel
Théo Aspert
Damien Laporte
Isabelle Sagot
Gilles Charvin
author_facet Basile Jacquel
Théo Aspert
Damien Laporte
Isabelle Sagot
Gilles Charvin
author_sort Basile Jacquel
title Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
title_short Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
title_full Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
title_fullStr Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
title_full_unstemmed Monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
title_sort monitoring single-cell dynamics of entry into quiescence during an unperturbed life cycle
publisher eLife Sciences Publications Ltd
publishDate 2021
url https://doaj.org/article/384de1ef6af044c38f1f457657983e06
work_keys_str_mv AT basilejacquel monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle
AT theoaspert monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle
AT damienlaporte monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle
AT isabellesagot monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle
AT gillescharvin monitoringsinglecelldynamicsofentryintoquiescenceduringanunperturbedlifecycle
_version_ 1718406737701109760