Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields

Abstract Among different therapeutic applications of Ultrasound (US), transient membrane sonoporation (SP) - a temporary, non-lethal porosity, mechanically induced in cell membranes through US exposure - represents a compelling opportunity towards an efficient and safe drug delivery. Nevertheless, p...

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Autores principales:	F. Domenici, F. Brasili, S. Giantulli, B. Cerroni, A. Bedini, C. Giliberti, R. Palomba, I. Silvestri, S. Morrone, G. Paradossi, M. Mattei, F. Bordi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38537f7ccd0a46c2b8eae0eb0008ae3e
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spelling	oai:doaj.org-article:38537f7ccd0a46c2b8eae0eb0008ae3e2021-12-02T15:05:40ZDifferential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields10.1038/s41598-017-16708-42045-2322https://doaj.org/article/38537f7ccd0a46c2b8eae0eb0008ae3e2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-16708-4https://doaj.org/toc/2045-2322Abstract Among different therapeutic applications of Ultrasound (US), transient membrane sonoporation (SP) - a temporary, non-lethal porosity, mechanically induced in cell membranes through US exposure - represents a compelling opportunity towards an efficient and safe drug delivery. Nevertheless, progresses in this field have been limited by an insufficient understanding of the potential cytotoxic effects of US related to the failure of the cellular repair and to the possible activation of inflammatory pathway. In this framework we studied the in vitro effects of very low-intensity US on a human keratinocyte cell line, which represents an ideal model system of skin protective barrier cells which are the first to be involved during medical US treatments. Bioeffects linked to US application at 1 MHz varying the exposure parameters were investigated by fluorescence microscopy and fluorescence activated cell sorting. Our results indicate that keratinocytes undergoing low US doses can uptake drug model molecules with size and efficiency which depend on exposure parameters. According to sub-cavitation SP models, we have identified the range of doses triggering transient membrane SP, actually with negligible biological damage. By increasing US doses we observed a reduced cells viability and an inflammatory gene overexpression enlightening novel healthy relevant strategies.F. DomeniciF. BrasiliS. GiantulliB. CerroniA. BediniC. GilibertiR. PalombaI. SilvestriS. MorroneG. ParadossiM. MatteiF. BordiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q F. Domenici F. Brasili S. Giantulli B. Cerroni A. Bedini C. Giliberti R. Palomba I. Silvestri S. Morrone G. Paradossi M. Mattei F. Bordi Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
description	Abstract Among different therapeutic applications of Ultrasound (US), transient membrane sonoporation (SP) - a temporary, non-lethal porosity, mechanically induced in cell membranes through US exposure - represents a compelling opportunity towards an efficient and safe drug delivery. Nevertheless, progresses in this field have been limited by an insufficient understanding of the potential cytotoxic effects of US related to the failure of the cellular repair and to the possible activation of inflammatory pathway. In this framework we studied the in vitro effects of very low-intensity US on a human keratinocyte cell line, which represents an ideal model system of skin protective barrier cells which are the first to be involved during medical US treatments. Bioeffects linked to US application at 1 MHz varying the exposure parameters were investigated by fluorescence microscopy and fluorescence activated cell sorting. Our results indicate that keratinocytes undergoing low US doses can uptake drug model molecules with size and efficiency which depend on exposure parameters. According to sub-cavitation SP models, we have identified the range of doses triggering transient membrane SP, actually with negligible biological damage. By increasing US doses we observed a reduced cells viability and an inflammatory gene overexpression enlightening novel healthy relevant strategies.
format	article
author	F. Domenici F. Brasili S. Giantulli B. Cerroni A. Bedini C. Giliberti R. Palomba I. Silvestri S. Morrone G. Paradossi M. Mattei F. Bordi
author_facet	F. Domenici F. Brasili S. Giantulli B. Cerroni A. Bedini C. Giliberti R. Palomba I. Silvestri S. Morrone G. Paradossi M. Mattei F. Bordi
author_sort	F. Domenici
title	Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
title_short	Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
title_full	Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
title_fullStr	Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
title_full_unstemmed	Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
title_sort	differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/38537f7ccd0a46c2b8eae0eb0008ae3e
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Differential effects on membrane permeability and viability of human keratinocyte cells undergoing very low intensity megasonic fields

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