Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae

Abstract Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy. All these techniques suffer from anisotropic resolution and limited penetration depth. In...

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Autores principales:	Dimitrios Kapsokalyvas, Rodrigo Rosas, Rob W. A. Janssen, Jo M. Vanoevelen, Miranda Nabben, Martin Strauch, Dorit Merhof, Marc A. M. J. van Zandvoort
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38577ad5a5eb4a9e84cbcd84b7270387
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spelling	oai:doaj.org-article:38577ad5a5eb4a9e84cbcd84b72703872021-12-02T17:15:36ZMultiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae10.1038/s41598-021-89566-w2045-2322https://doaj.org/article/38577ad5a5eb4a9e84cbcd84b72703872021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89566-whttps://doaj.org/toc/2045-2322Abstract Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy. All these techniques suffer from anisotropic resolution and limited penetration depth. In the past, Multiview microscopy—imaging the sample from different angles followed by 3D image reconstruction—was developed to address this issue for light sheet microscopy based on fluorescence signal. In this study we applied this methodology to accomplish Multiview imaging with multiphoton microscopy based on fluorescence and additionally second harmonic signal from myosin and collagen. It was shown that isotropic resolution was achieved, the entirety of the sample was visualized, and interference artifacts were suppressed allowing clear visualization of collagen fibrils and myofibrils. This method can be applied to any scanning microscopy technique without microscope modifications. It can be used for imaging tissue and whole mount small organisms such as heart tissue, and zebrafish larva in 3D, label-free or stained, with at least threefold axial resolution improvement which can be significant for the accurate quantification of small 3D structures.Dimitrios KapsokalyvasRodrigo RosasRob W. A. JanssenJo M. VanoevelenMiranda NabbenMartin StrauchDorit MerhofMarc A. M. J. van ZandvoortNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dimitrios Kapsokalyvas Rodrigo Rosas Rob W. A. Janssen Jo M. Vanoevelen Miranda Nabben Martin Strauch Dorit Merhof Marc A. M. J. van Zandvoort Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
description	Abstract Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy. All these techniques suffer from anisotropic resolution and limited penetration depth. In the past, Multiview microscopy—imaging the sample from different angles followed by 3D image reconstruction—was developed to address this issue for light sheet microscopy based on fluorescence signal. In this study we applied this methodology to accomplish Multiview imaging with multiphoton microscopy based on fluorescence and additionally second harmonic signal from myosin and collagen. It was shown that isotropic resolution was achieved, the entirety of the sample was visualized, and interference artifacts were suppressed allowing clear visualization of collagen fibrils and myofibrils. This method can be applied to any scanning microscopy technique without microscope modifications. It can be used for imaging tissue and whole mount small organisms such as heart tissue, and zebrafish larva in 3D, label-free or stained, with at least threefold axial resolution improvement which can be significant for the accurate quantification of small 3D structures.
format	article
author	Dimitrios Kapsokalyvas Rodrigo Rosas Rob W. A. Janssen Jo M. Vanoevelen Miranda Nabben Martin Strauch Dorit Merhof Marc A. M. J. van Zandvoort
author_facet	Dimitrios Kapsokalyvas Rodrigo Rosas Rob W. A. Janssen Jo M. Vanoevelen Miranda Nabben Martin Strauch Dorit Merhof Marc A. M. J. van Zandvoort
author_sort	Dimitrios Kapsokalyvas
title	Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
title_short	Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
title_full	Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
title_fullStr	Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
title_full_unstemmed	Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
title_sort	multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/38577ad5a5eb4a9e84cbcd84b7270387
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Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae

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