Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium

TREK-2-like channels in the pyramidal neurons of rat prefrontal cortex are characterized by a wide range of spontaneous activity—from very low to very high—independent of the membrane potential and the stimuli that are known to activate TREK-2 channels, such as temperature or membrane stretching. Th...

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Autores principales:	Beata Dworakowska, Maciej Gawlak, Ewa Nurowska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	TREK-2 channel calcium signaling PI(4,5)P2 signaling Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3858727bde9543e59d8e60c6187cc484
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id	oai:doaj.org-article:3858727bde9543e59d8e60c6187cc484
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spelling	oai:doaj.org-article:3858727bde9543e59d8e60c6187cc4842021-11-25T16:47:11ZActivity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium10.3390/biology101111192079-7737https://doaj.org/article/3858727bde9543e59d8e60c6187cc4842021-10-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1119https://doaj.org/toc/2079-7737TREK-2-like channels in the pyramidal neurons of rat prefrontal cortex are characterized by a wide range of spontaneous activity—from very low to very high—independent of the membrane potential and the stimuli that are known to activate TREK-2 channels, such as temperature or membrane stretching. The aim of this study was to discover what factors are involved in high levels of TREK-2-like channel activity in these cells. Our research focused on the PI(4,5)P2-dependent mechanism of channel activity. Single-channel patch clamp recordings were performed on freshly dissociated pyramidal neurons of rat prefrontal cortexes in both the cell-attached and inside-out configurations. To evaluate the role of endogenous stimulants, the activity of the channels was recorded in the presence of a PI(4,5)P2 analogue (PI(4,5)P2DiC8) and Ca<sup>2+</sup>. Our research revealed that calcium ions are an important factor affecting TREK-2-like channel activity and kinetics. The observation that calcium participates in the activation of TREK-2-like channels is a new finding. We showed that PI(4,5)P2-dependent TREK-2 activity occurs when the conditions for PI(4,5)P2/Ca<sup>2+</sup> nanocluster formation are met. We present a possible model explaining the mechanism of calcium action.Beata DworakowskaMaciej GawlakEwa NurowskaMDPI AGarticleTREK-2 channelcalcium signalingPI(4,5)P2 signalingBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1119, p 1119 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TREK-2 channel calcium signaling PI(4,5)P2 signaling Biology (General) QH301-705.5
spellingShingle	TREK-2 channel calcium signaling PI(4,5)P2 signaling Biology (General) QH301-705.5 Beata Dworakowska Maciej Gawlak Ewa Nurowska Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium
description	TREK-2-like channels in the pyramidal neurons of rat prefrontal cortex are characterized by a wide range of spontaneous activity—from very low to very high—independent of the membrane potential and the stimuli that are known to activate TREK-2 channels, such as temperature or membrane stretching. The aim of this study was to discover what factors are involved in high levels of TREK-2-like channel activity in these cells. Our research focused on the PI(4,5)P2-dependent mechanism of channel activity. Single-channel patch clamp recordings were performed on freshly dissociated pyramidal neurons of rat prefrontal cortexes in both the cell-attached and inside-out configurations. To evaluate the role of endogenous stimulants, the activity of the channels was recorded in the presence of a PI(4,5)P2 analogue (PI(4,5)P2DiC8) and Ca<sup>2+</sup>. Our research revealed that calcium ions are an important factor affecting TREK-2-like channel activity and kinetics. The observation that calcium participates in the activation of TREK-2-like channels is a new finding. We showed that PI(4,5)P2-dependent TREK-2 activity occurs when the conditions for PI(4,5)P2/Ca<sup>2+</sup> nanocluster formation are met. We present a possible model explaining the mechanism of calcium action.
format	article
author	Beata Dworakowska Maciej Gawlak Ewa Nurowska
author_facet	Beata Dworakowska Maciej Gawlak Ewa Nurowska
author_sort	Beata Dworakowska
title	Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium
title_short	Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium
title_full	Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium
title_fullStr	Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium
title_full_unstemmed	Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium
title_sort	activity of trek-2-like channels in the pyramidal neurons of rat medial prefrontal cortex depends on cytoplasmic calcium
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3858727bde9543e59d8e60c6187cc484
work_keys_str_mv	AT beatadworakowska activityoftrek2likechannelsinthepyramidalneuronsofratmedialprefrontalcortexdependsoncytoplasmiccalcium AT maciejgawlak activityoftrek2likechannelsinthepyramidalneuronsofratmedialprefrontalcortexdependsoncytoplasmiccalcium AT ewanurowska activityoftrek2likechannelsinthepyramidalneuronsofratmedialprefrontalcortexdependsoncytoplasmiccalcium
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Activity of TREK-2-like Channels in the Pyramidal Neurons of Rat Medial Prefrontal Cortex Depends on Cytoplasmic Calcium

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