A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling

Abstract Animal models of allergic airways inflammation are useful tools in studying the pathogenesis of asthma and potential therapeutic interventions. The different allergic airways inflammation models available to date employ varying doses, frequency, duration and types of allergen, which lead to...

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Autores principales:	L. N. Woo, W. Y. Guo, X. Wang, A. Young, S. Salehi, A. Hin, Y. Zhang, J. A. Scott, C. W. Chow
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38595dc599fb40ca9fefff319c69ccd6
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spelling	oai:doaj.org-article:38595dc599fb40ca9fefff319c69ccd62021-12-02T11:41:04ZA 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling10.1038/s41598-018-24574-x2045-2322https://doaj.org/article/38595dc599fb40ca9fefff319c69ccd62018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24574-xhttps://doaj.org/toc/2045-2322Abstract Animal models of allergic airways inflammation are useful tools in studying the pathogenesis of asthma and potential therapeutic interventions. The different allergic airways inflammation models available to date employ varying doses, frequency, duration and types of allergen, which lead to the development of different features of asthma; showing varying degrees of airways inflammation and hyper-responsiveness (AHR) and airways remodeling. Models that also exhibit airway remodeling, a key feature of asthma, in addition to AHR and airway inflammation typically require 5–12 weeks to develop. In this report, we describe a 4-week mouse model of house dust mite (HDM)-induced allergic airways inflammation, and compare the phenotypic features of two different doses of HDM exposures (10 µg and 25 µg) for 5 days/week with a well-characterized 8-week chronic HDM model. We found that 4 weeks of intranasal HDM (25 µg in 35 µl saline; 5 days/week) resulted in AHR, airway inflammation and airway remodeling that were comparable to the 8-week model. We conclude that this new 4-week HDM model is another useful tool in studies of human asthma that offers advantages of shorter duration for development and decreased costs when compared to other models that require longer durations of exposure (5–12 weeks) to develop.L. N. WooW. Y. GuoX. WangA. YoungS. SalehiA. HinY. ZhangJ. A. ScottC. W. ChowNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. N. Woo W. Y. Guo X. Wang A. Young S. Salehi A. Hin Y. Zhang J. A. Scott C. W. Chow A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling
description	Abstract Animal models of allergic airways inflammation are useful tools in studying the pathogenesis of asthma and potential therapeutic interventions. The different allergic airways inflammation models available to date employ varying doses, frequency, duration and types of allergen, which lead to the development of different features of asthma; showing varying degrees of airways inflammation and hyper-responsiveness (AHR) and airways remodeling. Models that also exhibit airway remodeling, a key feature of asthma, in addition to AHR and airway inflammation typically require 5–12 weeks to develop. In this report, we describe a 4-week mouse model of house dust mite (HDM)-induced allergic airways inflammation, and compare the phenotypic features of two different doses of HDM exposures (10 µg and 25 µg) for 5 days/week with a well-characterized 8-week chronic HDM model. We found that 4 weeks of intranasal HDM (25 µg in 35 µl saline; 5 days/week) resulted in AHR, airway inflammation and airway remodeling that were comparable to the 8-week model. We conclude that this new 4-week HDM model is another useful tool in studies of human asthma that offers advantages of shorter duration for development and decreased costs when compared to other models that require longer durations of exposure (5–12 weeks) to develop.
format	article
author	L. N. Woo W. Y. Guo X. Wang A. Young S. Salehi A. Hin Y. Zhang J. A. Scott C. W. Chow
author_facet	L. N. Woo W. Y. Guo X. Wang A. Young S. Salehi A. Hin Y. Zhang J. A. Scott C. W. Chow
author_sort	L. N. Woo
title	A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling
title_short	A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling
title_full	A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling
title_fullStr	A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling
title_full_unstemmed	A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling
title_sort	4-week model of house dust mite (hdm) induced allergic airways inflammation with airway remodeling
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/38595dc599fb40ca9fefff319c69ccd6
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A 4-Week Model of House Dust Mite (HDM) Induced Allergic Airways Inflammation with Airway Remodeling

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