Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells

Anderson Nogueira Mendes,1 Lívia Alves Filgueiras,1 Monica Regina Pimentel Siqueira,2,3 Gleyce Moreno Barbosa,2 Carla Holandino,2 Davyson de Lima Moreira,3 José Carlos Pinto,4 Marcio Nele5 1Department of Biophysics and Physiology, Federal University of Piauí, Teresi...

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Autores principales:	Mendes AN, Filgueiras LA, Siqueira MR, Barbosa GM, Holandino C, de Lima Moreira D, Pinto JC, Nele M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Piper Piperaceae nanoparticles miniemulsion PMMA K562 cells leukemia Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3859d040eeb34fb688c59bdb010c4923
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spelling	oai:doaj.org-article:3859d040eeb34fb688c59bdb010c49232021-12-02T07:43:10ZEncapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells1178-2013https://doaj.org/article/3859d040eeb34fb688c59bdb010c49232017-11-01T00:00:00Zhttps://www.dovepress.com/encapsulation-of-piper-cabralanum-piperaceae-nonpolar-extract-in-polym-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Anderson Nogueira Mendes,1 Lívia Alves Filgueiras,1 Monica Regina Pimentel Siqueira,2,3 Gleyce Moreno Barbosa,2 Carla Holandino,2 Davyson de Lima Moreira,3 José Carlos Pinto,4 Marcio Nele5 1Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Piauí, 2School of Pharmacy, Federal University of Rio Janeiro, 3Natural Products Department, Institute of Pharmaceutical Technology, Farmanguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, 4Chemical Engineering Program – COPPE, Federal University of Rio de Janeiro, Rio de Janeiro 5School of Chemistry, Federal University of Rio Janeiro, Rio de Janeiro, Brazil Abstract: This study aimed to synthesize and characterize nanoparticles (NPs) of poly(methyl methacrylate) (PMMA) and evaluate their ability to incorporate plant extracts with antitumor activity and low dissolution in aqueous media. The extract used was n-hexane partition of the methanol extract of Piper cabralanum (PCA-HEX). PMMA NPs were obtained using the miniemulsion method, which was able to encapsulate almost 100% of PCA-HEX. The synthesized polymeric particles presented with a size of 200 nm and a negative charge. Cytotoxicity tests by MTT and trypan blue assays showed that NPs without PCA-HEX did not kill leukemic cells (K562 cells). NPs containing PCA-HEX were able to enhance cell death when compared to pure extract. The results showed that PMMA NPs could be useful as a drug delivery system as they can enhance the antitumor activity of the PCA-HEX extract by more than 20-fold. PMMA NPs containing plant extracts with antitumor activities may be an alternative to control the evolution of diseases such as leukemia. Keywords: Piper, Piperaceae, nanoparticles, miniemulsion, PMMA, K562 cells, leukemiaMendes ANFilgueiras LASiqueira MRBarbosa GMHolandino Cde Lima Moreira DPinto JCNele MDove Medical PressarticlePiperPiperaceaenanoparticlesminiemulsionPMMAK562 cellsleukemiaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 8363-8373 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Piper Piperaceae nanoparticles miniemulsion PMMA K562 cells leukemia Medicine (General) R5-920
spellingShingle	Piper Piperaceae nanoparticles miniemulsion PMMA K562 cells leukemia Medicine (General) R5-920 Mendes AN Filgueiras LA Siqueira MR Barbosa GM Holandino C de Lima Moreira D Pinto JC Nele M Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
description	Anderson Nogueira Mendes,1 Lívia Alves Filgueiras,1 Monica Regina Pimentel Siqueira,2,3 Gleyce Moreno Barbosa,2 Carla Holandino,2 Davyson de Lima Moreira,3 José Carlos Pinto,4 Marcio Nele5 1Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Piauí, 2School of Pharmacy, Federal University of Rio Janeiro, 3Natural Products Department, Institute of Pharmaceutical Technology, Farmanguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, 4Chemical Engineering Program – COPPE, Federal University of Rio de Janeiro, Rio de Janeiro 5School of Chemistry, Federal University of Rio Janeiro, Rio de Janeiro, Brazil Abstract: This study aimed to synthesize and characterize nanoparticles (NPs) of poly(methyl methacrylate) (PMMA) and evaluate their ability to incorporate plant extracts with antitumor activity and low dissolution in aqueous media. The extract used was n-hexane partition of the methanol extract of Piper cabralanum (PCA-HEX). PMMA NPs were obtained using the miniemulsion method, which was able to encapsulate almost 100% of PCA-HEX. The synthesized polymeric particles presented with a size of 200 nm and a negative charge. Cytotoxicity tests by MTT and trypan blue assays showed that NPs without PCA-HEX did not kill leukemic cells (K562 cells). NPs containing PCA-HEX were able to enhance cell death when compared to pure extract. The results showed that PMMA NPs could be useful as a drug delivery system as they can enhance the antitumor activity of the PCA-HEX extract by more than 20-fold. PMMA NPs containing plant extracts with antitumor activities may be an alternative to control the evolution of diseases such as leukemia. Keywords: Piper, Piperaceae, nanoparticles, miniemulsion, PMMA, K562 cells, leukemia
format	article
author	Mendes AN Filgueiras LA Siqueira MR Barbosa GM Holandino C de Lima Moreira D Pinto JC Nele M
author_facet	Mendes AN Filgueiras LA Siqueira MR Barbosa GM Holandino C de Lima Moreira D Pinto JC Nele M
author_sort	Mendes AN
title	Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
title_short	Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
title_full	Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
title_fullStr	Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
title_full_unstemmed	Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells
title_sort	encapsulation of piper cabralanum (piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in k562 cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/3859d040eeb34fb688c59bdb010c4923
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Encapsulation of Piper cabralanum (Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells

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